Transcriptional features of fractionated human breast tumor chromatin
By means of controlled mechanical shearing it was possible to fractionate human breast tumor chromatin in 'active' and 'inactive' species, according to their in vitro template activity, using E. coli RNA polymerase. The 'active' molecules can be resolved in 3 well defin...
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_NIS18558_v29_n1_p81_Thierbach |
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todo:paper_NIS18558_v29_n1_p81_Thierbach2023-10-03T16:46:04Z Transcriptional features of fractionated human breast tumor chromatin Thierbach, L.I.A. Rosemblit, N. Pahn, C.R. Baldi, A. rna rna polymerase breast breast cancer chromatin escherichia coli euchromatin genetic transcription heterochromatin human cell in vitro study theoretical study Breast Neoplasms Chromatin Deoxyribonucleoproteins DNA, Neoplasm Female Human RNA Polymerase I RNA, Neoplasm Support, Non-U.S. Gov't Transcription, Genetic By means of controlled mechanical shearing it was possible to fractionate human breast tumor chromatin in 'active' and 'inactive' species, according to their in vitro template activity, using E. coli RNA polymerase. The 'active' molecules can be resolved in 3 well defined regions in an exponential sucrose gradient, showing approximately 300 times the efficiency for synthesizing ribonucleic acid, relative to the chromatin which migrated fastest. This technique could provide the initial tool to isolate eu-and heterochromatin from native human chromatin. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_NIS18558_v29_n1_p81_Thierbach |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
rna rna polymerase breast breast cancer chromatin escherichia coli euchromatin genetic transcription heterochromatin human cell in vitro study theoretical study Breast Neoplasms Chromatin Deoxyribonucleoproteins DNA, Neoplasm Female Human RNA Polymerase I RNA, Neoplasm Support, Non-U.S. Gov't Transcription, Genetic |
| spellingShingle |
rna rna polymerase breast breast cancer chromatin escherichia coli euchromatin genetic transcription heterochromatin human cell in vitro study theoretical study Breast Neoplasms Chromatin Deoxyribonucleoproteins DNA, Neoplasm Female Human RNA Polymerase I RNA, Neoplasm Support, Non-U.S. Gov't Transcription, Genetic Thierbach, L.I.A. Rosemblit, N. Pahn, C.R. Baldi, A. Transcriptional features of fractionated human breast tumor chromatin |
| topic_facet |
rna rna polymerase breast breast cancer chromatin escherichia coli euchromatin genetic transcription heterochromatin human cell in vitro study theoretical study Breast Neoplasms Chromatin Deoxyribonucleoproteins DNA, Neoplasm Female Human RNA Polymerase I RNA, Neoplasm Support, Non-U.S. Gov't Transcription, Genetic |
| description |
By means of controlled mechanical shearing it was possible to fractionate human breast tumor chromatin in 'active' and 'inactive' species, according to their in vitro template activity, using E. coli RNA polymerase. The 'active' molecules can be resolved in 3 well defined regions in an exponential sucrose gradient, showing approximately 300 times the efficiency for synthesizing ribonucleic acid, relative to the chromatin which migrated fastest. This technique could provide the initial tool to isolate eu-and heterochromatin from native human chromatin. |
| format |
JOUR |
| author |
Thierbach, L.I.A. Rosemblit, N. Pahn, C.R. Baldi, A. |
| author_facet |
Thierbach, L.I.A. Rosemblit, N. Pahn, C.R. Baldi, A. |
| author_sort |
Thierbach, L.I.A. |
| title |
Transcriptional features of fractionated human breast tumor chromatin |
| title_short |
Transcriptional features of fractionated human breast tumor chromatin |
| title_full |
Transcriptional features of fractionated human breast tumor chromatin |
| title_fullStr |
Transcriptional features of fractionated human breast tumor chromatin |
| title_full_unstemmed |
Transcriptional features of fractionated human breast tumor chromatin |
| title_sort |
transcriptional features of fractionated human breast tumor chromatin |
| url |
http://hdl.handle.net/20.500.12110/paper_NIS18558_v29_n1_p81_Thierbach |
| work_keys_str_mv |
AT thierbachlia transcriptionalfeaturesoffractionatedhumanbreasttumorchromatin AT rosemblitn transcriptionalfeaturesoffractionatedhumanbreasttumorchromatin AT pahncr transcriptionalfeaturesoffractionatedhumanbreasttumorchromatin AT baldia transcriptionalfeaturesoffractionatedhumanbreasttumorchromatin |
| _version_ |
1807316374766223360 |