Effect of two dilution temperatures on the quality of semen in cattle from zebu in the Peruvian Tropic
The bull is a factor of genetic and economic importance in cattle breed, since the next generations will depend on it; therefore, the evaluation of the seminal quality and the cryopreservation of its genetic material are essential. The aim of the study was to evaluate the effect of two dilution tem...
Guardado en:
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Universidad Nacional del Nordeste
2023
|
| Materias: | |
| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/6608 |
| Aporte de: |
| id |
I48-R154-article-6608 |
|---|---|
| record_format |
ojs |
| spelling |
I48-R154-article-66082023-06-07T15:15:57Z Effect of two dilution temperatures on the quality of semen in cattle from zebu in the Peruvian Tropic Efecto de dos temperaturas de dilución sobre la calidad de semen en ganado cebuino del Trópico Peruano Segura Portocarrero, G. T. Quispe-Ccasa, H. A. Saucedo-Uriarte, J. A. Poclín Rojas, A. Y. Murga Valderrama, N. L. Cortez Polanco, J. V. Ampuero Trigoso, G. Bull sperm viability cryopreservation morphology seminal freezing Toro viabilidad espermática criopreservación morfología congelación seminal The bull is a factor of genetic and economic importance in cattle breed, since the next generations will depend on it; therefore, the evaluation of the seminal quality and the cryopreservation of its genetic material are essential. The aim of the study was to evaluate the effect of two dilution temperatures on the quality of semen from zebuine cattle from the Peruvian Tropics. The samples were placed in a water bath at 34°C and the macroscopic and microscopic characteristics of semen were analyzed. Only the ejaculates with >60% motility and <20% abnormalities were processed, separating them into two treatments. T1 consisted of a 1:1 predilution at 34°C, followed by a final dilution of 30 minutes by adding the dilutor at 34°C. T2 consisted of a 1:1 predilution at 34°C and after 30 minutes of stabilization, refrigeration was carried out together with the dilutor, in order to carry out the final dilution at 5°C. The seminal quality was analyzed after 16 hours of refrigeration. The percentage of vitality in T1 (80.89%) was greater than in T2 (71.22%) (p<0.05), the positive reaction to the hypoosmotic problem was greater in T1 (71.11%) than in T2 (60%) (p<0.05). Acrosomal integrity and morphometric features did not vary according to treatments. In conclusion, the initial and final dilution at 34°C in Gyr bulls positively influences the semen quality before the freezing of semen packets. El toro es un factor de importancia genética y económica en un hato ganadero, ya que de este dependerán las próximas generaciones; por lo tanto, es esencial la evaluación de la calidad seminal y la criopreservación de su material genético. El objetivo del estudio fue evaluar el efecto de dos temperaturas de dilución sobre la calidad de semen de ganado cebuino del Trópico Peruano. Dos toros Gyr fueron colectados semanalmente mediante electroeyaculación. Las muestras se colocaron en baño maría a 34°C y se analizaron las características macroscópicas y microscópicas del semen. Solo los eyaculados con >60% de motilidad y <20% de anormalidades fueron procesados, separándolos en dos tratamientos. El T1 consistió en predilución 1:1 a 34°C, luego de 30 minutos una dilución final agregando el dilutor a 34°C. El T2 consistió en predilución 1:1 a 34°C y a los 30 minutos de estabilización se llevó a refrigeración conjuntamente con el dilutor, para luego realizar la dilución final a 5°C. La calidad seminal fue analizada luego de 16 horas de refrigeración. El porcentaje de vitalidad en T1 (80,89%) fue mayor que en T2 (71,22%) (p<0,05), la reacción positiva a la prueba hipoosmótica fue mayor en T1 (71,11%) que en T2 (60%) (p<0,05). La integridad acrosomal y los rasgos morfométricos no variaron según tratamientos. En conclusión, la dilución inicial y final a 34°C en toros Gyr influye positivamente sobre la calidad seminal antes de la congelación de pajillas de semen. Universidad Nacional del Nordeste 2023-06-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/6608 10.30972/vet.3416608 Revista Veterinaria; Vol. 34 Núm. 1 (2023); 33-39 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/6608/6148 Derechos de autor 2023 Revista Veterinaria |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-154 |
| container_title_str |
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
Bull sperm viability cryopreservation morphology seminal freezing Toro viabilidad espermática criopreservación morfología congelación seminal |
| spellingShingle |
