Effect of growth hormone on semen parameters, partial hemogram, and testosterone level in Nili Ravi buffalo bulls
The purpose of this trial was to examine the possible effects of exogenous growth hormone (GH) on semen quality, hematological variables and serum testosterone concentration in Nili Ravi buffalo. Bulls of the treatment group (n=3) were injected with recombinant GH 500 mg in a 15 day interval for 10...
| Autores principales: | , , , , |
|---|---|
| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Universidad Nacional del Nordeste
2016
|
| Materias: | |
| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/1086 |
| Aporte de: |
| id |
I48-R154-article-1086 |
|---|---|
| record_format |
ojs |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-154 |
| container_title_str |
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
búfalo somatotropina semen sangre testosterona. Buffalo somatotropin semen blood testosterone |
| spellingShingle |
búfalo somatotropina semen sangre testosterona. Buffalo somatotropin semen blood testosterone Masood, A. Ahmad, I. Ahmad, N. Qureshi, Z. I. Zubair, M. Effect of growth hormone on semen parameters, partial hemogram, and testosterone level in Nili Ravi buffalo bulls |
| topic_facet |
búfalo somatotropina semen sangre testosterona. Buffalo somatotropin semen blood testosterone |
| author |
Masood, A. Ahmad, I. Ahmad, N. Qureshi, Z. I. Zubair, M. |
| author_facet |
Masood, A. Ahmad, I. Ahmad, N. Qureshi, Z. I. Zubair, M. |
| author_sort |
Masood, A. |
| title |
Effect of growth hormone on semen parameters, partial hemogram, and testosterone level in Nili Ravi buffalo bulls |
| title_short |
Effect of growth hormone on semen parameters, partial hemogram, and testosterone level in Nili Ravi buffalo bulls |
| title_full |
Effect of growth hormone on semen parameters, partial hemogram, and testosterone level in Nili Ravi buffalo bulls |
| title_fullStr |
Effect of growth hormone on semen parameters, partial hemogram, and testosterone level in Nili Ravi buffalo bulls |
| title_full_unstemmed |
Effect of growth hormone on semen parameters, partial hemogram, and testosterone level in Nili Ravi buffalo bulls |
| title_sort |
effect of growth hormone on semen parameters, partial hemogram, and testosterone level in nili ravi buffalo bulls |
| description |
The purpose of this trial was to examine the possible effects of exogenous growth hormone (GH) on semen quality, hematological variables and serum testosterone concentration in Nili Ravi buffalo. Bulls of the treatment group (n=3) were injected with recombinant GH 500 mg in a 15 day interval for 10 consecutive weeks, while bulls of control group (n=3) received equal volume of normal saline solution. Semen samples were collected twice per week and analyzed for physiological parameters. Blood samples collected fortnightly were analyzed for red blood cells count (RBC), hemoglobin concentration (Hb), packed cell volume (PCV), mean corpuscular volume (MCV), white blood cell (WBC), neutrophils, lymphocytes, and serum testosterone levels. Data were analyzed statistically using t-test. Results indicated that GH treatment of Nili-Ravi buffaloes significantly (p<0.05) increased ejaculatory volume (8.8±0.2 vs 7.7±0.2 ml), sperm motility (72.6±0.4 vs 67.4±0.7%), mass activity (3.35±0.07 vs 2.52±0.08), sperm concentration (982.2±67.8 vs 731.9±50.5 million/ml), live sperm (80.1±0.3 vs 75.8±0.5%) and serum testosterone (4.02±0.21 vs 2.37±0.07 ng/ml) compared to control bulls. Among hematological variables, lymphocytes were increased (p<0.05), but MCV, WBC count, and neutrophils decreased (p<0.05). Hb, RBC and PCV remained unchanged. However, values of these variables were within normal ranges for buffalo bulls. In conclusion, treatment of Nili-Ravi bulls with GH improved semen quality and increased serum testosterone without ostensible adverse effects on the general state. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2016 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/1086 |
| work_keys_str_mv |
AT masooda effectofgrowthhormoneonsemenparameterspartialhemogramandtestosteronelevelinniliravibuffalobulls AT ahmadi effectofgrowthhormoneonsemenparameterspartialhemogramandtestosteronelevelinniliravibuffalobulls AT ahmadn effectofgrowthhormoneonsemenparameterspartialhemogramandtestosteronelevelinniliravibuffalobulls AT qureshizi effectofgrowthhormoneonsemenparameterspartialhemogramandtestosteronelevelinniliravibuffalobulls AT zubairm effectofgrowthhormoneonsemenparameterspartialhemogramandtestosteronelevelinniliravibuffalobulls AT masooda efectodelahormonadecrecimientosobrelosparametrosseminaleselhemogramaparcialyelniveldetestosteronaenbufalosniliravi AT ahmadi efectodelahormonadecrecimientosobrelosparametrosseminaleselhemogramaparcialyelniveldetestosteronaenbufalosniliravi AT ahmadn efectodelahormonadecrecimientosobrelosparametrosseminaleselhemogramaparcialyelniveldetestosteronaenbufalosniliravi AT qureshizi efectodelahormonadecrecimientosobrelosparametrosseminaleselhemogramaparcialyelniveldetestosteronaenbufalosniliravi AT zubairm efectodelahormonadecrecimientosobrelosparametrosseminaleselhemogramaparcialyelniveldetestosteronaenbufalosniliravi |
