Protective effect of vitamin C on oxidative stress and DNA damage in rats with diabetes mellitus

Diabetes mellitus (DM) is a metabolic disease of endocrine origin, which main biochemical characteristic is chronic hyperglycemia. The aim of this study was to measure the protective effect of vitamin C (VitC) on oxidative stress in Sprague-Dawley rats with DM induced by Streptozotocin (STZ). We use...

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Autores principales: Mendoza, C, Flores, C, Melendez, C, Marquez, Y C., Matheus, N
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Nordeste 2019
Materias:
Rat
induced diabetes mellitus
vitamin C
ADN damage
Rata
diabetes
mellitus inducida
vitamina C
ADN
Acceso en línea:https://revistas.unne.edu.ar/index.php/vet/article/view/3891
Aporte de:
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste
id I48-R154-article-3891
record_format ojs
spelling I48-R154-article-38912025-02-12T21:58:55Z Protective effect of vitamin C on oxidative stress and DNA damage in rats with diabetes mellitus Efecto protector de la vitamina C sobre el estrés oxidativo y daño al ADN en ratas con diabetes mellitus Mendoza, C Flores, C Melendez, C Marquez, Y C. Matheus, N Rat induced diabetes mellitus vitamin C ADN damage Rata diabetes mellitus inducida vitamina C ADN Diabetes mellitus (DM) is a metabolic disease of endocrine origin, which main biochemical characteristic is chronic hyperglycemia. The aim of this study was to measure the protective effect of vitamin C (VitC) on oxidative stress in Sprague-Dawley rats with DM induced by Streptozotocin (STZ). We used 16 rats (100 ± 20g), divided into 4 groups: 1) control, 2) STZ (diabetics), 3) VitC + STZ, 4) VitC. In liver homogenate, the concentration of malondiahaldehido, conjugated dienes, and total proteins were determined, as well as the activity of superoxide dismutase and catalase. The ADN damage was measured by comet assay. The results were statistically analyzed using SPSS software version 17.0 for Windows. An Anova test and a multiple DMS comparison (p<0.05) were applied. In the group of animals treated with VitC a decrease in glucose levels was evidenced, as well as in the generation of oxidative stress. Less ADN damage induced by free radicals was also determined. It can be infered that VitC is an effective antioxidant and is a liver protector against to the permanent production of free radicals by diverse sources and that it is also effective in the repair and conservation of ADN.  La diabetes mellitas (DM) es una enfermedad metabólica de origen endocrino, cuya principal característica bioquímica es la hiperglucemia crónica. Desencadena procesos bioquímicos graves para el organismo como el estrés oxidativo (EO). El objetivo de este estudio fue evaluar el efecto protector de la vitamina C (VitC) sobre el EO en ratas Sprague-Dawley con DM inducida por Streptozotocina (STZ). Se utilizaron 16 ratas (100 ± 20 g), divididas en 4 grupos: 1) control, 2) STZ (diabéticas), 3) VitC + STZ, 4) VitC. En homogenizado de hígado se determinó la concentración de malondiahaldehido, dienos conjugados y proteínas totales. Por otra parte se determinaron las actividades de superóxido dismutasa y catalasa. También se midió el daño al ADN hepático por ensayo cometa. Los resultados se analizaron estadísticamente mediante programa SPSS versión 17.0 para Windows. Se aplicó un Anova y una prueba de comparación múltiple DMS (p<0,05). Se pudo evidenciar que en el grupo de animales tratados con VitC disminuyeron los niveles de glucosa y la generación de EO; también hubo menor daño del ADN por radicales libres. Estos datos permiten inferir que la VitC ejerce una efectiva acción antioxidante y protectora del hígado ante la permanente producción de radicales libres; además de ser efectiva en la reparación y conservación del ADN.  Universidad Nacional del Nordeste 2019-08-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/3891 10.30972/vet.3013891 Revista Veterinaria; Vol. 30 Núm. 1 (2019); 12-16 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/3891/3512 Derechos de autor 2019 C Mendoza, C Flores, C Melendez, Y C. Marquez, N Matheus https://creativecommons.org/licenses/by-nc/4.0
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-154
container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Español
format Artículo revista
topic Rat
induced diabetes mellitus
vitamin C
ADN damage
Rata
diabetes
mellitus inducida
vitamina C
ADN
spellingShingle Rat
induced diabetes mellitus
vitamin C
ADN damage
Rata
diabetes
mellitus inducida
vitamina C
ADN
Mendoza, C
Flores, C
Melendez, C
Marquez, Y C.
Matheus, N
Protective effect of vitamin C on oxidative stress and DNA damage in rats with diabetes mellitus
topic_facet Rat
induced diabetes mellitus
vitamin C
ADN damage
Rata
diabetes
mellitus inducida
vitamina C
ADN
author Mendoza, C
Flores, C
Melendez, C
Marquez, Y C.
Matheus, N
author_facet Mendoza, C
Flores, C
Melendez, C
Marquez, Y C.
Matheus, N
author_sort Mendoza, C
title Protective effect of vitamin C on oxidative stress and DNA damage in rats with diabetes mellitus
title_short Protective effect of vitamin C on oxidative stress and DNA damage in rats with diabetes mellitus
title_full Protective effect of vitamin C on oxidative stress and DNA damage in rats with diabetes mellitus
title_fullStr Protective effect of vitamin C on oxidative stress and DNA damage in rats with diabetes mellitus
title_full_unstemmed Protective effect of vitamin C on oxidative stress and DNA damage in rats with diabetes mellitus
title_sort protective effect of vitamin c on oxidative stress and dna damage in rats with diabetes mellitus
description Diabetes mellitus (DM) is a metabolic disease of endocrine origin, which main biochemical characteristic is chronic hyperglycemia. The aim of this study was to measure the protective effect of vitamin C (VitC) on oxidative stress in Sprague-Dawley rats with DM induced by Streptozotocin (STZ). We used 16 rats (100 ± 20g), divided into 4 groups: 1) control, 2) STZ (diabetics), 3) VitC + STZ, 4) VitC. In liver homogenate, the concentration of malondiahaldehido, conjugated dienes, and total proteins were determined, as well as the activity of superoxide dismutase and catalase. The ADN damage was measured by comet assay. The results were statistically analyzed using SPSS software version 17.0 for Windows. An Anova test and a multiple DMS comparison (p<0.05) were applied. In the group of animals treated with VitC a decrease in glucose levels was evidenced, as well as in the generation of oxidative stress. Less ADN damage induced by free radicals was also determined. It can be infered that VitC is an effective antioxidant and is a liver protector against to the permanent production of free radicals by diverse sources and that it is also effective in the repair and conservation of ADN. 
publisher Universidad Nacional del Nordeste
publishDate 2019
url https://revistas.unne.edu.ar/index.php/vet/article/view/3891
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first_indexed 2025-05-17T05:09:43Z
last_indexed 2025-05-17T05:09:43Z
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