Direct fluorescent antibody test and bacteriological culture for detection of Brucella suis in swine tissues
Methods available for detection of Brucella sp from different specimens include bacteriological culture or detection of specific DNA fragments by polymerase chain reaction. The use of fluorescein-labeled anti-Brucella globulin for demonstrating this antigen in animal tissues is a simple, easy, repro...
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional del Nordeste
2019
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| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/3896 |
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I48-R154-article-38962025-02-12T21:58:57Z Direct fluorescent antibody test and bacteriological culture for detection of Brucella suis in swine tissues Comparación del test directo de anticuerpos fluorescentes y el cultivo bacteriológico para detección de Brucella suis Estein, S M. Bence, A R. Cacciato, C S. Echavarría, H M. Soto, P Cerdo Brucella sp fluoresceína anti-Brucella globulina impronta sensibilidad especificidad Swine Brucella sp fluorescein labeled anti Brucella globulin smear sensitivity specificity Methods available for detection of Brucella sp from different specimens include bacteriological culture or detection of specific DNA fragments by polymerase chain reaction. The use of fluorescein-labeled anti-Brucella globulin for demonstrating this antigen in animal tissues is a simple, easy, reproducible, cheap and fast technique. The aim of this work was to evaluate the gamma globulin fraction of polyclonal anti-Brucella abortus serum labeled with fluorescein iso-tio-cyanate (FITC-labeled antibody): 1) against different smooth and rough Brucella sp, 2) against bacterium of other genus, and 3) to compare direct fluorescent antibody test results with bacteriological culture for the detection of B. suis in different tissues from infected animals. This conjugate stained all Brucella sp with different intensities but it did not stain any heterologous bacterium tested. Background fluorescence associated with its use on smears from infected sources of different specimens was particularly low. Most of the infected tissues showed the presence of yellowish-green fluorescent organisms with brucella morphology. The tested FITC-labeled antibody allows a quick, effective and inexpensive diagnosis of brucellosis. El diagnóstico de brucelosis se apoya en el cultivo bacteriológico o en la detección de fragmentos de ADN de la bacteria mediante la reacción en cadena de la polimerasa. El empleo de una inmunoglobulina anti-Brucella conjugada a fluoresceína para la detección de este antígeno en tejidos constituye una técnica simple, fácil, reproducible, económica y rápida. El objetivo de este trabajo fue evaluar la fracción gammaglobulínica de un suero policlonal anti-Brucella abortus marcada con isotiocianato de fluoresceína (FITC), 1) contra distintas especies lisas y rugosas de Brucella sp, 2) contra bacterias de otros géneros, y 3) comparar los resultados obtenidos con la inmunofluorescencia directa y el cultivo bacteriológico para la detección de B. suis en distintos tejidos de porcinos infectados. Este conjugado detectó todas las brucelas con distinta intensidad de fluorescencia, pero no hubo fluorescencia inespecífica cuando se ensayaron las bacterias de otros géneros. La fluorescencia de fondo en muestras de los distintos tejidos infectados fue baja. La mayoría de los tejidos infectados mostraron la presencia de microorganismos verde-fluorescentes con la morfología de las brucelas. El anticuerpo conjugado a FITC permitió un diagnóstico de brucelosis rápido, efectivo y económico. Universidad Nacional del Nordeste 2019-08-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/3896 10.30972/vet.3013896 Revista Veterinaria; Vol. 30 Núm. 1 (2019); 39-42 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/3896/3517 Derechos de autor 2019 S M. Estein, A R. Bence, C S. Cacciato, H M. Echavarría, P Soto https://creativecommons.org/licenses/by-nc/4.0 |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-154 |
| container_title_str |
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
Cerdo Brucella sp fluoresceína anti-Brucella globulina impronta sensibilidad especificidad Swine Brucella sp fluorescein labeled anti Brucella globulin smear sensitivity specificity |
| spellingShingle |
Cerdo Brucella sp fluoresceína anti-Brucella globulina impronta sensibilidad especificidad Swine Brucella sp fluorescein labeled anti Brucella globulin smear sensitivity specificity Estein, S M. Bence, A R. Cacciato, C S. Echavarría, H M. Soto, P Direct fluorescent antibody test and bacteriological culture for detection of Brucella suis in swine tissues |
| topic_facet |
Cerdo Brucella sp fluoresceína anti-Brucella globulina impronta sensibilidad especificidad Swine Brucella sp fluorescein labeled anti Brucella globulin smear sensitivity specificity |
| author |
Estein, S M. Bence, A R. Cacciato, C S. Echavarría, H M. Soto, P |
| author_facet |
Estein, S M. Bence, A R. Cacciato, C S. Echavarría, H M. Soto, P |
| author_sort |
Estein, S M. |
| title |
Direct fluorescent antibody test and bacteriological culture for detection of Brucella suis in swine tissues |
| title_short |
Direct fluorescent antibody test and bacteriological culture for detection of Brucella suis in swine tissues |
| title_full |
Direct fluorescent antibody test and bacteriological culture for detection of Brucella suis in swine tissues |
| title_fullStr |
Direct fluorescent antibody test and bacteriological culture for detection of Brucella suis in swine tissues |
| title_full_unstemmed |
Direct fluorescent antibody test and bacteriological culture for detection of Brucella suis in swine tissues |
| title_sort |
direct fluorescent antibody test and bacteriological culture for detection of brucella suis in swine tissues |
| description |
Methods available for detection of Brucella sp from different specimens include bacteriological culture or detection of specific DNA fragments by polymerase chain reaction. The use of fluorescein-labeled anti-Brucella globulin for demonstrating this antigen in animal tissues is a simple, easy, reproducible, cheap and fast technique. The aim of this work was to evaluate the gamma globulin fraction of polyclonal anti-Brucella abortus serum labeled with fluorescein iso-tio-cyanate (FITC-labeled antibody): 1) against different smooth and rough Brucella sp, 2) against bacterium of other genus, and 3) to compare direct fluorescent antibody test results with bacteriological culture for the detection of B. suis in different tissues from infected animals. This conjugate stained all Brucella sp with different intensities but it did not stain any heterologous bacterium tested. Background fluorescence associated with its use on smears from infected sources of different specimens was particularly low. Most of the infected tissues showed the presence of yellowish-green fluorescent organisms with brucella morphology. The tested FITC-labeled antibody allows a quick, effective and inexpensive diagnosis of brucellosis. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2019 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/3896 |
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