Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems
Whey protein concentrate (WPC) was fractionated by using hydroxypropylmethylcellulose (HPMC) at pH 6.5. Incompatible mixtures with different proportions of HPMC and WPC were prepared. After phase separation, the protein concentration in both phases was determined by the Kjeldahl method and the propo...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
2011
|
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 10238caa a22007937a 4500 | ||
|---|---|---|---|
| 001 | PAPER-10649 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204042.0 | ||
| 008 | 190411s2011 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-77957759552 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a FOHYE | ||
| 100 | 1 | |a Jara, F. | |
| 245 | 1 | 0 | |a Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems |
| 260 | |c 2011 | ||
| 270 | 1 | 0 | |m Pilosof, A.M.R.; Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria (1428), Buenos Aires, Argentina; email: apilosof@di.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Albertsson, P.A., Cajarville, A., Brooks, D.E., Tjerneld, F., Partition of proteins in aqueous polymer two-phase systems and the effect of molecular weight of the polymer (1987) Biochimica et Biophysica Acta, 926, pp. 87-93 | ||
| 504 | |a (1995) AOAC (Association of Official Analytical Chemists) Official methods of analysis, , AOAC Press, Arlington | ||
| 504 | |a Alomirah, H.F., Alli, I., Separation and characterization of β-lactoglobulin and α-lactalbumin from whey and whey protein preparations (2004) International Dairy Journal, 14, pp. 411-419 | ||
| 504 | |a Boaglio, A., Bassani, G., Picó, G., Nerli, B., Features of the milk whey protein partitioning in polyethyleneglycol-sodium citrate aqueous two-phase systems with the goal of isolating human alpha-1 antitrypsin expressed in bovine milk (2006) Journal of Chromatography B: Biomedical Sciences and Applications, 837, pp. 18-23 | ||
| 504 | |a Bolognese, B., Nerli, B., Picó, G., Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification (2005) Journal of Chromatography B: Biomedical Sciences and Applications, 814, pp. 347-353 | ||
| 504 | |a Capezio, L., Romanini, D., Picó, G.A., Nerli, B., Partition of whey milk proteins in aqueous two-phase systems of polyethylene glycol-phosphate as a starting point to isolate proteins expressed in transgenic milk (2005) Journal of Chromatography B: Biomedical Sciences and Applications, 819, pp. 25-31 | ||
| 504 | |a Cheang, B., Zydney, A.L., A two-stage ultrafiltration process for fractionation of whey protein isolate (2004) Journal of Membrane Science, 231, pp. 159-167 | ||
| 504 | |a Christian, T.J., Manley-Harris, M., Richards, G.N., A preliminary study of the use of larch arabinogalactan in aqueous two-phase systems (1998) Carbohydrate Polymers, 35, pp. 7-12 | ||
| 504 | |a Closs, C.B., Conde-Petit, B., Roberts, I.D., Tolstoguzov, V.B., Escher, F., Phase separation and rheology of aqueous starch/galactomannan systems (1999) Carbohydrate Polymers, 39, pp. 67-77 | ||
| 504 | |a da Silva, L.H., Meirelles, A.J., Bovine serum albumin, α-lactoalbumin and β-lactoglobulin partitioning in polyethylene glycol/maltodextrin aqueous-two-phase systems (2000) Carbohydrate Polymers, 42, pp. 279-282 | ||
| 504 | |a de Oliveira, M.C., de Abreu Filho, M.A., de Alcântara Pessôa Filho, P., Phase equilibrium and protein partitioning in aqueous two-phase systems containing ammonium carbamate and block copolymers PEO-PPO-PEO (2007) Biochemical Engineering Journal, 37, pp. 311-318 | ||
| 504 | |a Franco, T.T., Andrews, A.T., Asenjo, J.A., Use of chemically modified proteins to study the effect of a single protein property on partitioning in aqueous two-phase systems: effect of surface charge (1996) Biotechnology and Bioengineering, 49 (3), pp. 309-315 | ||
| 504 | |a González-Martínez, C., Becerra, M., Cáfer, A., Albors, A., Carot, J., Chiralt, A., Influence of substituting milk powder for whey powder on yogurt quality (2002) Trends in Food Science and Technology, 13, pp. 334-340 | ||
| 504 | |a Johansson, G., Partitioning proteins (1985) Partitioning in aqueous-two-phase systems, pp. 161-198. , Academic Press, Orlando, H. Walter, D.E. Brooks, D. Fisher (Eds.) | ||
| 504 | |a Johansson, G., Walter, H., Partitioning and concentrating biomaterials in aqueous phase systems (2000) Microcompartmentation and phase separation in cytoplasm. International review of cytology, pp. 33-60. , Academic Press, San Diego, H. Walter, D.E. Brooks, P.A. Srere (Eds.) | ||
| 504 | |a Laemmli, U., Cleavage of structural proteins during assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685 | ||
| 504 | |a Liu, X., Powers, J., Swanson, B., Hill, H., Clark, S., Modification of whey protein concentrate hydrophobicity by high hydrostatic pressure (2005) Innovative Food Science and Emerging Technologies, 6, pp. 310-317 | ||
