Effect of high-fat buffalo dairy diets with different fatty acid composition on hepatic fatty degeneration in C57BL/6J mice

This work compared the effects of two diets high in milk fat of buffaloes with different fatty acid profiles on hepatic fatty degeneration in mice. Male C57BL/6J mice were fed for 154 days with balanced food (control) and two foods rich in milk fat (≈20%): one with a standard profile of fatty acids...

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Autores principales: Lertora, Walter Javier, Abson, G. S., Villordo, G. I., Sánchez Negrette, Marcial, Villarrubia, M. B.
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Nordeste 2023
Materias:
steatohepatitis
trans fats
ruminant
dairy fat
esteatohepatitis
grasas trans
rumiante
grasa láctea
Acceso en línea:https://revistas.unne.edu.ar/index.php/vet/article/view/7040
Aporte de:
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste
id I48-R154-article-7040
record_format ojs
spelling I48-R154-article-70402023-11-28T12:59:01Z Effect of high-fat buffalo dairy diets with different fatty acid composition on hepatic fatty degeneration in C57BL/6J mice Efecto de dietas altas en grasas lácteas bubalinas con diferente composición de ácidos grasos sobre la degeneración grasa hepática en ratones C57BL/6J Lertora, Walter Javier Abson, G. S. Villordo, G. I. Sánchez Negrette, Marcial Villarrubia, M. B. steatohepatitis trans fats ruminant dairy fat esteatohepatitis grasas trans rumiante grasa láctea This work compared the effects of two diets high in milk fat of buffaloes with different fatty acid profiles on hepatic fatty degeneration in mice. Male C57BL/6J mice were fed for 154 days with balanced food (control) and two foods rich in milk fat (≈20%): one with a standard profile of fatty acids obtained from buffaloes fed with natural grass (GLP) and another reduced in saturated fats (-31%), rich in rumenic acid (+212,5%) and trans fats (+434,2%, predominantly vaccenic acid) (GLE), obtained from buffaloes fed with natural grass + lipid supplementation. Nutritional parameters, weight gain, cholesterolemia, serum transaminases, liver fat content, and liver histopathology were evaluated. Compared with the control group, GLP and GLE mice were obese, had increased serum ALT, and developed severe liver fatty degeneration (>66% affected parenchyma) with lobular inflammation and hepatocellular ballooning. There weren`t differences between the GLP and GLE groups in food and calorie intake, weight gain, markers of liver damage, cholesterolemia, hepatic lipid content or in liver lesions. The level of fat in the diet and not the type of milk fat was responsible for the observed changes. In conclusion, both high-fat buffalo dairy foods had a similar effect and neither attenuated liver fat degeneration of C57BL/6J mice. Este trabajo comparó los efectos de dos dietas altas en grasas lácteas bubalinas con diferente perfil de ácidos grasos sobre la degeneración grasa hepática en ratones. Ratones C57BL/6J, machos, fueron alimentados durante 154 días con alimento balanceado (control) y con dos alimentos ricos en grasas lácteas (≈20%): uno con perfil estándar de ácidos grasos obtenido de búfalas alimentadas con pastura natural (GLP) y otro reducido en grasas saturadas (-31%), rico en ácido ruménico (+212,5%) y grasas trans (+434,2%, predominantemente ácido vaccénico), obtenido de búfalas alimentadas con pasto natural + suplementación lipídica (GLE). Se evaluaron parámetros nutricionales, ganancia de peso, colesterolemia, transaminasas séricas, contenido de grasa hepática e histopatología del hígado. Comparado con el grupo control, los ratones GLP y GLE fueron obesos, registraron incrementos séricos de ALT y desarrollaron una severa degeneración grasa hepática (>66% de parénquima afectado) con inflamación lobular y balonamiento hepatocelular. No hubo diferencias entre los grupos GLP y GLE en el consumo de alimento, las calorías ingeridas, la ganancia de peso, los marcadores de daño hepático, la colesterolemia, el contenido de lípidos hepático o en las lesiones hepáticas. El nivel de grasas en la dieta y no el tipo de grasa láctea fue responsable de los cambios observados. En conclusión, ambos alimentos altos en grasas lácteas bubalinas tuvieron similar efecto y ninguno atenuó la degeneración grasa hepática de ratones C57BL/6J. Universidad Nacional del Nordeste 2023-11-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/7040 10.30972/vet.3427040 Revista Veterinaria; Vol. 34 Núm. 2 (2023); 13-23 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/7040/6496 https://creativecommons.org/licenses/by/2.5/ar/
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-154
container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Español
format Artículo revista
topic steatohepatitis
trans fats
ruminant
dairy fat
esteatohepatitis
grasas trans
rumiante
grasa láctea
spellingShingle steatohepatitis
trans fats
ruminant
dairy fat
esteatohepatitis
grasas trans
rumiante
grasa láctea
Lertora, Walter Javier
Abson, G. S.
