Evaluation of viral concentration methods for SARS-CoV-2 recovery from wastewaters
Wastewater-based epidemiology (WBE) is a useful tool that has the potential to act as a complementary ap-proach to monitor the presence of SARS-CoV-2 in the community and as an early alarm system for COVID-19 out-break. Many studies reported low concentrations of SARS-CoV-2 in sewage and also reveal...
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| Autores principales: | , , , , , , , |
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| Formato: | Articulo article acceptedVersion |
| Lenguaje: | Inglés |
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Elsevier
2021
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| Acceso en línea: | http://rdi.uncoma.edu.ar/handle/uncomaid/16527 https://www.sciencedirect.com/science/article/pii/S0048969720376361 |
| Aporte de: |
| id |
I22-R178-uncomaid-16527 |
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| record_format |
dspace |
| institution |
Universidad Nacional del Comahue |
| institution_str |
I-22 |
| repository_str |
R-178 |
| collection |
Repositorio Institucional UNCo |
| language |
Inglés |
| topic |
COVID-19 surveillance Sewage Wastewater-based epidemiology Viral concentration methods COVID-19 Ciencias Sociales Ciencias Aplicadas |
| spellingShingle |
COVID-19 surveillance Sewage Wastewater-based epidemiology Viral concentration methods COVID-19 Ciencias Sociales Ciencias Aplicadas Barril, Patricia Angelica Pianciola, Luis Alfredo Mazzeo, Melina Ousset, Maria Julia Jaureguiberry, María Virginia Alessandrello, Mauricio Sanchez, Gloria Oteiza, Juan Martín Evaluation of viral concentration methods for SARS-CoV-2 recovery from wastewaters |
| topic_facet |
COVID-19 surveillance Sewage Wastewater-based epidemiology Viral concentration methods COVID-19 Ciencias Sociales Ciencias Aplicadas |
| description |
Wastewater-based epidemiology (WBE) is a useful tool that has the potential to act as a complementary ap-proach to monitor the presence of SARS-CoV-2 in the community and as an early alarm system for COVID-19 out-break. Many studies reported low concentrations of SARS-CoV-2 in sewage and also revealed the need formethodological validation for enveloped viruses concentration in wastewater. The aim of this study was to eval-uate different methodologies for the concentration of viruses in wastewaters and to select and improve an optionthat maximizes the recovery of SARS-CoV-2. A total of 11 concentration techniques based on different principleswere evaluated: adsorption-elution protocolswith negatively chargedmembranesfollowedbypolyethylenegly-col (PEG) precipitation (Methods 1–2), PEG precipitation (Methods 3–7), aluminum polychloride (PAC)floccu-lation (Method 8), ultrafiltration (Method 9), skim milkflocculation (Method 10) and adsorption-elution withnegatively charged membrane followed by ultrafiltration (Method 11). To evaluate the performance of theseconcentration techniques, feline calicivirus (FCV) was used as a process control in order to avoid the risk associ-ated with handling SARS-CoV-2. Two protocols, one based on PEG precipitation and the other on PACfloccula-tion, showed high efficiency for FCV recovery from wastewater (62.2% and 45.0%, respectively). These twomethods were then tested for the specific recovery of SARS-CoV-2. Both techniques could recover SARS-CoV-2from wastewater, PACflocculation showed a lower limit of detection (4.3 × 102GC/mL) than PEG precipitation (4.3 × 103GC/mL). This work provides a critical overview of current methods used for virus concentration inwastewaters and the analysis of sensitivity for the specific recovery of SARS-CoV-2 in sewage. The data obtainedhere highlights the viability of WBE for the surveillance of COVID-19 infections in the community. |
| format |
Articulo article acceptedVersion |
| author |
Barril, Patricia Angelica Pianciola, Luis Alfredo Mazzeo, Melina Ousset, Maria Julia Jaureguiberry, María Virginia Alessandrello, Mauricio Sanchez, Gloria Oteiza, Juan Martín |
| author_facet |
Barril, Patricia Angelica Pianciola, Luis Alfredo Mazzeo, Melina Ousset, Maria Julia Jaureguiberry, María Virginia Alessandrello, Mauricio Sanchez, Gloria Oteiza, Juan Martín |
| author_sort |
Barril, Patricia Angelica |
| title |
Evaluation of viral concentration methods for SARS-CoV-2 recovery from wastewaters |
| title_short |
Evaluation of viral concentration methods for SARS-CoV-2 recovery from wastewaters |
| title_full |
Evaluation of viral concentration methods for SARS-CoV-2 recovery from wastewaters |
| title_fullStr |
Evaluation of viral concentration methods for SARS-CoV-2 recovery from wastewaters |
| title_full_unstemmed |
Evaluation of viral concentration methods for SARS-CoV-2 recovery from wastewaters |
| title_sort |
evaluation of viral concentration methods for sars-cov-2 recovery from wastewaters |
| publisher |
Elsevier |
| publishDate |
2021 |
| url |
http://rdi.uncoma.edu.ar/handle/uncomaid/16527 https://www.sciencedirect.com/science/article/pii/S0048969720376361 |
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