Efecto de la sobreexpresión de distintas isoformas de la Proteína Quinasa C (PKC) en la modulación del fenotipo maligno de células mamarias epiteliales. Alteración en la sensibilidad al tratamiento con retinoides

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Breast cancer represents the most common kind of cancer in women and it is estimatedthat more than one million new cases are diagnosed each year worldwide. It is a complexdisease, which presents a variety of subgroups with different genetic and histopathologicfeatures and therefore with different re...

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Detalles Bibliográficos
Autor principal: Díaz Bessone, María Inés
Formato: Tesis Doctoral
Lenguaje:Español
Publicado: 2016
Materias:
CANCER DE MAMA
PROTEINA QUINASA C
ACIDO RETINOICO
BREAST CANCER
PROTEIN KINASE C
RETINOIC ACID
Acceso en línea:https://hdl.handle.net/20.500.12110/tesis_n6034_DiazBessone
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:tesis_n6034_DiazBessone
record_format dspace
spelling todo:tesis_n6034_DiazBessone2023-10-03T13:04:10Z Efecto de la sobreexpresión de distintas isoformas de la Proteína Quinasa C (PKC) en la modulación del fenotipo maligno de células mamarias epiteliales. Alteración en la sensibilidad al tratamiento con retinoides Effect of overexpression of different isoforms of Protein Kinase C (PKC) in modulation of malignant phenotype of mammary ephitelial cells. Alteration in sensitivity to treatment with retinoids Díaz Bessone, María Inés CANCER DE MAMA PROTEINA QUINASA C ACIDO RETINOICO BREAST CANCER PROTEIN KINASE C RETINOIC ACID Breast cancer represents the most common kind of cancer in women and it is estimatedthat more than one million new cases are diagnosed each year worldwide. It is a complexdisease, which presents a variety of subgroups with different genetic and histopathologicfeatures and therefore with different responses to treatments. Multiple signaling pathways are involved in malignant progression. Some of them arepromising targets for therapeutic intervention, including protein kinase C (PKC), involvedin cellular events such as proliferation, differentiation and apoptosis and the retinoidsystem, widely implicated in cell differentiation. Since different mammary malignancies exhibit differential expression of PKC isoforms, wehave developed (human and murine) mammary cell models overexpressing α or δ PKCisoforms. Using these models we studied how PKCα or PKCδ alters cell parametersassociated with tumor progression and metastatic dissemination while we assessed theresponse to treatment with retinoids (ATRA). Our results suggest that PKCα overexpression confers to the cells a retinoic acid-sensitivephenotype, through an arrest in the G0 / G1 phase of the cell cycle. Overexpression of PKCδ, in MDA-MB231 cells, induced a less aggressive phenotype, significantly reducing itsinvasive capability. Finally the lack of response of this cell line to ATRA treatment could bedue to the inability to trans-repress AP-1 sites. Fil: Díaz Bessone, María Inés. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 Tesis Doctoral PDF Español info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar https://hdl.handle.net/20.500.12110/tesis_n6034_DiazBessone
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Español
orig_language_str_mv Español
topic CANCER DE MAMA
PROTEINA QUINASA C
ACIDO RETINOICO
BREAST CANCER
PROTEIN KINASE C
RETINOIC ACID
spellingShingle CANCER DE MAMA
PROTEINA QUINASA C
ACIDO RETINOICO
BREAST CANCER
PROTEIN KINASE C
RETINOIC ACID
Díaz Bessone, María Inés
Efecto de la sobreexpresión de distintas isoformas de la Proteína Quinasa C (PKC) en la modulación del fenotipo maligno de células mamarias epiteliales. Alteración en la sensibilidad al tratamiento con retinoides
topic_facet CANCER DE MAMA
PROTEINA QUINASA C
ACIDO RETINOICO
BREAST CANCER
PROTEIN KINASE C
RETINOIC ACID
description Breast cancer represents the most common kind of cancer in women and it is estimatedthat more than one million new cases are diagnosed each year worldwide. It is a complexdisease, which presents a variety of subgroups with different genetic and histopathologicfeatures and therefore with different responses to treatments. Multiple signaling pathways are involved in malignant progression. Some of them arepromising targets for therapeutic intervention, including protein kinase C (PKC), involvedin cellular events such as proliferation, differentiation and apoptosis and the retinoidsystem, widely implicated in cell differentiation. Since different mammary malignancies exhibit differential expression of PKC isoforms, wehave developed (human and murine) mammary cell models overexpressing α or δ PKCisoforms. Using these models we studied how PKCα or PKCδ alters cell parametersassociated with tumor progression and metastatic dissemination while we assessed theresponse to treatment with retinoids (ATRA). Our results suggest that PKCα overexpression confers to the cells a retinoic acid-sensitivephenotype, through an arrest in the G0 / G1 phase of the cell cycle. Overexpression of PKCδ, in MDA-MB231 cells, induced a less aggressive phenotype, significantly reducing itsinvasive capability. Finally the lack of response of this cell line to ATRA treatment could bedue to the inability to trans-repress AP-1 sites.
format Tesis Doctoral
author Díaz Bessone, María Inés
author_facet Díaz Bessone, María Inés
author_sort Díaz Bessone, María Inés
title Efecto de la sobreexpresión de distintas isoformas de la Proteína Quinasa C (PKC) en la modulación del fenotipo maligno de células mamarias epiteliales. Alteración en la sensibilidad al tratamiento con retinoides
title_short Efecto de la sobreexpresión de distintas isoformas de la Proteína Quinasa C (PKC) en la modulación del fenotipo maligno de células mamarias epiteliales. Alteración en la sensibilidad al tratamiento con retinoides
title_full Efecto de la sobreexpresión de distintas isoformas de la Proteína Quinasa C (PKC) en la modulación del fenotipo maligno de células mamarias epiteliales. Alteración en la sensibilidad al tratamiento con retinoides
title_fullStr Efecto de la sobreexpresión de distintas isoformas de la Proteína Quinasa C (PKC) en la modulación del fenotipo maligno de células mamarias epiteliales. Alteración en la sensibilidad al tratamiento con retinoides
title_full_unstemmed Efecto de la sobreexpresión de distintas isoformas de la Proteína Quinasa C (PKC) en la modulación del fenotipo maligno de células mamarias epiteliales. Alteración en la sensibilidad al tratamiento con retinoides
title_sort efecto de la sobreexpresión de distintas isoformas de la proteína quinasa c (pkc) en la modulación del fenotipo maligno de células mamarias epiteliales. alteración en la sensibilidad al tratamiento con retinoides
publishDate 2016
url https://hdl.handle.net/20.500.12110/tesis_n6034_DiazBessone
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AT diazbessonemariaines effectofoverexpressionofdifferentisoformsofproteinkinasecpkcinmodulationofmalignantphenotypeofmammaryephitelialcellsalterationinsensitivitytotreatmentwithretinoids
_version_ 1807316716353486848

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