Vitamin A inhibits lipoperoxidation ascorbate-Fe⁺⁺ dependent of rat kidney microsomes and mitochondria
In the present study it was investigated if Vitamin A supplementation could protect rat kidney microsomes and mitochondria from in vitro lipoperoxidation. After incubation of rat kidney microsomes and mitochondria in an ascorbate-Fe⁺⁺ system, at 37°C during 60 min, it was observed that the total cpm...
Guardado en:
| Autores principales: | , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
1996
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/145820 |
| Aporte de: |
| id |
I19-R120-10915-145820 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Bioquímica Lipoperoxidation Microsomes Mitochondria Rat kidney |
| spellingShingle |
Bioquímica Lipoperoxidation Microsomes Mitochondria Rat kidney Piergiacomi, Viviana Angélica Palacios, Alejandro Catalá, Ángel Vitamin A inhibits lipoperoxidation ascorbate-Fe⁺⁺ dependent of rat kidney microsomes and mitochondria |
| topic_facet |
Bioquímica Lipoperoxidation Microsomes Mitochondria Rat kidney |
| description |
In the present study it was investigated if Vitamin A supplementation could protect rat kidney microsomes and mitochondria from in vitro lipoperoxidation. After incubation of rat kidney microsomes and mitochondria in an ascorbate-Fe⁺⁺ system, at 37°C during 60 min, it was observed that the total cpm/mg protein originated from light emission (chemiluminescence) was lower in those organelles obtained from the control group when compared with the vitamin A supplemented group. The fatty acid composition of microsomes and mitochondria from control group was profoundly modified when subjected to nonenzymatic lipoperoxidation with a considerable decrease of arachidonic acid, C20:4 (n-6) and docosapentaenoic acid, C22:5 (n−3) in mitochondria and docosahexaenoic acid C22:6 (n-3) in microsomes.As a consequence the peroxidizability index, a parameter based on the maximal rate of oxidation of specific fatty acids was higher in the supplemented animals than in those used as control. These results indicate that Vitamin A may act as antioxidant protecting rat kidney microsomes and mitochondria from deleterious effect. |
| format |
Articulo Articulo |
| author |
Piergiacomi, Viviana Angélica Palacios, Alejandro Catalá, Ángel |
| author_facet |
Piergiacomi, Viviana Angélica Palacios, Alejandro Catalá, Ángel |
| author_sort |
Piergiacomi, Viviana Angélica |
| title |
Vitamin A inhibits lipoperoxidation ascorbate-Fe⁺⁺ dependent of rat kidney microsomes and mitochondria |
| title_short |
Vitamin A inhibits lipoperoxidation ascorbate-Fe⁺⁺ dependent of rat kidney microsomes and mitochondria |
| title_full |
Vitamin A inhibits lipoperoxidation ascorbate-Fe⁺⁺ dependent of rat kidney microsomes and mitochondria |
| title_fullStr |
Vitamin A inhibits lipoperoxidation ascorbate-Fe⁺⁺ dependent of rat kidney microsomes and mitochondria |
| title_full_unstemmed |
Vitamin A inhibits lipoperoxidation ascorbate-Fe⁺⁺ dependent of rat kidney microsomes and mitochondria |
| title_sort |
vitamin a inhibits lipoperoxidation ascorbate-fe⁺⁺ dependent of rat kidney microsomes and mitochondria |
| publishDate |
1996 |
| url |
http://sedici.unlp.edu.ar/handle/10915/145820 |
| work_keys_str_mv |
AT piergiacomivivianaangelica vitaminainhibitslipoperoxidationascorbatefedependentofratkidneymicrosomesandmitochondria AT palaciosalejandro vitaminainhibitslipoperoxidationascorbatefedependentofratkidneymicrosomesandmitochondria AT catalaangel vitaminainhibitslipoperoxidationascorbatefedependentofratkidneymicrosomesandmitochondria |
| bdutipo_str |
Repositorios |
| _version_ |
1764820461697368065 |