Villordo, G. I.
Sánchez Negrette, Marcial
Villarrubia, M. B.
Effect of high-fat buffalo dairy diets with different fatty acid composition on hepatic fatty degeneration in C57BL/6J mice
topic_facet steatohepatitis
trans fats
ruminant
dairy fat
esteatohepatitis
grasas trans
rumiante
grasa láctea
author Lertora, Walter Javier
Abson, G. S.
Villordo, G. I.
Sánchez Negrette, Marcial
Villarrubia, M. B.
author_facet Lertora, Walter Javier
Abson, G. S.
Villordo, G. I.
Sánchez Negrette, Marcial
Villarrubia, M. B.
author_sort Lertora, Walter Javier
title Effect of high-fat buffalo dairy diets with different fatty acid composition on hepatic fatty degeneration in C57BL/6J mice
title_short Effect of high-fat buffalo dairy diets with different fatty acid composition on hepatic fatty degeneration in C57BL/6J mice
title_full Effect of high-fat buffalo dairy diets with different fatty acid composition on hepatic fatty degeneration in C57BL/6J mice
title_fullStr Effect of high-fat buffalo dairy diets with different fatty acid composition on hepatic fatty degeneration in C57BL/6J mice
title_full_unstemmed Effect of high-fat buffalo dairy diets with different fatty acid composition on hepatic fatty degeneration in C57BL/6J mice
title_sort effect of high-fat buffalo dairy diets with different fatty acid composition on hepatic fatty degeneration in c57bl/6j mice
description This work compared the effects of two diets high in milk fat of buffaloes with different fatty acid profiles on hepatic fatty degeneration in mice. Male C57BL/6J mice were fed for 154 days with balanced food (control) and two foods rich in milk fat (≈20%): one with a standard profile of fatty acids obtained from buffaloes fed with natural grass (GLP) and another reduced in saturated fats (-31%), rich in rumenic acid (+212,5%) and trans fats (+434,2%, predominantly vaccenic acid) (GLE), obtained from buffaloes fed with natural grass + lipid supplementation. Nutritional parameters, weight gain, cholesterolemia, serum transaminases, liver fat content, and liver histopathology were evaluated. Compared with the control group, GLP and GLE mice were obese, had increased serum ALT, and developed severe liver fatty degeneration (>66% affected parenchyma) with lobular inflammation and hepatocellular ballooning. There weren`t differences between the GLP and GLE groups in food and calorie intake, weight gain, markers of liver damage, cholesterolemia, hepatic lipid content or in liver lesions. The level of fat in the diet and not the type of milk fat was responsible for the observed changes. In conclusion, both high-fat buffalo dairy foods had a similar effect and neither attenuated liver fat degeneration of C57BL/6J mice.
publisher Universidad Nacional del Nordeste
publishDate 2023
url https://revistas.unne.edu.ar/index.php/vet/article/view/7040
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first_indexed 2024-08-21T22:35:51Z
last_indexed 2024-08-21T22:35:51Z
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