In vitro culture of embryos isolated from seeds of immature fruits of irupé (Victoria cruziana, Nymphaeaceae)

The irupé or water corn (Victoria cruziana A. D. Orb.) belongs to the Nymphaeaceae family and arouses interest mainly due to the beauty of its leaves and flowers. Obtaining seeds of this aquatic species in its natural environment is challenging, and it is also considered a recalcitrant species for i...

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Autores principales: Terada, Graciela, Flachsland, Eduardo A., Medina, Ricardo D., Mignolli, Francesco, Vidoz, María L.
Formato: Artículo revista
Lenguaje:Español
Publicado: Instituto de Botánica del Nordeste 2023
Materias:
Aquatic plants
growth regulators
Nymphaeaceae
plant regeneration
recalcitrant
plantas acuáticas
recalcitrantes
regeneración de plantas
reguladores de crecimiento
Acceso en línea:https://revistas.unne.edu.ar/index.php/bon/article/view/6809
Aporte de:
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste
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spelling I48-R154-article-68092023-08-20T23:34:00Z In vitro culture of embryos isolated from seeds of immature fruits of irupé (Victoria cruziana, Nymphaeaceae) Cultivo in vitro de embriones aislados a partir de semillas de frutos inmaduros de irupé (Victoria cruziana, Nymphaeaceae) Terada, Graciela Flachsland, Eduardo A. Medina, Ricardo D. Mignolli, Francesco Vidoz, María L. Aquatic plants growth regulators Nymphaeaceae plant regeneration recalcitrant Nymphaeaceae plantas acuáticas recalcitrantes regeneración de plantas reguladores de crecimiento The irupé or water corn (Victoria cruziana A. D. Orb.) belongs to the Nymphaeaceae family and arouses interest mainly due to the beauty of its leaves and flowers. Obtaining seeds of this aquatic species in its natural environment is challenging, and it is also considered a recalcitrant species for in vitro culture. For this reason, the aim of this work was to establish a methodology for the conversion into plants of irupé embryos extracted from seeds of immature fruits and to obtain material to be used in micropropagation or in environmental restoration programs. Embryo culture of fruits in six growth stages was carried out up to a maximum of four weeks. Using Murashige & Skoog’s basal medium, twelve culture media with and without the addition of growth regulators (gibberellic acid, AG3 and thidiazuron, TDZ) were tested. Embryos converted into plants in all media diluted to 50% (½ MS) with 3% sucrose, while plants with developed roots were obtained in the medium with the addition of 10 mg/L of GA3 . Although the culture of embryos derived from seeds in early fruit stages did not allow plant regeneration, this was possible when explants derived from fruits in late developmental stages. El irupé o maíz de agua (Victoria cruziana A. D. Orb.) pertenece a la familia Nymphaeaceae y despierta interés, principalmente, por la belleza de sus hojas y flores. La obtención de semillas de esta especie acuática es dificultosa en su ambiente natural y además es considerada recalcitrante para el cultivo in vitro. Por este motivo, el objetivo de este trabajo fue el establecimiento de una metodología para la conversión a plantas de embriones de irupé extraídos de semillas de frutos inmaduros y la obtención de material para ser empleado en la micropropagación o en programas de restauración ambiental. Se realizó el cultivo de embriones de frutos con seis estadios de crecimiento, hasta un máximo de cuatro semanas. Empleando el medio basal de Murashige & Skoog, se probaron doce medios de cultivo con y sin el agregado de reguladores de crecimiento (ácido giberélico, AG3 y thidiazuron, TDZ). Los embriones se convirtieron en plantas en todos los medios diluidos al 50% (½ MS), manteniendo el 3% de sacarosa, mientras que en el medio adicionado con 10 mg/L de AG3 se obtuvieron plantas con raíces desarrolladas. Si bien el cultivo de embriones derivados de las semillas en los estadios tempranos del fruto no permitió la regeneración de plantas, esta fue posible cuando los explantes derivaron de frutos en estadios tardíos. Instituto de Botánica del Nordeste 2023-08-14 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/bon/article/view/6809 10.30972/bon.3226809 Bonplandia; Vol. 32 No. 2 (2023); 233-244 Bonplandia; Vol. 32 Núm. 2 (2023); 233-244 1853-8460 0524-0476 spa https://revistas.unne.edu.ar/index.php/bon/article/view/6809/6293 Derechos de autor 2023 Bonplandia
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-154
container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Español
format Artículo revista
topic Aquatic plants
growth regulators
Nymphaeaceae
plant regeneration
recalcitrant
Nymphaeaceae
plantas acuáticas
recalcitrantes
regeneración de plantas
reguladores de crecimiento
spellingShingle Aquatic plants
growth regulators
Nymphaeaceae
plant regeneration
recalcitrant
Nymphaeaceae
plantas acuáticas
recalcitrantes
regeneración de plantas
reguladores de crecimiento
Terada, Graciela
Flachsland, Eduardo A.
