Evaluation of mutagenicity of herbicide glyphosate in the Prochilodus lineatus using the micronucleus test
The use of pesticides can affect the aquatic ecosystem. Through the soil, these pollutants are drained into rivers and lagoons. In Argentina, glyphosate-based agrochemicals such as Roundup are widely used in agricultural activities. Considering this, the objective of this work was to evaluate the ge...
Guardado en:
| Autores principales: | , |
|---|---|
| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Universidad Nacional del Nordeste
2022
|
| Materias: | |
| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/6193 |
| Aporte de: |
| id |
I48-R154-article-6193 |
|---|---|
| record_format |
ojs |
| spelling |
I48-R154-article-61932023-06-21T18:23:57Z Evaluation of mutagenicity of herbicide glyphosate in the Prochilodus lineatus using the micronucleus test Evaluación de la mutagenicidad del herbicida glifosato en Prochilodus lineatus a través del test de micronúcleos Caramello, C. S. Jorge, L. C. Glifosato Micronúcleos Alteraciones morfológicas nucleares Prochilodus lineatus Gliphosate Micronúcleus Nuclear morphology alterations Prochilodus lineatus The use of pesticides can affect the aquatic ecosystem. Through the soil, these pollutants are drained into rivers and lagoons. In Argentina, glyphosate-based agrochemicals such as Roundup are widely used in agricultural activities. Considering this, the objective of this work was to evaluate the genotoxic damage of Roundup Full II® in blood cells of juvenile specimens of Prochilodus lineatus were evaluated. The fish are exposed to concentrations of 0.025 μg/L, 0.05 μg/L and 0.1 μg/L of glyphosate (Roundup Full II) for 7 days. After this period, for the preparation of the smears, blood was extracted from the fish by puncture of the caudal vein. Smear analysis will reveal micronucleus, binucleate cells, lobed nuclei, blebbed nuclei, notched nuclei, nuclear vacuoles, microcytes, buds and cells with nuclear bridge. The statistical analysis showed significant differences between the control and treated groups. In this way, the results suggest the existence of a genetic damage caused by the exposure of the fish to the pesticide. El uso de plaguicidas puede afectar el ecosistema acuático. A través del suelo, estos contaminantes se drenan a los ríos y lagunas. En la Argentina, agroquímicos a base de glifosato como el Roundup, se utilizan ampliamente en actividades agrícolas. Considerando esto, el objetivo de este trabajo fue evaluar los efectos genotóxicos de Roundup Full II® en células sanguíneas de ejemplares juveniles de Prochilodus lineatus. Los peces se expusieron a las concentraciones de 0,025 μg/L, 0,05 μg/L y 0,1 μg/L de glifosato (Roundup Full II) durante 7 días. Después de este período, para la preparación de los frotis se extrajo de los peces sangre por punción de la vena caudal. El análisis de los frotis reveló la ocurrencia de células con micronúcleos, células binucleadas, lobulaciones nucleares (Lobed nuclei; blebbed nuclei), muescas nucleares (notched nuclei), vacuolas nucleares, microcitos, brotes y núcleos con puente. El estudio estadístico mostró diferencias significativas entre los grupos de control y los tratados. De esta manera, los resultados sugieren la existencia de un daño en el material genético causado por la exposición de los peces al pesticida. Universidad Nacional del Nordeste 2022-12-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/6193 10.30972/vet.3326193 Revista Veterinaria; Vol. 33 Núm. 2 (2022); 258-265 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/6193/5863 Derechos de autor 2022 Revista Veterinaria |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-154 |
| container_title_str |
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
Glifosato Micronúcleos Alteraciones morfológicas nucleares Prochilodus lineatus Gliphosate Micronúcleus Nuclear morphology alterations Prochilodus lineatus |
| spellingShingle |
Glifosato Micronúcleos Alteraciones morfológicas nucleares Prochilodus lineatus Gliphosate Micronúcleus Nuclear morphology alterations Prochilodus lineatus Caramello, C. S. Jorge, L. C. Evaluation of mutagenicity of herbicide glyphosate in the Prochilodus lineatus using the micronucleus test |
| topic_facet |
Glifosato Micronúcleos Alteraciones morfológicas nucleares Prochilodus lineatus Gliphosate Micronúcleus Nuclear morphology alterations Prochilodus lineatus |
| author |
Caramello, C. S. Jorge, L. C. |
| author_facet |
Caramello, C. S. Jorge, L. C. |
| author_sort |
Caramello, C. S. |
| title |
Evaluation of mutagenicity of herbicide glyphosate in the Prochilodus lineatus using the micronucleus test |
| title_short |
Evaluation of mutagenicity of herbicide glyphosate in the Prochilodus lineatus using the micronucleus test |
| title_full |
Evaluation of mutagenicity of herbicide glyphosate in the Prochilodus lineatus using the micronucleus test |
| title_fullStr |
Evaluation of mutagenicity of herbicide glyphosate in the Prochilodus lineatus using the micronucleus test |
| title_full_unstemmed |
Evaluation of mutagenicity of herbicide glyphosate in the Prochilodus lineatus using the micronucleus test |
| title_sort |
evaluation of mutagenicity of herbicide glyphosate in the prochilodus lineatus using the micronucleus test |
| description |
The use of pesticides can affect the aquatic ecosystem. Through the soil, these pollutants are drained into rivers and lagoons. In Argentina, glyphosate-based agrochemicals such as Roundup are widely used in agricultural activities. Considering this, the objective of this work was to evaluate the genotoxic damage of Roundup Full II® in blood cells of juvenile specimens of Prochilodus lineatus were evaluated. The fish are exposed to concentrations of 0.025 μg/L, 0.05 μg/L and 0.1 μg/L of glyphosate (Roundup Full II) for 7 days. After this period, for the preparation of the smears, blood was extracted from the fish by puncture of the caudal vein. Smear analysis will reveal micronucleus, binucleate cells, lobed nuclei, blebbed nuclei, notched nuclei, nuclear vacuoles, microcytes, buds and cells with nuclear bridge. The statistical analysis showed significant differences between the control and treated groups. In this way, the results suggest the existence of a genetic damage caused by the exposure of the fish to the pesticide. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2022 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/6193 |
| work_keys_str_mv |
AT caramellocs evaluationofmutagenicityofherbicideglyphosateintheprochiloduslineatususingthemicronucleustest AT jorgelc evaluationofmutagenicityofherbicideglyphosateintheprochiloduslineatususingthemicronucleustest AT caramellocs evaluaciondelamutagenicidaddelherbicidaglifosatoenprochiloduslineatusatravesdeltestdemicronucleos AT jorgelc evaluaciondelamutagenicidaddelherbicidaglifosatoenprochiloduslineatusatravesdeltestdemicronucleos |
| first_indexed |
2023-03-18T23:07:00Z |
| last_indexed |
2024-08-21T22:35:47Z |
| _version_ |
1808038360271290368 |