Evaluation of enzymatic Pectin extraction by a recombinant Polygalacturonase (PGI) from apples and pears pomace of argentinean production and characterization of the extracted pectin

Argentina produces, annually, 1.8 million tons of pome fruit, distributed almost equally between apple and pear. These fruits, produced in the High Valley of Río Negro are already a registered brand in the world of fruit. Aspergillus kawachii produces an acidic polygalacturonase (PGase) called PGI t...

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Detalles Bibliográficos
Autores principales: Franchi, María Luisa, Marzialetti, María Belén, Pose, Graciela Noemí, Cavalitto, Sebastián Fernando
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Química
Polygalacturonase
Pectin extraction
Apples
Pears
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/98995
https://ri.conicet.gov.ar/11336/16112
https://www.longdom.org/abstract/evaluation-of-enzymatic-pectin-extraction-by-a-recombinant-polygalacturonase-pgi-from-apples-and-pears-pomace-of-argenti-33919.html
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-98995
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Polygalacturonase
Pectin extraction
Apples
Pears
spellingShingle Química
Polygalacturonase
Pectin extraction
Apples
Pears
Franchi, María Luisa
Marzialetti, María Belén
Pose, Graciela Noemí
Cavalitto, Sebastián Fernando
Evaluation of enzymatic Pectin extraction by a recombinant Polygalacturonase (PGI) from apples and pears pomace of argentinean production and characterization of the extracted pectin
topic_facet Química
Polygalacturonase
Pectin extraction
Apples
Pears
description Argentina produces, annually, 1.8 million tons of pome fruit, distributed almost equally between apple and pear. These fruits, produced in the High Valley of Río Negro are already a registered brand in the world of fruit. Aspergillus kawachii produces an acidic polygalacturonase (PGase) called PGI that has attracted considerable interest because of its potential use in the food industry, particularly those involving fruit production in this region (such as pectin extraction). The enzyme was cloned and expressed in Saccharomyces cerevisiae. The objective of this work was to evaluate the use of PG1 in the enzymatic pectin extraction from apples and pears pomace. Characterization of the extracted pectin was also done. The performance of PGI extraction process was compared to the traditional chemical extraction process and to the enzymatic extraction with commercial enzymes. The esterification degree and the content of uronic acids from the pectin obtained were determined. In all cases, the extraction with PGI had higher yields than the chemical extraction process. Enzymatic extracted pectins exhibited an esterification degree> 50% so that they can be considered belonging to the group of high methoxylpectins. According to these results, PGI could be used to produce pectins from fruit pomaces converting these materials, currently considered wastes, into by-products of the fruit industry.
format Articulo
Articulo
author Franchi, María Luisa
Marzialetti, María Belén
Pose, Graciela Noemí
Cavalitto, Sebastián Fernando
author_facet Franchi, María Luisa
Marzialetti, María Belén
Pose, Graciela Noemí
Cavalitto, Sebastián Fernando
author_sort Franchi, María Luisa
title Evaluation of enzymatic Pectin extraction by a recombinant Polygalacturonase (PGI) from apples and pears pomace of argentinean production and characterization of the extracted pectin
title_short Evaluation of enzymatic Pectin extraction by a recombinant Polygalacturonase (PGI) from apples and pears pomace of argentinean production and characterization of the extracted pectin
title_full Evaluation of enzymatic Pectin extraction by a recombinant Polygalacturonase (PGI) from apples and pears pomace of argentinean production and characterization of the extracted pectin
title_fullStr Evaluation of enzymatic Pectin extraction by a recombinant Polygalacturonase (PGI) from apples and pears pomace of argentinean production and characterization of the extracted pectin
title_full_unstemmed Evaluation of enzymatic Pectin extraction by a recombinant Polygalacturonase (PGI) from apples and pears pomace of argentinean production and characterization of the extracted pectin
title_sort evaluation of enzymatic pectin extraction by a recombinant polygalacturonase (pgi) from apples and pears pomace of argentinean production and characterization of the extracted pectin
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/98995
https://ri.conicet.gov.ar/11336/16112
https://www.longdom.org/abstract/evaluation-of-enzymatic-pectin-extraction-by-a-recombinant-polygalacturonase-pgi-from-apples-and-pears-pomace-of-argenti-33919.html
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