| 504 | |a Pérez, O.E., Wargon, V., Pilosof, A.M.R., Gelation and structural characteristics of incompatible whey proteins/hydroxypropylmethylcellulose mixtures (2006) Food Hydrocolloids, 20 (7), pp. 966-974 | ||
| 504 | |a Pérez, O.E., Carrera Sánchez, C., Pilosof, A.M.R., Rodríguez Patino, J.M., Kinetics of adsorption of whey proteins and hydroxypropyl-methyl-cellulose mixtures at the air-water interface (2009) Journal of Colloid and Interface Science, 336 (2), pp. 485-496 | ||
| 504 | |a Picó, G., Romanini, D., Nerli, B., Farruggia, B., Polyethyleneglycol molecular mass and polydispersivity effect on protein partitioning in aqueous two-phase systems (2006) Journal of Chromatography B: Biomedical Sciences and Applications, 830, pp. 286-292 | ||
| 504 | |a Polyakov, V.I., Grinberg, V.Y., Tolstoguzov, V.B., Thermodynamic incompatibility of proteins (1997) Food Hydrocolloids, 11, pp. 171-180 | ||
| 504 | |a Reed, Z.H., Park, J.W., Qualification and quantification of fish protein in prepared Surimi crabstick (2008) Journal of Food Science, 73, pp. 329-334 | ||
| 504 | |a Rito-Palomares, M., Practical application of aqueous two-phase partition to process development for the recovery of biological products (2004) Journal of Chromatography B: Biomedical Sciences and Applications, 807, pp. 3-11 | ||
| 504 | |a Sarubbo, L.A., Oliveira, L.A., Porto, A.L.F., Duarte, H.S., Carneiro-Leão, A.M.A., Lima-Filho, J.L., New aqueous two-phase system based on cashew-nut tree gum and poly(ethylene glycol) (2000) Journal of Chromatography B, 743, pp. 79-84 | ||
| 504 | |a Schorsch, C., Jones, M.G., Norton, I.T., Thermodynamic incompatibility and microstructure of milk protein/locust bean gum/sucrose systems (1999) Food Hydrocolloids, 13, pp. 89-99 | ||
| 504 | |a Simonet, F., Garnier, C., Doublier, J.L., Partition of proteins in the aqueous guar/dextran two-phase system (2000) Food Hydrocolloids, 14, pp. 591-600 | ||
| 504 | |a Sivars, U., Tjerneld, F., Mechanisms of phase behaviour and protein partitioning in detergent/polymer aqueous two-phase systems for purification of integral membrane proteins (2000) Biochimica et Biophysica Acta, 1474, pp. 133-146 | ||
| 504 | |a Tolstoguzov, V., Some thermodynamic considerations in food formulation (2003) Food Hydrocolloids, 17, pp. 1-23 | ||
| 504 | |a Turgeon, S.L., Beaulieu, M., Schmitt, C., Sanchez, C., Protein-polisaccharide interactions: phase ordering kinetic, thermodynamic and structural aspect (2003) Current Opinion in Colloid and Interfaces Science, 8, pp. 401-414 | ||
| 504 | |a Zaslavsky, B.Y., (1995) Aqueous two-phase partitioning: Physical chemistry and bioanalytical applications, , Marcel Dekker, New York | ||
| 520 | 3 | |a Whey protein concentrate (WPC) was fractionated by using hydroxypropylmethylcellulose (HPMC) at pH 6.5. Incompatible mixtures with different proportions of HPMC and WPC were prepared. After phase separation, the protein concentration in both phases was determined by the Kjeldahl method and the proportion of each protein by SDS-PAGE combined with image analysis. The results show that the low molecular weight proteins α-lactalbumin (α-lac) and β-lactoglobulin (β-lg) were retained in high proportion in the upper phase (about 90% compared to 64% of WPC). The most efficient condition to fractionate β-lg and α-lac was the phase separation of an incompatible mixed system with a high initial concentration of WPC and a low initial concentration of HPMC i.e., WPC 20%, wt/wt/HPMC 0.5%, w/w. It can be concluded that the thermodynamic incompatibility which arises from mixing WPC with HPMC could be used as a method for fractionation of whey proteins. © 2010 Elsevier Ltd. |l eng | |
| 536 | |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica | ||
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: The authors acknowledge the support from the Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), and Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT). | ||
| 593 | |a Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria (1428), Buenos Aires, Argentina | ||
| 593 | |a Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina | ||
| 690 | 1 | 0 | |a HYDROXYPROPYLMETHYLCELLULOSE |
| 690 | 1 | 0 | |a PHASE SEPARATION |
| 690 | 1 | 0 | |a PROTEIN PARTITIONING |
| 690 | 1 | 0 | |a THERMODYNAMIC INCOMPATIBILITY |
| 690 | 1 | 0 | |a WHEY PROTEINS |
| 700 | 1 | |a Pilosof, A.M.R. | |
| 773 | 0 | |d 2011 |g v. 25 |h pp. 374-380 |k n. 3 |p Food Hydrocolloids |x 0268005X |w (AR-BaUEN)CENRE-4766 |t Food Hydrocolloids | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-77957759552&doi=10.1016%2fj.foodhyd.2010.07.003&partnerID=40&md5=51925e09d2ca7bffab25376d0204baec |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.foodhyd.2010.07.003 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_0268005X_v25_n3_p374_Jara |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v25_n3_p374_Jara |y Registro en la Biblioteca Digital |
| 961 | |a paper_0268005X_v25_n3_p374_Jara |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
| 999 | |c 71602 | ||