Relationship between parasitic load and Th1 lymphocytes in skin’s biopsies of dogs with leishmaniasis
Visceral leishmaniasis (VL) is a severe chronic systemic disease caused by Leishmania donovani or L. infantum. Several studies have shown that susceptibility to VL is related to high levels of circulating antibodies and a depression of the immunity mediated by Th1 cells, mainly with decreased produc...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Universidad Nacional del Nordeste
2020
|
| Materias: | |
| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/4610 |
| Aporte de: |
| id |
I48-R154-article-4610 |
|---|---|
| record_format |
ojs |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-154 |
| container_title_str |
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
Dogs leishmaniasis lymphocytes Th1 immunohistochemistry skin biopsy parasitic amount Perros leishmaniosis linfocitos Th1 inmunohistoquímica biopsia de piel carga parasitaria. |
| spellingShingle |
Dogs leishmaniasis lymphocytes Th1 immunohistochemistry skin biopsy parasitic amount Perros leishmaniosis linfocitos Th1 inmunohistoquímica biopsia de piel carga parasitaria. Catuogno, M. S. Burna, A. N. A. Brunel, F. Díaz, B. S. Negrette, M. Relationship between parasitic load and Th1 lymphocytes in skin’s biopsies of dogs with leishmaniasis |
| topic_facet |
Dogs leishmaniasis lymphocytes Th1 immunohistochemistry skin biopsy parasitic amount Perros leishmaniosis linfocitos Th1 inmunohistoquímica biopsia de piel carga parasitaria. |
| author |
Catuogno, M. S. Burna, A. N. A. Brunel, F. Díaz, B. S. Negrette, M. |
| author_facet |
Catuogno, M. S. Burna, A. N. A. Brunel, F. Díaz, B. S. Negrette, M. |
| author_sort |
Catuogno, M. S. |
| title |
Relationship between parasitic load and Th1 lymphocytes in skin’s biopsies of dogs with leishmaniasis |
| title_short |
Relationship between parasitic load and Th1 lymphocytes in skin’s biopsies of dogs with leishmaniasis |
| title_full |
Relationship between parasitic load and Th1 lymphocytes in skin’s biopsies of dogs with leishmaniasis |
| title_fullStr |
Relationship between parasitic load and Th1 lymphocytes in skin’s biopsies of dogs with leishmaniasis |
| title_full_unstemmed |
Relationship between parasitic load and Th1 lymphocytes in skin’s biopsies of dogs with leishmaniasis |
| title_sort |
relationship between parasitic load and th1 lymphocytes in skin’s biopsies of dogs with leishmaniasis |
| description |
Visceral leishmaniasis (VL) is a severe chronic systemic disease caused by Leishmania donovani or L. infantum. Several studies have shown that susceptibility to VL is related to high levels of circulating antibodies and a depression of the immunity mediated by Th1 cells, mainly with decreased production of interferon (IFN-γ) and interleukin-12 (IL-12). Like human leishmaniasis, active canine VL is characterized by polyclonal activation of B-cells and specific immune suppression. Canine patients with reactive serological diagnosis to Leishmania sp were selected and samples were taken for direct parasitological examination and skin biopsies of the pinna for the immune-histochemistry technique, in order to detect Th1 lymphocytes in relation to parasitic amount. In samples of animals without symptoms and high parasite load, the number of Th1 lymphocytes did not exceed 4 cells per field, while in the samples with moderate and low parasite loads, the number of Th1 lymphocytes exceeded 8 cells per field of higher magnification, reaching up to 10. In animals with symptoms, it was observed that in samples with moderate and high parasite loads, the number of Th1 lymphocytes did not exceed 5 cells, while the samples with low parasitic amount exceeded 8 cells per field of higher magnification. It is concluded that the presence or absence of symptoms in canines with VL does not depend on the parasitic amount found in skin biopsies. On the other hand, it was observed that the high parasitic amount is related to immune suppression mediated by Th1 lymphocytes, while in animals with high immunological response, the number of parasites is low. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2020 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/4610 |
| work_keys_str_mv |
AT catuognoms relationshipbetweenparasiticloadandth1lymphocytesinskinsbiopsiesofdogswithleishmaniasis AT burnaan relationshipbetweenparasiticloadandth1lymphocytesinskinsbiopsiesofdogswithleishmaniasis AT abrunelf relationshipbetweenparasiticloadandth1lymphocytesinskinsbiopsiesofdogswithleishmaniasis AT diazb relationshipbetweenparasiticloadandth1lymphocytesinskinsbiopsiesofdogswithleishmaniasis AT snegrettem relationshipbetweenparasiticloadandth1lymphocytesinskinsbiopsiesofdogswithleishmaniasis AT catuognoms relacionentrecargaparasitariaylinfocitosth1enbiopsiasdepieldeperrosconleishmaniosis AT burnaan relacionentrecargaparasitariaylinfocitosth1enbiopsiasdepieldeperrosconleishmaniosis AT abrunelf relacionentrecargaparasitariaylinfocitosth1enbiopsiasdepieldeperrosconleishmaniosis AT diazb relacionentrecargaparasitariaylinfocitosth1enbiopsiasdepieldeperrosconleishmaniosis AT snegrettem relacionentrecargaparasitariaylinfocitosth1enbiopsiasdepieldeperrosconleishmaniosis |