Bull sperm viability cryopreservation morphology seminal freezing Toro viabilidad espermática criopreservación morfología congelación seminal Segura Portocarrero, G. T. Quispe-Ccasa, H. A. Saucedo-Uriarte, J. A. Poclín Rojas, A. Y. Murga Valderrama, N. L. Cortez Polanco, J. V. Ampuero Trigoso, G. Effect of two dilution temperatures on the quality of semen in cattle from zebu in the Peruvian Tropic |
| topic_facet |
Bull sperm viability cryopreservation morphology seminal freezing Toro viabilidad espermática criopreservación morfología congelación seminal |
| author |
Segura Portocarrero, G. T. Quispe-Ccasa, H. A. Saucedo-Uriarte, J. A. Poclín Rojas, A. Y. Murga Valderrama, N. L. Cortez Polanco, J. V. Ampuero Trigoso, G. |
| author_facet |
Segura Portocarrero, G. T. Quispe-Ccasa, H. A. Saucedo-Uriarte, J. A. Poclín Rojas, A. Y. Murga Valderrama, N. L. Cortez Polanco, J. V. Ampuero Trigoso, G. |
| author_sort |
Segura Portocarrero, G. T. |
| title |
Effect of two dilution temperatures on the quality of semen in cattle from zebu in the Peruvian Tropic |
| title_short |
Effect of two dilution temperatures on the quality of semen in cattle from zebu in the Peruvian Tropic |
| title_full |
Effect of two dilution temperatures on the quality of semen in cattle from zebu in the Peruvian Tropic |
| title_fullStr |
Effect of two dilution temperatures on the quality of semen in cattle from zebu in the Peruvian Tropic |
| title_full_unstemmed |
Effect of two dilution temperatures on the quality of semen in cattle from zebu in the Peruvian Tropic |
| title_sort |
effect of two dilution temperatures on the quality of semen in cattle from zebu in the peruvian tropic |
| description |
The bull is a factor of genetic and economic importance in cattle breed, since the next generations will depend on it; therefore, the evaluation of the seminal quality and the cryopreservation of its genetic material are essential. The aim of the study was to evaluate the effect of two dilution temperatures on the quality of semen from zebuine cattle from the Peruvian Tropics. The samples were placed in a water bath at 34°C and the macroscopic and microscopic characteristics of semen were analyzed. Only the ejaculates with >60% motility and <20% abnormalities were processed, separating them into two treatments. T1 consisted of a 1:1 predilution at 34°C, followed by a final dilution of 30 minutes by adding the dilutor at 34°C. T2 consisted of a 1:1 predilution at 34°C and after 30 minutes of stabilization, refrigeration was carried out together with the dilutor, in order to carry out the final dilution at 5°C. The seminal quality was analyzed after 16 hours of refrigeration. The percentage of vitality in T1 (80.89%) was greater than in T2 (71.22%) (p<0.05), the positive reaction to the hypoosmotic problem was greater in T1 (71.11%) than in T2 (60%) (p<0.05). Acrosomal integrity and morphometric features did not vary according to treatments. In conclusion, the initial and final dilution at 34°C in Gyr bulls positively influences the semen quality before the freezing of semen packets. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2023 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/6608 |
| work_keys_str_mv |
AT seguraportocarrerogt effectoftwodilutiontemperaturesonthequalityofsemenincattlefromzebuintheperuviantropic AT quispeccasaha effectoftwodilutiontemperaturesonthequalityofsemenincattlefromzebuintheperuviantropic AT saucedouriarteja effectoftwodilutiontemperaturesonthequalityofsemenincattlefromzebuintheperuviantropic AT poclinrojasay effectoftwodilutiontemperaturesonthequalityofsemenincattlefromzebuintheperuviantropic AT murgavalderramanl effectoftwodilutiontemperaturesonthequalityofsemenincattlefromzebuintheperuviantropic AT cortezpolancojv effectoftwodilutiontemperaturesonthequalityofsemenincattlefromzebuintheperuviantropic AT ampuerotrigosog effectoftwodilutiontemperaturesonthequalityofsemenincattlefromzebuintheperuviantropic AT seguraportocarrerogt efectodedostemperaturasdedilucionsobrelacalidaddesemenenganadocebuinodeltropicoperuano AT quispeccasaha efectodedostemperaturasdedilucionsobrelacalidaddesemenenganadocebuinodeltropicoperuano AT saucedouriarteja efectodedostemperaturasdedilucionsobrelacalidaddesemenenganadocebuinodeltropicoperuano AT poclinrojasay efectodedostemperaturasdedilucionsobrelacalidaddesemenenganadocebuinodeltropicoperuano AT murgavalderramanl efectodedostemperaturasdedilucionsobrelacalidaddesemenenganadocebuinodeltropicoperuano AT cortezpolancojv efectodedostemperaturasdedilucionsobrelacalidaddesemenenganadocebuinodeltropicoperuano AT ampuerotrigosog efectodedostemperaturasdedilucionsobrelacalidaddesemenenganadocebuinodeltropicoperuano |
| first_indexed |
2024-08-21T22:35:49Z |
| last_indexed |
2024-08-21T22:35:49Z |
| _version_ |
1808038362420871168 |