Antimicrobial Efficacy of UV Radiation on Escherichia coli O157:H7 (EDL 933) in Fruit Juices of Different Absorptivities

The efficacy of UV light for inactivating E. coli (ATCC 25922) and E. coli O157:H7 (EDL 933) was examined in fruit juices (orange, apple, and multifruit) with different absorptivities under several operating conditions (liquid film thickness and agitation rate). The juices were inoculated with two b...

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Autores principales: Oteiza, Juan Martín, Peltzer, Mercedes Ana, Giannuzzi, Leda, Zaritzky, Noemí Elisabet
Formato: Articulo
Lenguaje:Inglés
Publicado: 2005
Materias:
Química
Física
Escherichia coli
UV Radiation
Fruit juices
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/143200
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-143200
record_format dspace
spelling I19-R120-10915-1432002023-05-02T13:11:20Z http://sedici.unlp.edu.ar/handle/10915/143200 issn:0362-028X issn:1944-9097 Antimicrobial Efficacy of UV Radiation on Escherichia coli O157:H7 (EDL 933) in Fruit Juices of Different Absorptivities Oteiza, Juan Martín Peltzer, Mercedes Ana Giannuzzi, Leda Zaritzky, Noemí Elisabet 2005-01-01 2022-10-03T16:28:37Z en Química Física Escherichia coli UV Radiation Fruit juices The efficacy of UV light for inactivating E. coli (ATCC 25922) and E. coli O157:H7 (EDL 933) was examined in fruit juices (orange, apple, and multifruit) with different absorptivities under several operating conditions (liquid film thickness and agitation rate). The juices were inoculated with two bacterial concentrations (10(5) and 10(7) CFU/ml) and were treated using a UV desinfection unit at 254 nm; UV doses ranged from 0 to 6 J/cm2. The effect of the culture medium, tryptone soy agar (TSA) and sorbitol MacConkey agar (SMAC), on the recovery of E. coli strains exposed to UV radiation was also analyzed. The most suitable culture medium for recovery of E. coli strains in juices exposed to UV radiation was TSA. Values of D (radiation dose [joules per square centimeter] necessary to decrease the microbial population by 90%) obtained in all juices assessed were higher in TSA than in SMAC. In the juices analyzed, stirring of the medium exposed to UV radiation and reducing liquid film thickness (to 0.7 mm) produced the highest bactericidal effect. A linear relationship was found between the D-values obtained and the absorptivity coefficients for all the juices. The higher the absorbance of the medium, the greater the values of D required to inactivate E. coli strains by UV radiation. An equation was developed to describe the relationship of the fraction of energy absorbed by the system (absorbed energy factor [AEF]), the thickness of the film exposed to UV radiation, and the absorptivity coefficient of the juices. A linear relationship was found between D and AEF in the different juices tested. Centro de Investigación y Desarrollo en Criotecnología de Alimentos Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 49-58
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Física
Escherichia coli
UV Radiation
Fruit juices
spellingShingle Química
Física
Escherichia coli
UV Radiation
Fruit juices
Oteiza, Juan Martín
Peltzer, Mercedes Ana
Giannuzzi, Leda
Zaritzky, Noemí Elisabet
Antimicrobial Efficacy of UV Radiation on Escherichia coli O157:H7 (EDL 933) in Fruit Juices of Different Absorptivities
topic_facet Química
Física
Escherichia coli
UV Radiation
Fruit juices
description The efficacy of UV light for inactivating E. coli (ATCC 25922) and E. coli O157:H7 (EDL 933) was examined in fruit juices (orange, apple, and multifruit) with different absorptivities under several operating conditions (liquid film thickness and agitation rate). The juices were inoculated with two bacterial concentrations (10(5) and 10(7) CFU/ml) and were treated using a UV desinfection unit at 254 nm; UV doses ranged from 0 to 6 J/cm2. The effect of the culture medium, tryptone soy agar (TSA) and sorbitol MacConkey agar (SMAC), on the recovery of E. coli strains exposed to UV radiation was also analyzed. The most suitable culture medium for recovery of E. coli strains in juices exposed to UV radiation was TSA. Values of D (radiation dose [joules per square centimeter] necessary to decrease the microbial population by 90%) obtained in all juices assessed were higher in TSA than in SMAC. In the juices analyzed, stirring of the medium exposed to UV radiation and reducing liquid film thickness (to 0.7 mm) produced the highest bactericidal effect. A linear relationship was found between the D-values obtained and the absorptivity coefficients for all the juices. The higher the absorbance of the medium, the greater the values of D required to inactivate E. coli strains by UV radiation. An equation was developed to describe the relationship of the fraction of energy absorbed by the system (absorbed energy factor [AEF]), the thickness of the film exposed to UV radiation, and the absorptivity coefficient of the juices. A linear relationship was found between D and AEF in the different juices tested.
format Articulo
Articulo
author Oteiza, Juan Martín
Peltzer, Mercedes Ana
Giannuzzi, Leda
Zaritzky, Noemí Elisabet
author_facet Oteiza, Juan Martín
Peltzer, Mercedes Ana
Giannuzzi, Leda
Zaritzky, Noemí Elisabet
author_sort Oteiza, Juan Martín
title Antimicrobial Efficacy of UV Radiation on Escherichia coli O157:H7 (EDL 933) in Fruit Juices of Different Absorptivities
title_short Antimicrobial Efficacy of UV Radiation on Escherichia coli O157:H7 (EDL 933) in Fruit Juices of Different Absorptivities
title_full Antimicrobial Efficacy of UV Radiation on Escherichia coli O157:H7 (EDL 933) in Fruit Juices of Different Absorptivities
title_fullStr Antimicrobial Efficacy of UV Radiation on Escherichia coli O157:H7 (EDL 933) in Fruit Juices of Different Absorptivities
title_full_unstemmed Antimicrobial Efficacy of UV Radiation on Escherichia coli O157:H7 (EDL 933) in Fruit Juices of Different Absorptivities
title_sort antimicrobial efficacy of uv radiation on escherichia coli o157:h7 (edl 933) in fruit juices of different absorptivities
publishDate 2005
url http://sedici.unlp.edu.ar/handle/10915/143200
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AT giannuzzileda antimicrobialefficacyofuvradiationonescherichiacolio157h7edl933infruitjuicesofdifferentabsorptivities
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