Morphological abnormalities in nucleous of hematies from the fish Prochilodus linneatus exposed to clorpirifos
In recent years, the monitoring of xenobiotics (chemicals such as pesticides) in the environment has been very important, as the persistence of the biological activity of many of these compounds has been recognized. Chlorpyrifos is an organophosphorus insecticide of broad spectrum, used in agricultu...
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| Autores principales: | , , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional del Nordeste
2019
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| Materias: | |
| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/4136 |
| Aporte de: |
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I48-R154-article-4136 |
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| record_format |
ojs |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-154 |
| container_title_str |
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
Fish Prochilodus linneatus chlorpyrifos erythrocytes abnormalities micronucleus Pez Prochilodus linneatus clorpirifos hematíes anormalidades micronúcleos |
| spellingShingle |
Fish Prochilodus linneatus chlorpyrifos erythrocytes abnormalities micronucleus Pez Prochilodus linneatus clorpirifos hematíes anormalidades micronúcleos Caramello, C. S. Cowper, C. F. Jorge, M. J. Pérez, J. E. Jorge, L. C. Morphological abnormalities in nucleous of hematies from the fish Prochilodus linneatus exposed to clorpirifos |
| topic_facet |
Fish Prochilodus linneatus chlorpyrifos erythrocytes abnormalities micronucleus Pez Prochilodus linneatus clorpirifos hematíes anormalidades micronúcleos |
| author |
Caramello, C. S. Cowper, C. F. Jorge, M. J. Pérez, J. E. Jorge, L. C. |
| author_facet |
Caramello, C. S. Cowper, C. F. Jorge, M. J. Pérez, J. E. Jorge, L. C. |
| author_sort |
Caramello, C. S. |
| title |
Morphological abnormalities in nucleous of hematies from the fish Prochilodus linneatus exposed to clorpirifos |
| title_short |
Morphological abnormalities in nucleous of hematies from the fish Prochilodus linneatus exposed to clorpirifos |
| title_full |
Morphological abnormalities in nucleous of hematies from the fish Prochilodus linneatus exposed to clorpirifos |
| title_fullStr |
Morphological abnormalities in nucleous of hematies from the fish Prochilodus linneatus exposed to clorpirifos |
| title_full_unstemmed |
Morphological abnormalities in nucleous of hematies from the fish Prochilodus linneatus exposed to clorpirifos |
| title_sort |
morphological abnormalities in nucleous of hematies from the fish prochilodus linneatus exposed to clorpirifos |
| description |
In recent years, the monitoring of xenobiotics (chemicals such as pesticides) in the environment has been very important, as the persistence of the biological activity of many of these compounds has been recognized. Chlorpyrifos is an organophosphorus insecticide of broad spectrum, used in agricultural and domestic activities. This pesticide as well as other chemicals may accidentally reach the aquatic ecosystem and the ictic fauna. Fish ingesting contaminated substances subsequently develop alterations due to bioaccumulation, becoming effective indicators of environmental pollution. The purpose of this study was to evaluate the genotoxicity of chlorpyrifos in P. linneatus (Pisces, Prochilodontidae) through the frequency of micronuclei (MN) and nuclear morphology alterations (AMN) in peripheral blood erythrocytes. After acclimatization the fish were divided into two control (C) and treated groups (T1 = 10 μg/l, T2 = 30 μg/l and T3 = 90 μg/l). Three replicates per group (C, T) were performed with a total of 3 animals per fishbowl. The animals remained in the aquariums for a period of 7 days. After this time they were sacrificed with an overdose of anaesthetic to extract blood from the caudal vein. Smears were stained with Giemsa. In the samples analyzed, the number of MN and AMN was determined after observation of 2000 cells per animal. Cells with intact cytoplasmic membrane were considered for the study. The analysis of blood smears in both groups revealed the presence of micronuclei and the following nuclear morphology alterations; notches, lobulated, evaginations, eigth shaped, segmentations and vacuolization nuclei. Individuals T3 showed significant variations in the frequency of MN and AMN with respect to the controls (p<0.05), but not the T1 and T2 specimens. We conclude that chlorpyrifos at a dose of 90 μg/l could be harmful to the environment. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2019 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/4136 |
| work_keys_str_mv |
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2025-05-17T05:09:51Z |
