Lactobacillus plantarum CIDCA 8327: an α-glucan producing-strainisolated from kefir grains

Lactobacillus plantarum CIDCA 8327 is an exopolysaccharide (EPS)-producer strain isolated from kefir withpromising properties for the development of functional foods. The aim of the present study was to char-acterize the structure of the EPS synthesized by this strain grown in skim milk or semidefin...

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Autores principales: Gangoiti, María Virginia, Puertas, A. I., Hamet, María Fernanda, Peruzzo, Pablo José, Llamas, M.G., Medrano, Micaela, Prieto, A., Dueñas, M.T., Abraham, Analía Graciela
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/119462
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id I19-R120-10915-119462
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Lactobacillus plantarum
Kefir
Exopolysaccharides
Glucans
spellingShingle Química
Lactobacillus plantarum
Kefir
Exopolysaccharides
Glucans
Gangoiti, María Virginia
Puertas, A. I.
Hamet, María Fernanda
Peruzzo, Pablo José
Llamas, M.G.
Medrano, Micaela
Prieto, A.
Dueñas, M.T.
Abraham, Analía Graciela
Lactobacillus plantarum CIDCA 8327: an α-glucan producing-strainisolated from kefir grains
topic_facet Química
Lactobacillus plantarum
Kefir
Exopolysaccharides
Glucans
description Lactobacillus plantarum CIDCA 8327 is an exopolysaccharide (EPS)-producer strain isolated from kefir withpromising properties for the development of functional foods. The aim of the present study was to char-acterize the structure of the EPS synthesized by this strain grown in skim milk or semidefined medium(SDM). Additionally, genes involved in EPS synthesis were detected by PCR. L. plantarum produces anEPS with a molecular weight of 104Da in both media. When grown in SDM produce an heteropolysac-charide composed mainly of glucose, glucosamine and rhamnose meanwhile the EPS produced in milkwas composed exclusively of glucose indicating the influence of the sugar source. FTIR spectra of thisEPS showed signals attributable to an a-glucan. Both by1H NMR and methylation analysis it was pos-sible to determine that this polysaccharide is a branched α-(1 → 4)-d-glucan composed of 80% linear α-(1 → 4)-d-glucopyranosyl units and 19% (1 → 4)-d-glucopyranosyl units substituted at O-3 by single α-D-glucopyranosil residues.
format Articulo
Articulo
author Gangoiti, María Virginia
Puertas, A. I.
Hamet, María Fernanda
Peruzzo, Pablo José
Llamas, M.G.
Medrano, Micaela
Prieto, A.
Dueñas, M.T.
Abraham, Analía Graciela
author_facet Gangoiti, María Virginia
Puertas, A. I.
Hamet, María Fernanda
Peruzzo, Pablo José
Llamas, M.G.
Medrano, Micaela
Prieto, A.
Dueñas, M.T.
Abraham, Analía Graciela
author_sort Gangoiti, María Virginia
title Lactobacillus plantarum CIDCA 8327: an α-glucan producing-strainisolated from kefir grains
title_short Lactobacillus plantarum CIDCA 8327: an α-glucan producing-strainisolated from kefir grains
title_full Lactobacillus plantarum CIDCA 8327: an α-glucan producing-strainisolated from kefir grains
title_fullStr Lactobacillus plantarum CIDCA 8327: an α-glucan producing-strainisolated from kefir grains
title_full_unstemmed Lactobacillus plantarum CIDCA 8327: an α-glucan producing-strainisolated from kefir grains
title_sort lactobacillus plantarum cidca 8327: an α-glucan producing-strainisolated from kefir grains
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/119462
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