Peptidase from Aspergillus niger NRRL 3 : optimization of its production by solid-state fermentation, purification and characterization

Abstract: Orange peels, soybean hulls, Ilex paraguariensis and Platanus x hispanica were evaluated as solid substrates in order to produce peptidases from Aspergillus niger NRRL3 (PAN) under solid-state fermentation. The mixture of soybean hulls and orange peels enabled fungal development and showed...

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Autores principales: López, Débora Natalia, Galante, Micaela, Ruggieri, Germán, Piaruchi, Julia, Dib, María E., Montellano Duran, Natalia, Lombardi, Julia, De Sanctis, Mariana, Boeris, Valeria, Risso, Patricia H., Spelzini, Darío
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2019
Materias:
ASPERGILLUS NIGER
ENZIMAS
MANEJO DE DESECHOS
DESECHOS AGRICOLAS
DESECHOS INDUSTRIALES
FERMENTACION
RESIDUOS SOLIDOS
PURIFICACION
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/8366
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-8366
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic ASPERGILLUS NIGER
ENZIMAS
MANEJO DE DESECHOS
DESECHOS AGRICOLAS
DESECHOS INDUSTRIALES
FERMENTACION
RESIDUOS SOLIDOS
PURIFICACION
spellingShingle ASPERGILLUS NIGER
ENZIMAS
MANEJO DE DESECHOS
DESECHOS AGRICOLAS
DESECHOS INDUSTRIALES
FERMENTACION
RESIDUOS SOLIDOS
PURIFICACION
López, Débora Natalia
Galante, Micaela
Ruggieri, Germán
Piaruchi, Julia
Dib, María E.
Montellano Duran, Natalia
Lombardi, Julia
De Sanctis, Mariana
Boeris, Valeria
Risso, Patricia H.
Spelzini, Darío
Peptidase from Aspergillus niger NRRL 3 : optimization of its production by solid-state fermentation, purification and characterization
topic_facet ASPERGILLUS NIGER
ENZIMAS
MANEJO DE DESECHOS
DESECHOS AGRICOLAS
DESECHOS INDUSTRIALES
FERMENTACION
RESIDUOS SOLIDOS
PURIFICACION
description Abstract: Orange peels, soybean hulls, Ilex paraguariensis and Platanus x hispanica were evaluated as solid substrates in order to produce peptidases from Aspergillus niger NRRL3 (PAN) under solid-state fermentation. The mixture of soybean hulls and orange peels enabled fungal development and showed the highest peptidase production. The optimal conditions for PAN production were found to be as follows: soybean hulls/orange peels mass ratio, 0.25; initial pH, 7.05; K2HPO4 43.5 g/L and 4.03 g/L NaNO3; inoculation with 5000 conidia per 3 g of solid substrate; incubation conditions, 30 °C for 5 days. Under these conditions, the peptidase activity was 1000 ± 100 AU/mL. PAN concentration was performed by adsorption on a DEAE-cellulose matrix. The subsequent purification was carried out by gel filtration on Sephadex G-100, with a global purification factor of about 9. PAN proved to belong to the serine-type of peptidases, its highest peptidase activity being at 65 °C. However, proteolysis at 60 °C proved more suitable due to the differences in the inactivation rate. Besides, PAN showed high stability over a pH range of 4–11. Taking all this into account, we herein describe the production and purification of a serine peptidase from Aspergillus niger NRRL3 for the first time.
format Artículo
author López, Débora Natalia
Galante, Micaela
Ruggieri, Germán
Piaruchi, Julia
Dib, María E.
Montellano Duran, Natalia
Lombardi, Julia
De Sanctis, Mariana
Boeris, Valeria
Risso, Patricia H.
Spelzini, Darío
author_facet López, Débora Natalia
Galante, Micaela
Ruggieri, Germán
Piaruchi, Julia
Dib, María E.
Montellano Duran, Natalia
Lombardi, Julia
De Sanctis, Mariana
Boeris, Valeria
Risso, Patricia H.
Spelzini, Darío
author_sort López, Débora Natalia
title Peptidase from Aspergillus niger NRRL 3 : optimization of its production by solid-state fermentation, purification and characterization
title_short Peptidase from Aspergillus niger NRRL 3 : optimization of its production by solid-state fermentation, purification and characterization
title_full Peptidase from Aspergillus niger NRRL 3 : optimization of its production by solid-state fermentation, purification and characterization
title_fullStr Peptidase from Aspergillus niger NRRL 3 : optimization of its production by solid-state fermentation, purification and characterization
title_full_unstemmed Peptidase from Aspergillus niger NRRL 3 : optimization of its production by solid-state fermentation, purification and characterization
title_sort peptidase from aspergillus niger nrrl 3 : optimization of its production by solid-state fermentation, purification and characterization
publisher Elsevier
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/8366
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