| first_indexed |
2025-05-17T05:07:49Z |
| last_indexed |
2025-05-17T05:07:49Z |
| _version_ |
1832343011712827392 |
| spelling |
I48-R154-article-10862025-02-12T21:57:19Z Effect of growth hormone on semen parameters, partial hemogram, and testosterone level in Nili Ravi buffalo bulls Efecto de la hormona de crecimiento sobre los parámetros seminales, el hemograma parcial y el nivel de testosterona en búfalos Nili Ravi Masood, A. Ahmad, I. Ahmad, N. Qureshi, Z. I. Zubair, M. búfalo somatotropina semen sangre testosterona. Buffalo somatotropin semen blood testosterone The purpose of this trial was to examine the possible effects of exogenous growth hormone (GH) on semen quality, hematological variables and serum testosterone concentration in Nili Ravi buffalo. Bulls of the treatment group (n=3) were injected with recombinant GH 500 mg in a 15 day interval for 10 consecutive weeks, while bulls of control group (n=3) received equal volume of normal saline solution. Semen samples were collected twice per week and analyzed for physiological parameters. Blood samples collected fortnightly were analyzed for red blood cells count (RBC), hemoglobin concentration (Hb), packed cell volume (PCV), mean corpuscular volume (MCV), white blood cell (WBC), neutrophils, lymphocytes, and serum testosterone levels. Data were analyzed statistically using t-test. Results indicated that GH treatment of Nili-Ravi buffaloes significantly (p<0.05) increased ejaculatory volume (8.8±0.2 vs 7.7±0.2 ml), sperm motility (72.6±0.4 vs 67.4±0.7%), mass activity (3.35±0.07 vs 2.52±0.08), sperm concentration (982.2±67.8 vs 731.9±50.5 million/ml), live sperm (80.1±0.3 vs 75.8±0.5%) and serum testosterone (4.02±0.21 vs 2.37±0.07 ng/ml) compared to control bulls. Among hematological variables, lymphocytes were increased (p<0.05), but MCV, WBC count, and neutrophils decreased (p<0.05). Hb, RBC and PCV remained unchanged. However, values of these variables were within normal ranges for buffalo bulls. In conclusion, treatment of Nili-Ravi bulls with GH improved semen quality and increased serum testosterone without ostensible adverse effects on the general state. El propósito de este ensayo fue examinar los posibles efectos de la hormona de crecimiento exógena (GH) sobre la calidad del semen, algunas variables del hemograma y la concentración de testosterona en suero de toros bubalinos Nili Ravi. Los búfalos del grupo tratado (n=3) fueron inyectados con 500 mg de somatotropina recombinante cada 15 días durante 10 semanas consecutivas, mientras que los del grupo control (n=3) recibieron igual volumen de solución fisiológica salina. Las muestras de semen fueron tomadas dos veces por semana y analizadas para evaluar los parámetros fisiológicos. Las muestras de sangre colectadas quincenalmente fueron analizadas para establecer el recuento de glóbulos rojos, la concentración de hemoglobina, el hematocrito, el volumen corpuscular medio (MCV), recuento de glóbulos blancos (WBC), tasa de neutrófilos y linfocitos, así como los niveles séricos de testosterona. Los datos obtenidos fueron analizados estadísticamente por medio del test-t de Student. Los resultados indicaron que el tratamiento con somatotropina aumentó significativamente (p<0,05) el volumen eyaculado (8,8±0,2 versus 7,7±0,2 ml), la motilidad del semen (72,6±0,4 versus 67,4±0,7%), la actividad de masa (3,35±0,07 versus 2,52±0,08), la concentración de espermatozoides (982,2±67,8 versus 731,9±50,5 millones/ml), los espermatozoides vivos (80,1±0,3 versus 75,8±0,5%) y el nivel de testosterona sérica (4,02±0,21 versus 2,37±0,07 ng/ml), comparados con los animales controles. En el hemograma aumentaron los linfocitos (p<0,05) y disminuyeron variables como volumen corpuscular medio, recuento de leucocitos y tasa de neutrófilos (p<0,05). Hemoglobina, concentración de eritrocitos y hematocrito permanecieron inalterados. Todos los parámetros investigados se situaron dentro de los rangos normales para toros bubalinos. En conclusión, el tratamiento de búfalos Nili-Ravi con somatotropina mejoró la calidad del semen y aumentó la testosterona sérica, sin provocar ostensibles efectos adversos en el estado general de los toros Universidad Nacional del Nordeste 2016-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/1086 10.30972/vet.2721086 Revista Veterinaria; Vol. 27 Núm. 2 (2016); 98-102 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/1086/887 Derechos de autor 2016 A. Masood, I. Ahmad, N. Ahmad, Z. I. Qureshi, M. Zubair https://creativecommons.org/licenses/by-nc/4.0 |