Acute exposure of the freshwater snail planorbarius corneus to an environmental concentration of chlorpyrifos: Recovery studies of enzyme activities in gonads and effects on reproduction

Chlorpyrifos is an organophosphate insecticide widely used in a variety of food crops in Argentina and other countries. In a previous work, we have reported that subchronic exposure (14 days) to an environmental concentration of chlorpyrifos (5 μg L- 1 ) caused severe effects on reproduction of the...

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Autores principales: Rivadeneira, P.R., Agrelo, M., Kristoff, G.
Formato: CHAP
Materias:
Carboxylesterases
Chlorpyrifos
Cholinesterases
Gastropods
Reproduction
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_97816348_v_n_p27_Rivadeneira
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_97816348_v_n_p27_Rivadeneira2023-10-03T16:44:41Z Acute exposure of the freshwater snail planorbarius corneus to an environmental concentration of chlorpyrifos: Recovery studies of enzyme activities in gonads and effects on reproduction Rivadeneira, P.R. Agrelo, M. Kristoff, G. Carboxylesterases Chlorpyrifos Cholinesterases Gastropods Reproduction Chlorpyrifos is an organophosphate insecticide widely used in a variety of food crops in Argentina and other countries. In a previous work, we have reported that subchronic exposure (14 days) to an environmental concentration of chlorpyrifos (5 μg L- 1 ) caused severe effects on reproduction of the freshwater hermaphrodite gastropod Planorbarius corneus. The aim of the present work was to study the effects of chlorpyrifos on the reproduction of this adult snail after an acute (2 days) exposure. Also B-esterase activities (cholinesterases and carboxylesterases) were measured in the gonads, to study the possible link of the biochemical responses to the reproduction effects. Snails were exposed during 2 days to 5 μg L-1 of chlorpyrifos. Water control and solvent control (acetone) group were included in the bioassay. After 2 days of exposure, some snails per group were used to measured enzyme activities and other snails were transferred to water. Then, the bioassay was continued for 14 days. Snails were fed once a week. During the 14 days in water, the number of egg masses was registered and, for each egg mass, the number of egg masses without eggs, the number of eggs per mass, the time for hatching, the hatchings and the survival of the offspring were counted. After 2 days of exposure and after 2 and 14 days in water, snails were placed on ice, the shells were removed and gonad homogenates were realized. Supernatant fraction (6 for each time and for each group) was used to measure cholinesterase and carboxylesterase activities and protein content. After an acute exposure, 5 μg L-1 of chlorpyrifos caused a decrease on cholinesterase activity using acetylthiocholine as substrate (45 %) and carboxylesterase activities (26 % using p-nitrophenyl acetate as substrate and 40 % using p-nitrophenyl butyrate) in gonads. After 14 days in water, no recovery of cholinesterase activity was observed. However, carboxylesterases measured with both substrates returned to control values. Regarding the reproduction effects, only an increase in the number of egg masses without eggs and an increase in the time for hatching were observed. Recovery of carboxylesterases in gonads could be related to the lower effects observed on reproduction in comparison with the subchronic exposure. After 14 days of exposure, 5 μg L-1 of chlorpyrifos had produced a strongly decrease in B-esterase activities and severe effects on reproduction, such as the decreases in the % of hatching and survival of the offspring, the increases in egg masses without eggs and in the time of hatching. Exposures to environmental concentrations of chlorpyrifos caused different toxic effects on the reproduction of Planorbarius corneus depending on the time of exposure. © 2015 by Nova Science Publishers, Inc. All rights reserved. CHAP info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_97816348_v_n_p27_Rivadeneira
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Carboxylesterases
Chlorpyrifos
Cholinesterases
Gastropods
Reproduction
spellingShingle Carboxylesterases
Chlorpyrifos
Cholinesterases
Gastropods
Reproduction
Rivadeneira, P.R.
Agrelo, M.
Kristoff, G.
