Antioxidant effect of lutein protects against oxidative damage to porcine spermatozoa
<p Boar sperm is especially susceptible to peroxidative damage generated by reactive oxygen species (ROS). Chemiluminescence was initiated by incubating porcine semen in an in vitro ascorbate-Fe++ system, a technique that allows the evaluation of oxidative stress in these cells. Lutein is kno...
| Autores principales: | , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Universidad Nacional del Nordeste
2019
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| Materias: | |
| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/3876 |
| Aporte de: |
| id |
I48-R154-article-3876 |
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| record_format |
ojs |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-154 |
| container_title_str |
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
Porcine spermatozoa reactives oxygen species chemiluminescence lutein Porcine spermatozoa reactives oxygen species chemiluminescence lutein |
| spellingShingle |
Porcine spermatozoa reactives oxygen species chemiluminescence lutein Porcine spermatozoa reactives oxygen species chemiluminescence lutein Gavazza, M Marmunti, M Compagnoni, M Palacios, A Antioxidant effect of lutein protects against oxidative damage to porcine spermatozoa |
| topic_facet |
Porcine spermatozoa reactives oxygen species chemiluminescence lutein Porcine spermatozoa reactives oxygen species chemiluminescence lutein |
| author |
Gavazza, M Marmunti, M Compagnoni, M Palacios, A |
| author_facet |
Gavazza, M Marmunti, M Compagnoni, M Palacios, A |
| author_sort |
Gavazza, M |
| title |
Antioxidant effect of lutein protects against oxidative damage to porcine spermatozoa |
| title_short |
Antioxidant effect of lutein protects against oxidative damage to porcine spermatozoa |
| title_full |
Antioxidant effect of lutein protects against oxidative damage to porcine spermatozoa |
| title_fullStr |
Antioxidant effect of lutein protects against oxidative damage to porcine spermatozoa |
| title_full_unstemmed |
Antioxidant effect of lutein protects against oxidative damage to porcine spermatozoa |
| title_sort |
antioxidant effect of lutein protects against oxidative damage to porcine spermatozoa |
| description |
<p Boar sperm is especially susceptible to peroxidative damage generated by reactive oxygen species (ROS). Chemiluminescence was initiated by incubating porcine semen in an in vitro ascorbate-Fe++ system, a technique that allows the evaluation of oxidative stress in these cells. Lutein is known for its antioxidant effects, chemically it is a dihydric derivative of α-carotene and belongs to the group of xanthophylls. The main objective of this study was to investigate the antioxidant effect of lutein on boar spermatozoa. The effect of lutein was analyzed by two methods: 1) by adding lutein: the sperm samples were placed in an in vitro ascorbate- Fe++ system, during 120 min at 37°C adding increasing amounts of lutein (50, 150 and 250 μg) per mg of protein and 2) by incubation with lutein in an in vitro ascorbate-Fe+2 system, for 120 min at 37°C, using spermatozoa obtained from porcine semen samples previously incubated with lutein (0.15 and 0.25 mg/ml) during 24 h at 15°C. In both methods a control group (without lutein) was used. Peroxidation was measured by chemiluminescence using a liquid scintillation counter, the light emission being quantified in cpm (counts per minute). Analyzing the effect of lutein by the two methods, it was observed that the total amount of cpm/mg of protein originated by chemiluminescence was lower in samples obtained from the lutein group than in the control group (without lutein). Total chemiluminescence (cpm total) was lower in samples obtained from the lutein group than in the control group (without lutein), with a significance of p<0.005. Percent inhibition of peroxidation was not concentration dependent. These results would demonstrate that lutein could act as an antioxidant that would protect the membranes of the sperm from oxidative damage.p> |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2019 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/3876 |
| work_keys_str_mv |
AT gavazzam antioxidanteffectofluteinprotectsagainstoxidativedamagetoporcinespermatozoa AT marmuntim antioxidanteffectofluteinprotectsagainstoxidativedamagetoporcinespermatozoa AT compagnonim antioxidanteffectofluteinprotectsagainstoxidativedamagetoporcinespermatozoa AT palaciosa antioxidanteffectofluteinprotectsagainstoxidativedamagetoporcinespermatozoa |