Medina, Ricardo D.
Mignolli, Francesco
Vidoz, María L.
In vitro culture of embryos isolated from seeds of immature fruits of irupé (Victoria cruziana, Nymphaeaceae)
topic_facet Aquatic plants
growth regulators
Nymphaeaceae
plant regeneration
recalcitrant
Nymphaeaceae
plantas acuáticas
recalcitrantes
regeneración de plantas
reguladores de crecimiento
author Terada, Graciela
Flachsland, Eduardo A.
Medina, Ricardo D.
Mignolli, Francesco
Vidoz, María L.
author_facet Terada, Graciela
Flachsland, Eduardo A.
Medina, Ricardo D.
Mignolli, Francesco
Vidoz, María L.
author_sort Terada, Graciela
title In vitro culture of embryos isolated from seeds of immature fruits of irupé (Victoria cruziana, Nymphaeaceae)
title_short In vitro culture of embryos isolated from seeds of immature fruits of irupé (Victoria cruziana, Nymphaeaceae)
title_full In vitro culture of embryos isolated from seeds of immature fruits of irupé (Victoria cruziana, Nymphaeaceae)
title_fullStr In vitro culture of embryos isolated from seeds of immature fruits of irupé (Victoria cruziana, Nymphaeaceae)
title_full_unstemmed In vitro culture of embryos isolated from seeds of immature fruits of irupé (Victoria cruziana, Nymphaeaceae)
title_sort in vitro culture of embryos isolated from seeds of immature fruits of irupé (victoria cruziana, nymphaeaceae)
description The irupé or water corn (Victoria cruziana A. D. Orb.) belongs to the Nymphaeaceae family and arouses interest mainly due to the beauty of its leaves and flowers. Obtaining seeds of this aquatic species in its natural environment is challenging, and it is also considered a recalcitrant species for in vitro culture. For this reason, the aim of this work was to establish a methodology for the conversion into plants of irupé embryos extracted from seeds of immature fruits and to obtain material to be used in micropropagation or in environmental restoration programs. Embryo culture of fruits in six growth stages was carried out up to a maximum of four weeks. Using Murashige & Skoog’s basal medium, twelve culture media with and without the addition of growth regulators (gibberellic acid, AG3 and thidiazuron, TDZ) were tested. Embryos converted into plants in all media diluted to 50% (½ MS) with 3% sucrose, while plants with developed roots were obtained in the medium with the addition of 10 mg/L of GA3 . Although the culture of embryos derived from seeds in early fruit stages did not allow plant regeneration, this was possible when explants derived from fruits in late developmental stages.
publisher Instituto de Botánica del Nordeste
publishDate 2023
url https://revistas.unne.edu.ar/index.php/bon/article/view/6809
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first_indexed 2024-08-21T22:36:51Z
last_indexed 2024-08-21T22:36:51Z
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