Molecular genetic diversity and relationship of indigenous sheep breeds of Pakistan based on nuclear microsatellite loci

Sheep genetic resources are high in Pakistan with a number of different breeds spread throughout the country. However, the molecular diversity of sheep breeds is poorly understood in Pakistan. Therefore, in the present study 16 microsatellite markers were used in microsatellite on Buchi and Hashtnag...

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Detalles Bibliográficos
Autores principales: Hussain, T, Musthafa, M M., Babar, M E., Shaheen, M, Marikar, F M.
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Nordeste 2019
Materias:
Oveja
buchi
hashtnagri
diversidad genética
marcadores microsatelitales
Pakistán
Sheep
genetic diversity
microsatellite markers
Acceso en línea:https://revistas.unne.edu.ar/index.php/vet/article/view/3906
Aporte de:
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste
id I48-R154-article-3906
record_format ojs
spelling I48-R154-article-39062025-02-12T21:58:58Z Molecular genetic diversity and relationship of indigenous sheep breeds of Pakistan based on nuclear microsatellite loci Diversidad genética molecular y relación entre ovejas de razas autóctonas de Pakistan basada en locus microsatelitales nucleares Hussain, T Musthafa, M M. Babar, M E. Shaheen, M Marikar, F M. Oveja buchi hashtnagri diversidad genética marcadores microsatelitales Pakistán Sheep buchi hashtnagri genetic diversity microsatellite markers Pakistán Sheep genetic resources are high in Pakistan with a number of different breeds spread throughout the country. However, the molecular diversity of sheep breeds is poorly understood in Pakistan. Therefore, in the present study 16 microsatellite markers were used in microsatellite on Buchi and Hashtnagri indigenous breeds of Punjab and Balochistan provinces, respectively. Blood samples from 25 unrelated individuals were collected for genetic diversity and relationship investigation. The mean number of alleles on Buchi and Hashtnagri were 3.375±1.455 and 3.50±1.591, respectively. The mean observed heterozygosity for Buchi was 0.878±0.204 while for Hashtnagri it was 0.885±0.218. The mean Shannon Index showed 1.032±0.371 and 1.070±0.412 for Buchi and Hashtnagri respectively. Inbreeding estimates (FIS and FIT) showed negative values while mean gene flow showed 10.09 and mean population difference (FST) showed 2.4%. According to these results, Buchi and Hashtnagri indigenous breeds showed considerable amount of genetic diversity. There is a decent scope for conservation, effective improvement, and designing suitable breeding strategies for sheep breeds in near future. En Pakistán los recursos genéticos ovinos son abundantes, disponiéndose de varias diferentes castas extendidas en todo el país. No obstante, la diversidad molecular de las razas de ovejas es poco conocida en la región. Por ello, en el presente estudio se investigaron 16 marcadores microsatelitales de las razas indígenas Buchi y Hashtnagri, en las provincias de Pendjab y Balochistan respectivamente. Para indagar la diversidad genética se obtuvieron muestras de sangre de 25 animales de cada raza. El promedio del número de alelos en Buchi y Hashtnagri fue de 3,375±1,455 y 3,50±1,591 respectivamente. Los promedios de heterocigosis registradas fueron de 0,878±0,204 para la raza Buchi y 0,885±0,218 para Hashtnagri. Los índices de Shannon fueron 1,032±0,371 para Buchi y 1,070±0,412 para Hashtnagri, respectivamente. La estimación de los coeficientes de endogamia (FIS y FIT) mostró valores negativos, en tanto que el flujo genético (migración) fue de 10,09 y el promedio de la diferencia poblacional (FST) resultó de 2,4%. En ambas razas, el índice de información polimórfica fue 0,56, indicando el valor del marcador del tablero. Entre las ovejas Buchi y Hashtnagri, la distancia genética estándar de Nei (Ds) fue 0,0218. Según estos resultados, ambas razas mostraron considerable diversidad genética. Los datos obtenidos permiten vislumbrar una promisoria mejoría en el área de la conservación y en el diseño de las estrategias para la cría de ovejas en el futuro cercano. Universidad Nacional del Nordeste 2019-08-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/3906 10.30972/vet.3013906 Revista Veterinaria; Vol. 30 Núm. 1 (2019); 54-58 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/3906/3533 Derechos de autor 2019 T Hussain, M M. Musthafa, M E. Babar, M Shaheen, F M. Marikar https://creativecommons.org/licenses/by-nc/4.0
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-154
container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Español
format Artículo revista
topic Oveja
buchi
hashtnagri
diversidad genética
marcadores microsatelitales
Pakistán
Sheep
buchi
hashtnagri
genetic diversity
microsatellite markers
Pakistán
spellingShingle Oveja
buchi
hashtnagri
diversidad genética
marcadores microsatelitales
Pakistán
Sheep
buchi
hashtnagri
genetic diversity
microsatellite markers
Pakistán
Hussain, T
Musthafa, M M.
Babar, M E.
Shaheen, M
Marikar, F M.
Molecular genetic diversity and relationship of indigenous sheep breeds of Pakistan based on nuclear microsatellite loci
topic_facet Oveja
buchi
hashtnagri
diversidad genética
marcadores microsatelitales
Pakistán
Sheep
buchi
hashtnagri
genetic diversity
microsatellite markers
Pakistán
author Hussain, T
Musthafa, M M.
Babar, M E.
Shaheen, M
Marikar, F M.
author_facet Hussain, T
Musthafa, M M.
Babar, M E.
Shaheen, M
Marikar, F M.
author_sort Hussain, T
title Molecular genetic diversity and relationship of indigenous sheep breeds of Pakistan based on nuclear microsatellite loci
title_short Molecular genetic diversity and relationship of indigenous sheep breeds of Pakistan based on nuclear microsatellite loci
title_full Molecular genetic diversity and relationship of indigenous sheep breeds of Pakistan based on nuclear microsatellite loci
title_fullStr Molecular genetic diversity and relationship of indigenous sheep breeds of Pakistan based on nuclear microsatellite loci
title_full_unstemmed Molecular genetic diversity and relationship of indigenous sheep breeds of Pakistan based on nuclear microsatellite loci
title_sort molecular genetic diversity and relationship of indigenous sheep breeds of pakistan based on nuclear microsatellite loci
description Sheep genetic resources are high in Pakistan with a number of different breeds spread throughout the country. However, the molecular diversity of sheep breeds is poorly understood in Pakistan. Therefore, in the present study 16 microsatellite markers were used in microsatellite on Buchi and Hashtnagri indigenous breeds of Punjab and Balochistan provinces, respectively. Blood samples from 25 unrelated individuals were collected for genetic diversity and relationship investigation. The mean number of alleles on Buchi and Hashtnagri were 3.375±1.455 and 3.50±1.591, respectively. The mean observed heterozygosity for Buchi was 0.878±0.204 while for Hashtnagri it was 0.885±0.218. The mean Shannon Index showed 1.032±0.371 and 1.070±0.412 for Buchi and Hashtnagri respectively. Inbreeding estimates (FIS and FIT) showed negative values while mean gene flow showed 10.09 and mean population difference (FST) showed 2.4%. According to these results, Buchi and Hashtnagri indigenous breeds showed considerable amount of genetic diversity. There is a decent scope for conservation, effective improvement, and designing suitable breeding strategies for sheep breeds in near future.
publisher Universidad Nacional del Nordeste
publishDate 2019
url https://revistas.unne.edu.ar/index.php/vet/article/view/3906
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first_indexed 2025-05-17T05:09:46Z
last_indexed 2025-05-17T05:09:46Z
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