Triptolide modulates adriamycin sensitivity via regulating Mir-21 and Bcl-2 expression in K562/A02 cell line
Drug resistance is a major obstacle for successful treatment of leukemia. Increasing evidence suggests that microRNA-21 (miR-21) is over-expressed in K562/A02 cell line, promoting drug resistance. The aim of our present study is to investigate the reversal effects of triptolide on drug resistance in...
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Formato: | Articulo |
Lenguaje: | Inglés |
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2011
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/8395 http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_1_7.pdf |
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I19-R120-10915-8395 |
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institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
collection |
SEDICI (UNLP) |
language |
Inglés |
topic |
Farmacia Leucemia Resistencia a Medicamentos MicroARNs |
spellingShingle |
Farmacia Leucemia Resistencia a Medicamentos MicroARNs Zhu, Xiaolan Long, Lulu Chen, Qiaoyun Shen, Huiling Xu, Wenlin Hui, Lulu Li, Hao Triptolide modulates adriamycin sensitivity via regulating Mir-21 and Bcl-2 expression in K562/A02 cell line |
topic_facet |
Farmacia Leucemia Resistencia a Medicamentos MicroARNs |
description |
Drug resistance is a major obstacle for successful treatment of leukemia. Increasing evidence suggests that microRNA-21 (miR-21) is over-expressed in K562/A02 cell line, promoting drug resistance. The aim of our present study is to investigate the reversal effects of triptolide on drug resistance in adriamycin-resistant cells. Cell viability was measured by MTT assays and adriamycin induced apoptosis was evaluated by flow cytometry. Levels of miR-21 quantified by real-time PCR. Bcl-2 protein level were measured by western blot. TPL enhanced sensitivity of K562/A02 cells to adriamycin and promoted adriamycin–induced apoptosis. Levels of miR-21 and Bcl-2 was significantly decreased after triptolide treatment. Transfection with anti-miR-21, a significant up-regulation of sensitivity to adriamycin and a significant down-regulation of Bcl-2 protein level was noted in K562/A02 cells. Our study suggests that triptolide significantly sensitizes K562/A02 cell to adriamycin by inducing apoptosis and these effects of triptolide may be due to its down-regulation of miR-21. |
format |
Articulo Articulo |
author |
Zhu, Xiaolan Long, Lulu Chen, Qiaoyun Shen, Huiling Xu, Wenlin Hui, Lulu Li, Hao |
author_facet |
Zhu, Xiaolan Long, Lulu Chen, Qiaoyun Shen, Huiling Xu, Wenlin Hui, Lulu Li, Hao |
author_sort |
Zhu, Xiaolan |
title |
Triptolide modulates adriamycin sensitivity via regulating Mir-21 and Bcl-2 expression in K562/A02 cell line |
title_short |
Triptolide modulates adriamycin sensitivity via regulating Mir-21 and Bcl-2 expression in K562/A02 cell line |
title_full |
Triptolide modulates adriamycin sensitivity via regulating Mir-21 and Bcl-2 expression in K562/A02 cell line |
title_fullStr |
Triptolide modulates adriamycin sensitivity via regulating Mir-21 and Bcl-2 expression in K562/A02 cell line |
title_full_unstemmed |
Triptolide modulates adriamycin sensitivity via regulating Mir-21 and Bcl-2 expression in K562/A02 cell line |
title_sort |
triptolide modulates adriamycin sensitivity via regulating mir-21 and bcl-2 expression in k562/a02 cell line |
publishDate |
2011 |
url |
http://sedici.unlp.edu.ar/handle/10915/8395 http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_1_7.pdf |
work_keys_str_mv |
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bdutipo_str |
Repositorios |
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1764820488216903685 |