Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis

Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after P...

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Autores principales: Rudzinski, Marcelo, Pardini, Lais Luján, Bernstein, Mariana, Moré, Gastón Andrés, Khoury, Marina, Duarte, Silvana Carolina, Argüelles, Carina Francisca, Reina, Silvia, Oubiña, Jose Raul
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Biología
Ciencias Médicas
Ocular toxoplasmosis
Cytokine release
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/145951
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-145951
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Ciencias Médicas
Ocular toxoplasmosis
Cytokine release
spellingShingle Biología
Ciencias Médicas
Ocular toxoplasmosis
Cytokine release
Rudzinski, Marcelo
Pardini, Lais Luján
Bernstein, Mariana
Moré, Gastón Andrés
Khoury, Marina
Duarte, Silvana Carolina
Argüelles, Carina Francisca
Reina, Silvia
Oubiña, Jose Raul
Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis
topic_facet Biología
Ciencias Médicas
Ocular toxoplasmosis
Cytokine release
description Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high- or low-/non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL.
format Articulo
Articulo
author Rudzinski, Marcelo
Pardini, Lais Luján
Bernstein, Mariana
Moré, Gastón Andrés
Khoury, Marina
Duarte, Silvana Carolina
Argüelles, Carina Francisca
Reina, Silvia
Oubiña, Jose Raul
author_facet Rudzinski, Marcelo
Pardini, Lais Luján
Bernstein, Mariana
Moré, Gastón Andrés
Khoury, Marina
Duarte, Silvana Carolina
Argüelles, Carina Francisca
Reina, Silvia
Oubiña, Jose Raul
author_sort Rudzinski, Marcelo
title Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis
title_short Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis
title_full Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis
title_fullStr Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis
title_full_unstemmed Interferon-γ and IL-10 release assay for patients with ocular toxoplasmosis
title_sort interferon-γ and il-10 release assay for patients with ocular toxoplasmosis
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/145951
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