Shiga toxin-producing <i>Escherichia coli</i> in beef retail markets from Argentina

Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens that cause mild or serious diseases and can lead to people death. This study reports the prevalence and characteristics of STEC O157 and non-O157 in commercial ground beef and environmental samples, including meat table, knife, me...

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Autores principales: Brusa, Victoria, Aliverti, Virginia, Aliverti, Florencia, Ortega, Emanuel, Torre, Julián Horacio de la, Linares, Luciano Héctor, Sanz, Marcelo E., Etcheverría, Analía I., Padola, Nora Lía, Galli, Lucía, Peral García, Pilar, Copes, Julio Alberto, Leotta, Gerardo Aníbal
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Ciencias Veterinarias
Argentina
Environmental samples
Raw ground beef
Retail markets
Stec
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85414
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85414
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Argentina
Environmental samples
Raw ground beef
Retail markets
Stec
spellingShingle Ciencias Veterinarias
Argentina
Environmental samples
Raw ground beef
Retail markets
Stec
Brusa, Victoria
Aliverti, Virginia
Aliverti, Florencia
Ortega, Emanuel
Torre, Julián Horacio de la
Linares, Luciano Héctor
Sanz, Marcelo E.
Etcheverría, Analía I.
Padola, Nora Lía
Galli, Lucía
Peral García, Pilar
Copes, Julio Alberto
Leotta, Gerardo Aníbal
Shiga toxin-producing <i>Escherichia coli</i> in beef retail markets from Argentina
topic_facet Ciencias Veterinarias
Argentina
Environmental samples
Raw ground beef
Retail markets
Stec
description Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens that cause mild or serious diseases and can lead to people death. This study reports the prevalence and characteristics of STEC O157 and non-O157 in commercial ground beef and environmental samples, including meat table, knife, meat mincing machine, and manipulator hands (n = 450) obtained from 90 retail markets over a nine-month period. The STEC isolates were serotyped and virulence genes as stx (Shiga toxin), rfbO157] (O157 lipopolysaccharide), fliCH7 (H7 flagellin), eae (intimin), ehxA (enterohemolysin) and saa (STEC autoagglutinating adhesin), were determined. STEC O157 were identified in 23 (25.5%) beef samples and 16 (4.4%) environmental samples, while STEC non-O157 were present in 47 (52.2%) and 182 (50.5%), respectively. Among 54 strains isolated, 17 were STEC O157:H7 and 37 were STEC non-O157. The prevalent genotype for O157 was stx2/eae/ehxA/fliCH7 (83.4%), and for STEC non-O157 the most frequent ones were stx1/stx2/saa/ehxA (29.7%); stx2 (29.7%); and stx2/saa/ehxA (27%). None of the STEC non-O157 strains were eae-positive. Besides O157:H7, other 20 different serotypes were identified, being O8:H19, O178:H19, and O174:H28 the prevalent. Strains belonging to the same serotype could be isolated from different sources of the same retail market. Also, the same serotype could be detected in different stores. In conclusion, screening techniques are increasingly sensitive, but the isolation of STEC non-O157 is still a challenge. Moreover, with the results obtained from the present work, although more studies are needed, cross-contamination between meat and the environment could be suspected.
format Articulo
Articulo
author Brusa, Victoria
Aliverti, Virginia
Aliverti, Florencia
Ortega, Emanuel
Torre, Julián Horacio de la
Linares, Luciano Héctor
Sanz, Marcelo E.
Etcheverría, Analía I.
Padola, Nora Lía
Galli, Lucía
Peral García, Pilar
Copes, Julio Alberto
Leotta, Gerardo Aníbal
author_facet Brusa, Victoria
Aliverti, Virginia
Aliverti, Florencia
Ortega, Emanuel
Torre, Julián Horacio de la
Linares, Luciano Héctor
Sanz, Marcelo E.
Etcheverría, Analía I.
Padola, Nora Lía
Galli, Lucía
Peral García, Pilar
Copes, Julio Alberto
Leotta, Gerardo Aníbal
author_sort Brusa, Victoria
title Shiga toxin-producing <i>Escherichia coli</i> in beef retail markets from Argentina
title_short Shiga toxin-producing <i>Escherichia coli</i> in beef retail markets from Argentina
title_full Shiga toxin-producing <i>Escherichia coli</i> in beef retail markets from Argentina
title_fullStr Shiga toxin-producing <i>Escherichia coli</i> in beef retail markets from Argentina
title_full_unstemmed Shiga toxin-producing <i>Escherichia coli</i> in beef retail markets from Argentina
title_sort shiga toxin-producing <i>escherichia coli</i> in beef retail markets from argentina
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/85414
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