Acute exposure of the freshwater snail planorbarius corneus to an environmental concentration of chlorpyrifos: Recovery studies of enzyme activities in gonads and effects on reproduction
topic_facet Carboxylesterases
Chlorpyrifos
Cholinesterases
Gastropods
Reproduction
description Chlorpyrifos is an organophosphate insecticide widely used in a variety of food crops in Argentina and other countries. In a previous work, we have reported that subchronic exposure (14 days) to an environmental concentration of chlorpyrifos (5 μg L- 1 ) caused severe effects on reproduction of the freshwater hermaphrodite gastropod Planorbarius corneus. The aim of the present work was to study the effects of chlorpyrifos on the reproduction of this adult snail after an acute (2 days) exposure. Also B-esterase activities (cholinesterases and carboxylesterases) were measured in the gonads, to study the possible link of the biochemical responses to the reproduction effects. Snails were exposed during 2 days to 5 μg L-1 of chlorpyrifos. Water control and solvent control (acetone) group were included in the bioassay. After 2 days of exposure, some snails per group were used to measured enzyme activities and other snails were transferred to water. Then, the bioassay was continued for 14 days. Snails were fed once a week. During the 14 days in water, the number of egg masses was registered and, for each egg mass, the number of egg masses without eggs, the number of eggs per mass, the time for hatching, the hatchings and the survival of the offspring were counted. After 2 days of exposure and after 2 and 14 days in water, snails were placed on ice, the shells were removed and gonad homogenates were realized. Supernatant fraction (6 for each time and for each group) was used to measure cholinesterase and carboxylesterase activities and protein content. After an acute exposure, 5 μg L-1 of chlorpyrifos caused a decrease on cholinesterase activity using acetylthiocholine as substrate (45 %) and carboxylesterase activities (26 % using p-nitrophenyl acetate as substrate and 40 % using p-nitrophenyl butyrate) in gonads. After 14 days in water, no recovery of cholinesterase activity was observed. However, carboxylesterases measured with both substrates returned to control values. Regarding the reproduction effects, only an increase in the number of egg masses without eggs and an increase in the time for hatching were observed. Recovery of carboxylesterases in gonads could be related to the lower effects observed on reproduction in comparison with the subchronic exposure. After 14 days of exposure, 5 μg L-1 of chlorpyrifos had produced a strongly decrease in B-esterase activities and severe effects on reproduction, such as the decreases in the % of hatching and survival of the offspring, the increases in egg masses without eggs and in the time of hatching. Exposures to environmental concentrations of chlorpyrifos caused different toxic effects on the reproduction of Planorbarius corneus depending on the time of exposure. © 2015 by Nova Science Publishers, Inc. All rights reserved.
format CHAP
author Rivadeneira, P.R.
Agrelo, M.
Kristoff, G.
author_facet Rivadeneira, P.R.
Agrelo, M.
Kristoff, G.
author_sort Rivadeneira, P.R.
title Acute exposure of the freshwater snail planorbarius corneus to an environmental concentration of chlorpyrifos: Recovery studies of enzyme activities in gonads and effects on reproduction
title_short Acute exposure of the freshwater snail planorbarius corneus to an environmental concentration of chlorpyrifos: Recovery studies of enzyme activities in gonads and effects on reproduction
title_full Acute exposure of the freshwater snail planorbarius corneus to an environmental concentration of chlorpyrifos: Recovery studies of enzyme activities in gonads and effects on reproduction
title_fullStr Acute exposure of the freshwater snail planorbarius corneus to an environmental concentration of chlorpyrifos: Recovery studies of enzyme activities in gonads and effects on reproduction
title_full_unstemmed Acute exposure of the freshwater snail planorbarius corneus to an environmental concentration of chlorpyrifos: Recovery studies of enzyme activities in gonads and effects on reproduction
title_sort acute exposure of the freshwater snail planorbarius corneus to an environmental concentration of chlorpyrifos: recovery studies of enzyme activities in gonads and effects on reproduction
url http://hdl.handle.net/20.500.12110/paper_97816348_v_n_p27_Rivadeneira
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AT agrelom acuteexposureofthefreshwatersnailplanorbariuscorneustoanenvironmentalconcentrationofchlorpyrifosrecoverystudiesofenzymeactivitiesingonadsandeffectsonreproduction
AT kristoffg acuteexposureofthefreshwatersnailplanorbariuscorneustoanenvironmentalconcentrationofchlorpyrifosrecoverystudiesofenzymeactivitiesingonadsandeffectsonreproduction
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