| first_indexed |
2025-05-17T05:09:58Z |
| last_indexed |
2025-05-17T05:09:58Z |
| _version_ |
1832343147834769408 |
| spelling |
I48-R154-article-46102025-02-12T21:59:13Z Relationship between parasitic load and Th1 lymphocytes in skin’s biopsies of dogs with leishmaniasis Relación entre carga parasitaria y linfocitos Th1 en biopsias de piel de perros con leishmaniosis Catuogno, M. S. Burna, A. N. A. Brunel, F. Díaz, B. S. Negrette, M. Dogs leishmaniasis lymphocytes Th1 immunohistochemistry skin biopsy parasitic amount Perros leishmaniosis linfocitos Th1 inmunohistoquímica biopsia de piel carga parasitaria. Visceral leishmaniasis (VL) is a severe chronic systemic disease caused by Leishmania donovani or L. infantum. Several studies have shown that susceptibility to VL is related to high levels of circulating antibodies and a depression of the immunity mediated by Th1 cells, mainly with decreased production of interferon (IFN-γ) and interleukin-12 (IL-12). Like human leishmaniasis, active canine VL is characterized by polyclonal activation of B-cells and specific immune suppression. Canine patients with reactive serological diagnosis to Leishmania sp were selected and samples were taken for direct parasitological examination and skin biopsies of the pinna for the immune-histochemistry technique, in order to detect Th1 lymphocytes in relation to parasitic amount. In samples of animals without symptoms and high parasite load, the number of Th1 lymphocytes did not exceed 4 cells per field, while in the samples with moderate and low parasite loads, the number of Th1 lymphocytes exceeded 8 cells per field of higher magnification, reaching up to 10. In animals with symptoms, it was observed that in samples with moderate and high parasite loads, the number of Th1 lymphocytes did not exceed 5 cells, while the samples with low parasitic amount exceeded 8 cells per field of higher magnification. It is concluded that the presence or absence of symptoms in canines with VL does not depend on the parasitic amount found in skin biopsies. On the other hand, it was observed that the high parasitic amount is related to immune suppression mediated by Th1 lymphocytes, while in animals with high immunological response, the number of parasites is low. La leishmaniosis visceral (LV) es una enfermedad sistémica crónica severa causada por Leishmania donovani o L. infantum. Varios estudios han demostrado que la susceptibilidad a LV está relacionada a altos títulos de anticuerpos circulantes y a una depresión de la inmunidad mediada por linfocitos Th1, principalmente con disminución de la producción de interferon (IFN-γ) e interleukina (IL-12). Similar a la humana, la LV canina está caracterizada por activación policlonal de células B e inmunosupresión específica. El objetivo del trabajo fue establecer la relación entre la carga parasitaria y la respuesta inmune en perros con LV. Se seleccionaron pacientes caninos con diagnóstico serológico reactivo a LV y se les extrajeron muestras para examen parasitológico directo y biopsias de piel del pabellón auricular para efectuar la técnica inmunohistoquímica con el fin de detectar linfocitos Th1 en relación con la carga parasitaria. En muestras de animales sin síntomas que registraban una elevada carga parasitaria, la cantidad de Th1 no superaba las 4 células por campo de mayor aumento, mientras que, en las muestras donde prevalecían cargas moderadas y bajas de parásitos, la cantidad de Th1 superaban las 8 células por campo de mayor aumento, pudiendo llegar hasta 10. De las muestras provenientes de animales con síntomas se observó que en aquéllas con moderada o elevada carga de parásitos, la cantidad de linfocitos Th1 no superaba las 5 células, mientras que las muestras con baja carga parasitaria superaban las 8 células por campo de mayor aumento. Se concluye que la presencia o ausencia de sintomatología en caninos con LV no depende de la carga parasitaria encontrada en las biopsias de piel. Por otra parte, se observó que la alta carga parasitaria se relaciona con inmunosupresión mediada por linfocitos Th1, mientras que en los animales con alta respuesta inmunológica, la carga parasitaria fue baja. Universidad Nacional del Nordeste 2020-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/4610 10.30972/vet.3114610 Revista Veterinaria; Vol. 31 Núm. 1 (2020); 3-7 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/4610/4315 Derechos de autor 2020 M. S. Catuogno, A. N. Burna, F. A. Brunel, B. Díaz, M. S. Negrette https://creativecommons.org/licenses/by-nc/4.0 |