Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis
Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after P...
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Autores principales: | , , , , , , , , |
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Formato: | Articulo |
Lenguaje: | Inglés |
Publicado: |
2020
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/145951 |
Aporte de: |
id |
I19-R120-10915-145951 |
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record_format |
dspace |
institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
collection |
SEDICI (UNLP) |
language |
Inglés |
topic |
Biología Ciencias Médicas Ocular toxoplasmosis Cytokine release |
spellingShingle |
Biología Ciencias Médicas Ocular toxoplasmosis Cytokine release Rudzinski, Marcelo Pardini, Lais Luján Bernstein, Mariana Moré, Gastón Andrés Khoury, Marina Duarte, Silvana Carolina Argüelles, Carina Francisca Reina, Silvia Oubiña, Jose Raul Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis |
topic_facet |
Biología Ciencias Médicas Ocular toxoplasmosis Cytokine release |
description |
Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high- or low-/non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL. |
format |
Articulo Articulo |
author |
Rudzinski, Marcelo Pardini, Lais Luján Bernstein, Mariana Moré, Gastón Andrés Khoury, Marina Duarte, Silvana Carolina Argüelles, Carina Francisca Reina, Silvia Oubiña, Jose Raul |
author_facet |
Rudzinski, Marcelo Pardini, Lais Luján Bernstein, Mariana Moré, Gastón Andrés Khoury, Marina Duarte, Silvana Carolina Argüelles, Carina Francisca Reina, Silvia Oubiña, Jose Raul |
author_sort |
Rudzinski, Marcelo |
title |
Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis |
title_short |
Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis |
title_full |
Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis |
title_fullStr |
Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis |
title_full_unstemmed |
Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis |
title_sort |
interferon-γ and il-10 release assay for patients with ocular toxoplasmosis |
publishDate |
2020 |
url |
http://sedici.unlp.edu.ar/handle/10915/145951 |
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Repositorios |
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