Exopolysaccharides produced by Lactobacillus and Bifidobacterium strains abrogate in vitro the cytotoxic effect of bacterial toxins on eukaryotic cells

Aims: To evaluate the capability of the exopolysaccharides (EPS) produced by lactobacilli and bifidobacteria from human and dairy origin to antagonize the cytotoxic effect of bacterial toxins. Methods and Results: The cytotoxicity of <i>Bacillus cereus</i> extracellular factors on Caco-...

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Detalles Bibliográficos
Autores principales: Ruas Madiedo, P., Medrano, Micaela, Salazar, N., Reyes Gavilán, C. G. de los, Pérez, Pablo Fernando, Abraham, Analía Graciela
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Ciencias Exactas
Bacillus cereus
Bifidobacterium
Caco-2
Erythrocytes
Exopolysaccharides
Lactobacillus
streptolysin-O
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82612
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82612
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Bacillus cereus
Bifidobacterium
Caco-2
Erythrocytes
Exopolysaccharides
Lactobacillus
streptolysin-O
spellingShingle Ciencias Exactas
Bacillus cereus
Bifidobacterium
Caco-2
Erythrocytes
Exopolysaccharides
Lactobacillus
streptolysin-O
Ruas Madiedo, P.
Medrano, Micaela
Salazar, N.
Reyes Gavilán, C. G. de los
Pérez, Pablo Fernando
Abraham, Analía Graciela
Exopolysaccharides produced by Lactobacillus and Bifidobacterium strains abrogate in vitro the cytotoxic effect of bacterial toxins on eukaryotic cells
topic_facet Ciencias Exactas
Bacillus cereus
Bifidobacterium
Caco-2
Erythrocytes
Exopolysaccharides
Lactobacillus
streptolysin-O
description Aims: To evaluate the capability of the exopolysaccharides (EPS) produced by lactobacilli and bifidobacteria from human and dairy origin to antagonize the cytotoxic effect of bacterial toxins. Methods and Results: The cytotoxicity of <i>Bacillus cereus</i> extracellular factors on Caco-2 colonocytes in the presence/absence of the EPS was determined by measuring the integrity of the tissue monolayer and the damage to the cell membrane (extracellular lactate dehydrogenase activity). Additionally, the protective effect of EPS against the haemolytic activity of the streptolysin-O was evaluated on rabbit erythrocytes. The EPS produced by <i>Bifidobacterium animalis</i> ssp. <i>lactis</i> A1 and IPLA-R1, <i>Bifidobacterium longum</i> NB667 and <i>Lactobacillus rhamnosus</i> GG were able to counteract the toxic effect of bacterial toxins on the eukaryotic cells at 1 mg ml<SUP>-1</SUP> EPS concentration. The EPS A1 was the most effective in counteracting the effect of <i>B. cereus</i> toxins on colonocytes, even at lower doses (0.5 mg ml<SUP>-1</SUP>), whereas EPS NB667 elicited the highest haemolysis reduction on erythrocytes. Conclusions: The production of EPS by lactobacilli and bifidobacteria could antagonize the toxicity of bacterial pathogens, this effect being EPS and biological marker dependent. Significance and Impact of the Study: This work allows gaining insight about the mechanisms that probiotics could exert to improve the host health.
format Articulo
Articulo
author Ruas Madiedo, P.
Medrano, Micaela
Salazar, N.
Reyes Gavilán, C. G. de los
Pérez, Pablo Fernando
Abraham, Analía Graciela
author_facet Ruas Madiedo, P.
Medrano, Micaela
Salazar, N.
Reyes Gavilán, C. G. de los
Pérez, Pablo Fernando
Abraham, Analía Graciela
author_sort Ruas Madiedo, P.
title Exopolysaccharides produced by Lactobacillus and Bifidobacterium strains abrogate in vitro the cytotoxic effect of bacterial toxins on eukaryotic cells
title_short Exopolysaccharides produced by Lactobacillus and Bifidobacterium strains abrogate in vitro the cytotoxic effect of bacterial toxins on eukaryotic cells
title_full Exopolysaccharides produced by Lactobacillus and Bifidobacterium strains abrogate in vitro the cytotoxic effect of bacterial toxins on eukaryotic cells
title_fullStr Exopolysaccharides produced by Lactobacillus and Bifidobacterium strains abrogate in vitro the cytotoxic effect of bacterial toxins on eukaryotic cells
title_full_unstemmed Exopolysaccharides produced by Lactobacillus and Bifidobacterium strains abrogate in vitro the cytotoxic effect of bacterial toxins on eukaryotic cells
title_sort exopolysaccharides produced by lactobacillus and bifidobacterium strains abrogate in vitro the cytotoxic effect of bacterial toxins on eukaryotic cells
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/82612
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