Preliminary evaluation of the in vivo antigenic power of a new formulation for the bovine babesiosis immunoprophylaxis

Bovine babesiosis is a hemoparasitic disease caused by protozoa of the genus Babesia, the most important are Babesia bovis and B. bigemina, both transmitted by the common bovine tick, Rhipicephalus microplus. In combination with anaplasmosis, they form the Bovine Tristeza Complex, one of the main li...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Álvarez, F. R., Peichoto, M. E., Palma, S., Maletto, B., Lozina, L.
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Nordeste 2022
Materias:
Babesiosis
Immunoprophylaxis
Erythrocytes
Adjuvant
Spray-drying
Inmunoprofilaxis
Eritrocitos
Adyuvante
Secado por aspersión
Acceso en línea:https://revistas.unne.edu.ar/index.php/vet/article/view/6194
Aporte de:
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste
id I48-R154-article-6194
record_format ojs
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-154
container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Español
format Artículo revista
topic Babesiosis
Immunoprophylaxis
Erythrocytes
Adjuvant
Spray-drying
Babesiosis
Inmunoprofilaxis
Eritrocitos
Adyuvante
Secado por aspersión
spellingShingle Babesiosis
Immunoprophylaxis
Erythrocytes
Adjuvant
Spray-drying
Babesiosis
Inmunoprofilaxis
Eritrocitos
Adyuvante
Secado por aspersión
Álvarez, F. R.
Peichoto, M. E.
Palma, S.
Maletto, B.
Lozina, L.
Preliminary evaluation of the in vivo antigenic power of a new formulation for the bovine babesiosis immunoprophylaxis
topic_facet Babesiosis
Immunoprophylaxis
Erythrocytes
Adjuvant
Spray-drying
Babesiosis
Inmunoprofilaxis
Eritrocitos
Adyuvante
Secado por aspersión
author Álvarez, F. R.
Peichoto, M. E.
Palma, S.
Maletto, B.
Lozina, L.
author_facet Álvarez, F. R.
Peichoto, M. E.
Palma, S.
Maletto, B.
Lozina, L.
author_sort Álvarez, F. R.
title Preliminary evaluation of the in vivo antigenic power of a new formulation for the bovine babesiosis immunoprophylaxis
title_short Preliminary evaluation of the in vivo antigenic power of a new formulation for the bovine babesiosis immunoprophylaxis
title_full Preliminary evaluation of the in vivo antigenic power of a new formulation for the bovine babesiosis immunoprophylaxis
title_fullStr Preliminary evaluation of the in vivo antigenic power of a new formulation for the bovine babesiosis immunoprophylaxis
title_full_unstemmed Preliminary evaluation of the in vivo antigenic power of a new formulation for the bovine babesiosis immunoprophylaxis
title_sort preliminary evaluation of the in vivo antigenic power of a new formulation for the bovine babesiosis immunoprophylaxis
description Bovine babesiosis is a hemoparasitic disease caused by protozoa of the genus Babesia, the most important are Babesia bovis and B. bigemina, both transmitted by the common bovine tick, Rhipicephalus microplus. In combination with anaplasmosis, they form the Bovine Tristeza Complex, one of the main limiting factors for productive development in tropical and subtropical areas of the world. Currently, for the prophylaxis of bovine babesiosis in Argentina, only live vaccines are available in fresh and deep-frozen presentations. In the present work, we evaluated the immunogenicity of erythrocytes parasitized with Babesia sp, dehydrated and incorporated into a new adjuvant, formulated for use in humans, as a new immuno prophylactic alternative. With this aim, parasitized erythrocytes obtained from in vitro cultures were dehydrated by spray-dryingtechniques and assembled with an adjuvant. Twelve calves negative for blood parasites wereused, which were divided into 4 groups of 3 animals each one. Control group: without inoculation;Treated Group 1: inoculated with adjuvant; Treated Group 2: adjuvant + erythrocytes parasitized with B. bovis and Treated Group 3: adjuvant + erythrocytes parasitized with B. bigemina. Two subcutaneous inoculations were performed, on 0 and 15 days. During 60 days, rectal temperature, hematocrit and parasitaemia control were performed, in turn, samples for serology were taken on 30- and 60-days post inoculation. The animals remained negative throughout the trial, except on day 60, where 33% of Treated Group 3 presented specific antibody titers against the inoculated agent, B. bigemina. Although the response rate is low, the fact that there is no effective killed vaccine for the prophylaxis of this disease encourages us to continue adjusting the inoculant dose.
