Amaranth proteins emulsions as delivery system of Angiotensin-I converting enzyme inhibitory peptides

We analyze the possibility of using the emulsifying properties of amaranth proteins and the bioactivity shown by peptide sequences encrypted in these proteins to formulate functional emulsions with angiotensin I-converting enzyme (ACE) inhibitory activity. For this we formulate O:W emulsions, 20:80,...

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Autores principales: Suárez, Santiago Emmanuel, Añón, María Cristina
Formato: Articulo
Lenguaje:Inglés
Publicado: 2019
Materias:
Ciencias Exactas
Química
Amaranth proteins
O:W emulsions
Antihypertensive deliver
Functional foods
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/152335
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-152335
record_format dspace
spelling I19-R120-10915-1523352023-05-02T20:08:05Z http://sedici.unlp.edu.ar/handle/10915/152335 issn:0268-005X Amaranth proteins emulsions as delivery system of Angiotensin-I converting enzyme inhibitory peptides Suárez, Santiago Emmanuel Añón, María Cristina 2019-05 2023-05-02T14:05:59Z en Ciencias Exactas Química Amaranth proteins O:W emulsions Antihypertensive deliver Functional foods We analyze the possibility of using the emulsifying properties of amaranth proteins and the bioactivity shown by peptide sequences encrypted in these proteins to formulate functional emulsions with angiotensin I-converting enzyme (ACE) inhibitory activity. For this we formulate O:W emulsions, 20:80, from a mixture in equal parts (50:50) of amaranth protein isolates (API), and hydrolysates (AH) at 1 and 2% protein w/v. Results obtained showed that these emulsions, API50-AH50-1% and API50-AH50-2%, are highly flocculated (Flocculation index: 8.2 and 5.9) and stable at least for 8 days, without evident creaming or coalescence. The components present in the emulsions were capable of inhibiting ACE, in in vitro assays (IC₅₀ of 0.14 ± 0.02 mg/mL). API and API50-AH50 emulsions were subjected to a simulated gastrointestinal digestion in vitro. The emulsions were susceptible to aggregation and coalescence phenomena during this process, which could be a consequence of the chaotropic action of the bile salt on the interface and the proteolytic and lipolytic action of pancreatin and lipase, respectively (D₄.₃ of original emulsion: 1.22 ± 0.01 μm and D₄.₃ of digested emulsion 79.5 ± 17.1 μm). We also found that amaranth proteins were more resistant to gastric than duodenal digestion. After the process of simulated gastrointestinal digestion, the inhibition of ACE (IC₅₀ of 0.13 ± 0.07 mg/mL) was maintained. This fact evidences the protective effect of the emulsion on the bioavailability of the ACE inhibitory peptides, by either their participation in the formation of the interfacial film and/or their participation in the network of formed flocs. Centro de Investigación y Desarrollo en Criotecnología de Alimentos Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf 154-161
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Química
Amaranth proteins
O:W emulsions
Antihypertensive deliver
Functional foods
spellingShingle Ciencias Exactas
Química
Amaranth proteins
O:W emulsions
Antihypertensive deliver
Functional foods
Suárez, Santiago Emmanuel
Añón, María Cristina
Amaranth proteins emulsions as delivery system of Angiotensin-I converting enzyme inhibitory peptides
topic_facet Ciencias Exactas
Química
Amaranth proteins
O:W emulsions
Antihypertensive deliver
Functional foods
description We analyze the possibility of using the emulsifying properties of amaranth proteins and the bioactivity shown by peptide sequences encrypted in these proteins to formulate functional emulsions with angiotensin I-converting enzyme (ACE) inhibitory activity. For this we formulate O:W emulsions, 20:80, from a mixture in equal parts (50:50) of amaranth protein isolates (API), and hydrolysates (AH) at 1 and 2% protein w/v. Results obtained showed that these emulsions, API50-AH50-1% and API50-AH50-2%, are highly flocculated (Flocculation index: 8.2 and 5.9) and stable at least for 8 days, without evident creaming or coalescence. The components present in the emulsions were capable of inhibiting ACE, in in vitro assays (IC₅₀ of 0.14 ± 0.02 mg/mL). API and API50-AH50 emulsions were subjected to a simulated gastrointestinal digestion in vitro. The emulsions were susceptible to aggregation and coalescence phenomena during this process, which could be a consequence of the chaotropic action of the bile salt on the interface and the proteolytic and lipolytic action of pancreatin and lipase, respectively (D₄.₃ of original emulsion: 1.22 ± 0.01 μm and D₄.₃ of digested emulsion 79.5 ± 17.1 μm). We also found that amaranth proteins were more resistant to gastric than duodenal digestion. After the process of simulated gastrointestinal digestion, the inhibition of ACE (IC₅₀ of 0.13 ± 0.07 mg/mL) was maintained. This fact evidences the protective effect of the emulsion on the bioavailability of the ACE inhibitory peptides, by either their participation in the formation of the interfacial film and/or their participation in the network of formed flocs.
format Articulo
Articulo
author Suárez, Santiago Emmanuel
Añón, María Cristina
author_facet Suárez, Santiago Emmanuel
Añón, María Cristina
author_sort Suárez, Santiago Emmanuel
title Amaranth proteins emulsions as delivery system of Angiotensin-I converting enzyme inhibitory peptides
title_short Amaranth proteins emulsions as delivery system of Angiotensin-I converting enzyme inhibitory peptides
title_full Amaranth proteins emulsions as delivery system of Angiotensin-I converting enzyme inhibitory peptides
title_fullStr Amaranth proteins emulsions as delivery system of Angiotensin-I converting enzyme inhibitory peptides
title_full_unstemmed Amaranth proteins emulsions as delivery system of Angiotensin-I converting enzyme inhibitory peptides
title_sort amaranth proteins emulsions as delivery system of angiotensin-i converting enzyme inhibitory peptides
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/152335
work_keys_str_mv AT suarezsantiagoemmanuel amaranthproteinsemulsionsasdeliverysystemofangiotensiniconvertingenzymeinhibitorypeptides
AT anonmariacristina amaranthproteinsemulsionsasdeliverysystemofangiotensiniconvertingenzymeinhibitorypeptides
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