Effect of royal jelly on bovine sperm characteristics during post–thaw incubation in vitro

Cryopreserved bovine semen is generally considered to have lower fertility compared to fresh semen. The reduction arises from both a lower post–thaw viability and a possible sublethal dysfunction of the surviving sperm population. The present study was conducted to observe the effect of royal jelly...

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Autor principal: Abd–Allah, Saber Mohammed
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Nordeste 2010
Materias:
bovino
semen
jalea real
longevidad
viabilidad
cattle
sperm
royal jelly
longevity
viability
Acceso en línea:https://revistas.unne.edu.ar/index.php/vet/article/view/1747
Aporte de:
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste
id I48-R154-article-1747
record_format ojs
spelling I48-R154-article-17472025-02-12T21:57:28Z Effect of royal jelly on bovine sperm characteristics during post–thaw incubation in vitro Efectos de la incubación in vitro con jalea real sobre las características del semen bovino descongelado Abd–Allah, Saber Mohammed bovino semen jalea real longevidad viabilidad cattle sperm royal jelly longevity viability Cryopreserved bovine semen is generally considered to have lower fertility compared to fresh semen. The reduction arises from both a lower post–thaw viability and a possible sublethal dysfunction of the surviving sperm population. The present study was conducted to observe the effect of royal jelly (RJ) on motility, viability and acrosome integrity of spermatozoa during post–thaw incubation. Frozen–thawed semen samples were washed and incubated at 37°C in Tris buffer containing 0.1, 0.2, 0.3, 0.4, 0.5% RJ or none (control). Sperm motility, viability and acrosomal integrity were assessed at 0, 0.5, 1, 1.5 and 2 h. The percentages of sperm motility, viability and intact acrosome were higher in Tris buffer containing 0.4% RJ compared to control (p < 0.05). After 2 h of incubation the percentages of motility, viability and intact acrosome of spermatozoa, respectively, were 52.3; 52.5 and 19.8% in 0.4% RJ containing buffer. Results indicate that the addition of 0.4% RJ in the incubation media was able to maintain better quality and longevity of spermatozoa. Royal jelly may be used as a semen extender to improve sperm quality and fertility. El semen bovino criopreservado generalmente es considerado de menor poder fertilizante comparado con el semen fresco. Tal reducción abarca tanto una más baja viabilidad post–descongelado como una posible disfunción subletal de la población de espermatozoides sobrevivientes. El presente estudio fue realizado para determinar el efecto de la jalea real (JR) sobre la motilidad, viabilidad e integridad acrosomal de espermatozoides durante la etapa de incubación post–descongelado. Las muestras de semen congelado–descongelado se lavaron e incubaron a 37°C en buffer Tris conteniendo JR en proporción de 0,1; 0,2; 0,3; 0,4; 0,5% o ninguna (control). La motilidad y la viabilidad espermáticas, así como la integridad acrosomal, fueron evaluadas a las 0; 0,5; 1; 1,5 y 2 h. Comparados con los controles, los porcentajes de motilidad, viabilidad e integridad acrosomal fueron más altos en los espermatozoides incubados en Tris conteniendo 0,4% de JR (p < 0,05). Después de 2 h de incubación, los porcentajes de motilidad, viabilidad e integridad acrosomal de los espermatozoides fueron respectivamente de 52,3; 52,5 y 19,8% en el buffer que contenía JR al 0,4%. Los resultados indican que la adición de 0,4% de JR en los medios de incubación fue capaz de mantener la buena calidad y longevidad de los espermatozoides. La jalea real puede ser usada como aditivo seminal para mejorar la viabilidad y fertilidad de los espermatozoides. Universidad Nacional del Nordeste 2010-07-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/1747 10.30972/vet.2121747 Revista Veterinaria; Vol. 21 Núm. 2 (2010); 81-85 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/1747/1505 Derechos de autor 2010 Saber Mohammed Abd–Allah https://creativecommons.org/licenses/by-nc/4.0
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-154
container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Español
format Artículo revista
topic bovino
semen
jalea real
longevidad
viabilidad
cattle
sperm
royal jelly
longevity
viability
spellingShingle bovino
semen
jalea real
longevidad
viabilidad
cattle
sperm
royal jelly
longevity
viability
Abd–Allah, Saber Mohammed
Effect of royal jelly on bovine sperm characteristics during post–thaw incubation in vitro
topic_facet bovino
semen
jalea real
longevidad
viabilidad
cattle
sperm
royal jelly
longevity
viability
author Abd–Allah, Saber Mohammed
author_facet Abd–Allah, Saber Mohammed
author_sort Abd–Allah, Saber Mohammed
title Effect of royal jelly on bovine sperm characteristics during post–thaw incubation in vitro
title_short Effect of royal jelly on bovine sperm characteristics during post–thaw incubation in vitro
title_full Effect of royal jelly on bovine sperm characteristics during post–thaw incubation in vitro
title_fullStr Effect of royal jelly on bovine sperm characteristics during post–thaw incubation in vitro
title_full_unstemmed Effect of royal jelly on bovine sperm characteristics during post–thaw incubation in vitro
title_sort effect of royal jelly on bovine sperm characteristics during post–thaw incubation in vitro
description Cryopreserved bovine semen is generally considered to have lower fertility compared to fresh semen. The reduction arises from both a lower post–thaw viability and a possible sublethal dysfunction of the surviving sperm population. The present study was conducted to observe the effect of royal jelly (RJ) on motility, viability and acrosome integrity of spermatozoa during post–thaw incubation. Frozen–thawed semen samples were washed and incubated at 37°C in Tris buffer containing 0.1, 0.2, 0.3, 0.4, 0.5% RJ or none (control). Sperm motility, viability and acrosomal integrity were assessed at 0, 0.5, 1, 1.5 and 2 h. The percentages of sperm motility, viability and intact acrosome were higher in Tris buffer containing 0.4% RJ compared to control (p < 0.05). After 2 h of incubation the percentages of motility, viability and intact acrosome of spermatozoa, respectively, were 52.3; 52.5 and 19.8% in 0.4% RJ containing buffer. Results indicate that the addition of 0.4% RJ in the incubation media was able to maintain better quality and longevity of spermatozoa. Royal jelly may be used as a semen extender to improve sperm quality and fertility.
publisher Universidad Nacional del Nordeste
publishDate 2010
url https://revistas.unne.edu.ar/index.php/vet/article/view/1747
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first_indexed 2025-05-17T05:08:04Z
last_indexed 2025-05-17T05:08:04Z
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