publisher Universidad Nacional del Nordeste
publishDate 2022
url https://revistas.unne.edu.ar/index.php/vet/article/view/6194
work_keys_str_mv AT alvarezfr preliminaryevaluationoftheinvivoantigenicpowerofanewformulationforthebovinebabesiosisimmunoprophylaxis
AT peichotome preliminaryevaluationoftheinvivoantigenicpowerofanewformulationforthebovinebabesiosisimmunoprophylaxis
AT palmas preliminaryevaluationoftheinvivoantigenicpowerofanewformulationforthebovinebabesiosisimmunoprophylaxis
AT malettob preliminaryevaluationoftheinvivoantigenicpowerofanewformulationforthebovinebabesiosisimmunoprophylaxis
AT lozinal preliminaryevaluationoftheinvivoantigenicpowerofanewformulationforthebovinebabesiosisimmunoprophylaxis
AT alvarezfr evaluacionpreliminardelpoderantigenicoinvivodeunanuevaformulacionparalainmunoprofilaxisdelababesiosisbovina
AT peichotome evaluacionpreliminardelpoderantigenicoinvivodeunanuevaformulacionparalainmunoprofilaxisdelababesiosisbovina
AT palmas evaluacionpreliminardelpoderantigenicoinvivodeunanuevaformulacionparalainmunoprofilaxisdelababesiosisbovina
AT malettob evaluacionpreliminardelpoderantigenicoinvivodeunanuevaformulacionparalainmunoprofilaxisdelababesiosisbovina
AT lozinal evaluacionpreliminardelpoderantigenicoinvivodeunanuevaformulacionparalainmunoprofilaxisdelababesiosisbovina
first_indexed 2023-03-18T23:07:00Z
last_indexed 2024-08-21T22:35:47Z
_version_ 1808038360507219968
spelling I48-R154-article-61942023-06-23T11:03:41Z Preliminary evaluation of the in vivo antigenic power of a new formulation for the bovine babesiosis immunoprophylaxis Evaluación preliminar del poder antigénico in vivo de una nueva formulación para la inmunoprofilaxis de la babesiosis bovina Álvarez, F. R. Peichoto, M. E. Palma, S. Maletto, B. Lozina, L. Babesiosis Immunoprophylaxis Erythrocytes Adjuvant Spray-drying Babesiosis Inmunoprofilaxis Eritrocitos Adyuvante Secado por aspersión Bovine babesiosis is a hemoparasitic disease caused by protozoa of the genus Babesia, the most important are Babesia bovis and B. bigemina, both transmitted by the common bovine tick, Rhipicephalus microplus. In combination with anaplasmosis, they form the Bovine Tristeza Complex, one of the main limiting factors for productive development in tropical and subtropical areas of the world. Currently, for the prophylaxis of bovine babesiosis in Argentina, only live vaccines are available in fresh and deep-frozen presentations. In the present work, we evaluated the immunogenicity of erythrocytes parasitized with Babesia sp, dehydrated and incorporated into a new adjuvant, formulated for use in humans, as a new immuno prophylactic alternative. With this aim, parasitized erythrocytes obtained from in vitro cultures were dehydrated by spray-dryingtechniques and assembled with an adjuvant. Twelve calves negative for blood parasites wereused, which were divided into 4 groups of 3 animals each one. Control group: without inoculation;Treated Group 1: inoculated with adjuvant; Treated Group 2: adjuvant + erythrocytes parasitized with B. bovis and Treated Group 3: adjuvant + erythrocytes parasitized with B. bigemina. Two subcutaneous inoculations were performed, on 0 and 15 days. During 60 days, rectal temperature, hematocrit and parasitaemia control were performed, in turn, samples for serology were taken on 30- and 60-days post inoculation. The animals remained negative throughout the trial, except on day 60, where 33% of Treated Group 3 presented specific antibody titers against the inoculated agent, B. bigemina. Although the response rate is low, the fact that there is no effective killed vaccine for the prophylaxis of this disease encourages us to continue adjusting the inoculant dose. La babesiosis bovina es una enfermedad hemoparasitaria causada por protozoarios del género Babesia, siendo los de mayor importancia Babesia bovis y B. bigemina, ambos transmitidos por la garrapata común del bovino, Rhipicephalus microplus. Junto con la anaplasmosis, forman el complejo tristeza bovina, uno de los principales factores limitantes para el desarrollo productivo en zonas tropicales y subtropicales del mundo. Actualmente, para la profilaxis de la babesiosis bovina en Argentina solo se dispone de vacunas vivas en las presentaciones, fresca y ultra congelada. En el presente trabajo, se evaluó la inmunogenicidad de eritrocitos parasitados con Babesia sp y deshidratados, incorporados a un nuevo adyuvante, formulado para uso en humanos, como una nueva alternativa inmuno profiláctica. Con tal fin, eritrocitos parasitados obtenidos a partir de cultivos in vitro, fueron deshidratados por técnicas de secado por aspersión y ensamblados con un adyuvante. Se utilizaron 12 terneros negativos a hemoparásitos que fueron distribuidos en 4 grupos de 3 animales cada uno. Grupo Control: sin inocular; Grupo Tratado 1: inoculado con adyuvante; Grupo Tratado 2: adyuvante + eritrocitos parasitados con B. bovis y Grupo Tratado 3: adyuvante + eritrocitos parasitados con B. bigemina. Se realizaron dos inoculaciones subcutáneas, los días 0 y 15. Durante 60 días, se realizó el control de temperatura rectal, hematocrito y parasitemia, así como el muestreo para serología a los 30 y 60 días post-inoculación. Los animales se mantuvieron negativos durante todo el ensayo, excepto al día 60, donde el 33% del Grupo Tratado 3 presentó titulación de anticuerpos contra el agente inoculado, B. bigemina. Si bien el porcentaje de respuesta es bajo, el hecho de no contar a nivel mundial con una vacuna muerta eficaz para la profilaxis de esta enfermedad, nos alienta a continuar ajustando la dosis inoculante. Universidad Nacional del Nordeste 2022-12-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/6194 10.30972/vet.3326194 Revista Veterinaria; Vol. 33 Núm. 2 (2022); 266-272 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/6194/5864 Derechos de autor 2022 Revista Veterinaria

Ejemplares similares