| first_indexed |
2025-05-17T05:09:43Z |
| last_indexed |
2025-05-17T05:09:43Z |
| _version_ |
1832343131755905024 |
| spelling |
I48-R154-article-38762025-02-12T21:58:54Z Antioxidant effect of lutein protects against oxidative damage to porcine spermatozoa Antioxidant effect of lutein protects against oxidative damage to porcine spermatozoa Gavazza, M Marmunti, M Compagnoni, M Palacios, A Porcine spermatozoa reactives oxygen species chemiluminescence lutein Porcine spermatozoa reactives oxygen species chemiluminescence lutein <p Boar sperm is especially susceptible to peroxidative damage generated by reactive oxygen species (ROS). Chemiluminescence was initiated by incubating porcine semen in an in vitro ascorbate-Fe++ system, a technique that allows the evaluation of oxidative stress in these cells. Lutein is known for its antioxidant effects, chemically it is a dihydric derivative of α-carotene and belongs to the group of xanthophylls. The main objective of this study was to investigate the antioxidant effect of lutein on boar spermatozoa. The effect of lutein was analyzed by two methods: 1) by adding lutein: the sperm samples were placed in an in vitro ascorbate- Fe++ system, during 120 min at 37°C adding increasing amounts of lutein (50, 150 and 250 μg) per mg of protein and 2) by incubation with lutein in an in vitro ascorbate-Fe+2 system, for 120 min at 37°C, using spermatozoa obtained from porcine semen samples previously incubated with lutein (0.15 and 0.25 mg/ml) during 24 h at 15°C. In both methods a control group (without lutein) was used. Peroxidation was measured by chemiluminescence using a liquid scintillation counter, the light emission being quantified in cpm (counts per minute). Analyzing the effect of lutein by the two methods, it was observed that the total amount of cpm/mg of protein originated by chemiluminescence was lower in samples obtained from the lutein group than in the control group (without lutein). Total chemiluminescence (cpm total) was lower in samples obtained from the lutein group than in the control group (without lutein), with a significance of p<0.005. Percent inhibition of peroxidation was not concentration dependent. These results would demonstrate that lutein could act as an antioxidant that would protect the membranes of the sperm from oxidative damage.p> Boar sperm is especially susceptible to peroxidative damage generated by reactive oxygen species (ROS). Chemiluminescence was initiated by incubating porcine semen in an in vitro ascorbate-Fe++ system, a technique that allows the evaluation of oxidative stress in these cells. Lutein is known for its antioxidant effects, chemically it is a dihydric derivative of α-carotene and belongs to the group of xanthophylls. The main objective of this study was to investigate the antioxidant effect of lutein on boar spermatozoa. The effect of lutein was analyzed by two methods: 1) by adding lutein: the sperm samples were placed in an in vitro ascorbate-Fe++ system, during 120 min at 37°C adding increasing amounts of lutein (50, 150 and 250 μg) per mg of protein and 2) by incubation with lutein in an in vitro ascorbate-Fe+2 system, for 120 min at 37°C, using spermatozoa obtained from porcine semen samples previously incubated with lutein (0.15 and 0.25 mg/ml) during 24 h at 15°C. In both methods a control group (without lutein) was used. Peroxidation was measured by chemiluminescence using a liquid scintillation counter, the light emission being quantified in cpm (counts per minute). Analyzing the effect of lutein by the two methods, it was observed that the total amount of cpm/mg of protein originated by chemiluminescence was lower in samples obtained from the lutein group than in the control group (without lutein). Total chemiluminescence (cpm total) was lower in samples obtained from the lutein group than in the control group (without lutein), with a significance of p<0.005. Percent inhibition of peroxidation was not concentration dependent. These results would demonstrate that lutein could act as an antioxidant that would protect the membranes of the sperm from oxidative damage. Universidad Nacional del Nordeste 2019-08-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/3876 10.30972/vet.3013876 Revista Veterinaria; Vol. 30 Núm. 1 (2019); 7-11 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/3876/3494 Derechos de autor 2019 M Gavazza, M Marmunti, M Compagnoni, A Palacios https://creativecommons.org/licenses/by-nc/4.0 |