Triptolide modulates adriamycin sensitivity via regulating Mir-21 and Bcl-2 expression in K562/A02 cell line

Drug resistance is a major obstacle for successful treatment of leukemia. Increasing evidence suggests that microRNA-21 (miR-21) is over-expressed in K562/A02 cell line, promoting drug resistance. The aim of our present study is to investigate the reversal effects of triptolide on drug resistance in...

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Autores principales: Zhu, Xiaolan, Long, Lulu, Chen, Qiaoyun, Shen, Huiling, Xu, Wenlin, Hui, Lulu, Li, Hao
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
Leucemia
Resistencia a Medicamentos
MicroARNs
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8395
http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_1_7.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8395
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Leucemia
Resistencia a Medicamentos
MicroARNs
spellingShingle Farmacia
Leucemia
Resistencia a Medicamentos
MicroARNs
Zhu, Xiaolan
Long, Lulu
Chen, Qiaoyun
Shen, Huiling
Xu, Wenlin
Hui, Lulu
Li, Hao
Triptolide modulates adriamycin sensitivity via regulating Mir-21 and Bcl-2 expression in K562/A02 cell line
topic_facet Farmacia
Leucemia
Resistencia a Medicamentos
MicroARNs
description Drug resistance is a major obstacle for successful treatment of leukemia. Increasing evidence suggests that microRNA-21 (miR-21) is over-expressed in K562/A02 cell line, promoting drug resistance. The aim of our present study is to investigate the reversal effects of triptolide on drug resistance in adriamycin-resistant cells. Cell viability was measured by MTT assays and adriamycin induced apoptosis was evaluated by flow cytometry. Levels of miR-21 quantified by real-time PCR. Bcl-2 protein level were measured by western blot. TPL enhanced sensitivity of K562/A02 cells to adriamycin and promoted adriamycin–induced apoptosis. Levels of miR-21 and Bcl-2 was significantly decreased after triptolide treatment. Transfection with anti-miR-21, a significant up-regulation of sensitivity to adriamycin and a significant down-regulation of Bcl-2 protein level was noted in K562/A02 cells. Our study suggests that triptolide significantly sensitizes K562/A02 cell to adriamycin by inducing apoptosis and these effects of triptolide may be due to its down-regulation of miR-21.
format Articulo
Articulo
author Zhu, Xiaolan
Long, Lulu
Chen, Qiaoyun
Shen, Huiling
Xu, Wenlin
Hui, Lulu
Li, Hao
author_facet Zhu, Xiaolan
Long, Lulu
Chen, Qiaoyun
Shen, Huiling
Xu, Wenlin
Hui, Lulu
Li, Hao
author_sort Zhu, Xiaolan
title Triptolide modulates adriamycin sensitivity via regulating Mir-21 and Bcl-2 expression in K562/A02 cell line
title_short Triptolide modulates adriamycin sensitivity via regulating Mir-21 and Bcl-2 expression in K562/A02 cell line
title_full Triptolide modulates adriamycin sensitivity via regulating Mir-21 and Bcl-2 expression in K562/A02 cell line
title_fullStr Triptolide modulates adriamycin sensitivity via regulating Mir-21 and Bcl-2 expression in K562/A02 cell line
title_full_unstemmed Triptolide modulates adriamycin sensitivity via regulating Mir-21 and Bcl-2 expression in K562/A02 cell line
title_sort triptolide modulates adriamycin sensitivity via regulating mir-21 and bcl-2 expression in k562/a02 cell line
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8395
http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_1_7.pdf
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