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2025-05-17T05:09:51Z |
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I48-R154-article-41362025-02-12T21:59:03Z Morphological abnormalities in nucleous of hematies from the fish Prochilodus linneatus exposed to clorpirifos Anormalidades morfológicas nucleares en hematíes del pez Prochilodus linneatus expuesto al clorpirifos Caramello, C. S. Cowper, C. F. Jorge, M. J. Pérez, J. E. Jorge, L. C. Fish Prochilodus linneatus chlorpyrifos erythrocytes abnormalities micronucleus Pez Prochilodus linneatus clorpirifos hematíes anormalidades micronúcleos In recent years, the monitoring of xenobiotics (chemicals such as pesticides) in the environment has been very important, as the persistence of the biological activity of many of these compounds has been recognized. Chlorpyrifos is an organophosphorus insecticide of broad spectrum, used in agricultural and domestic activities. This pesticide as well as other chemicals may accidentally reach the aquatic ecosystem and the ictic fauna. Fish ingesting contaminated substances subsequently develop alterations due to bioaccumulation, becoming effective indicators of environmental pollution. The purpose of this study was to evaluate the genotoxicity of chlorpyrifos in P. linneatus (Pisces, Prochilodontidae) through the frequency of micronuclei (MN) and nuclear morphology alterations (AMN) in peripheral blood erythrocytes. After acclimatization the fish were divided into two control (C) and treated groups (T1 = 10 μg/l, T2 = 30 μg/l and T3 = 90 μg/l). Three replicates per group (C, T) were performed with a total of 3 animals per fishbowl. The animals remained in the aquariums for a period of 7 days. After this time they were sacrificed with an overdose of anaesthetic to extract blood from the caudal vein. Smears were stained with Giemsa. In the samples analyzed, the number of MN and AMN was determined after observation of 2000 cells per animal. Cells with intact cytoplasmic membrane were considered for the study. The analysis of blood smears in both groups revealed the presence of micronuclei and the following nuclear morphology alterations; notches, lobulated, evaginations, eigth shaped, segmentations and vacuolization nuclei. Individuals T3 showed significant variations in the frequency of MN and AMN with respect to the controls (p<0.05), but not the T1 and T2 specimens. We conclude that chlorpyrifos at a dose of 90 μg/l could be harmful to the environment. Recientemente adquirió importancia el monitoreo de xenobióticos (como pesticidas) en el medio ambiente, debido a la persistencia de la actividad biológica de muchos de ellos. Clorpirifos es un insecticida organofosforado empleado a nivel agrícola y doméstico. Este plaguicida puede alcanzar accidentalmente el ecosistema acuático y la fauna íctica. Al ingerir sustancias contaminadas, los peces desarrollan alteraciones debidas a la bioacumulación, constituyéndose en eficaces indicadores de polución ambiental. El propósito del estudio fue evaluar la genotoxicidad del clorpirifos en P. linneatus (Pisces, Prochilodontidae) a través de la frecuencia de micronúcleos (MN) y de alteraciones de la morfología nuclear (AMN) en eritrocitos de sangre periférica. Los ensayos se realizaron con clorpirifos en su forma pura (Sigma Aldrich). Los peces se dividieron en grupos control (C) y tratados (T1 = 10 μg/l; T2 = 30 μg/l y T3 = 90 μg/l). Se realizaron tres replicas por grupo, con un total de 3 animales por pecera, los cuales permanecieron allí durante 7 días, tras lo cual fueron sacrificados con sobredosis de anestésico. Se extrajo sangre de la vena caudal, realizándose frotis que se colorearon con Giemsa, determinándose el número de MN y AMN luego de la observación de 2000 células por animal. Para el estudio se consideraron las células con sus membranas citoplasmáticas intactas. En ambos grupos, los frotis sanguíneos revelaron la presencia de micronúcleos, así como algunas de las siguientes alteraciones de la morfología nuclear: muesca, lobulación, evaginación, forma de ocho, segmentación y vacuolización nuclear. Los individuos del grupo T3 mostraron variaciones significativas en la frecuencia de MN y AMN respecto a los controles (p<0,05), no así los ejemplares de los tratamientos T1 y T2. Por lo expuesto, surge que el clorpirifos a la dosis de 90 μg/l podría ser nocivo para el medio ambiente. Universidad Nacional del Nordeste 2019-12-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/4136 10.30972/vet.3024136 Revista Veterinaria; Vol. 30 Núm. 2 (2019); 64-72 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/4136/3773 Derechos de autor 2019 C. S. Caramello, C. F. Cowper, M. J. Jorge, J. E. Pérez, L. C. Jorge https://creativecommons.org/licenses/by-nc/4.0 |