Inorganic hydrogels for whole-culture encapsulation
Sol-gel encapsulation of living cells within inorganic hydrogels, mainly silica, is a promising technology for the design of biosensors. These host-guest functional materials maintain specific biologic functions of their guest while the properties of the host can be tuned to fulfill the requirements...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_97816346_v_n_p57_Perullini |
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todo:paper_97816346_v_n_p57_Perullini2023-10-03T16:44:37Z Inorganic hydrogels for whole-culture encapsulation Perullini, M. Spedalieri, C. Jobbdgv, M. Bilmes, S.A. Biosensors Ecotoxicity Sol-gel Two-step procedure Whole-culture encapsulation Sol-gel encapsulation of living cells within inorganic hydrogels, mainly silica, is a promising technology for the design of biosensors. These host-guest functional materials maintain specific biologic functions of their guest while the properties of the host can be tuned to fulfill the requirements of particular applications. Inorganic immobilization hosts exhibit several advantages over their (bio)polymer-based counterparts. While both hosts provide tailored porosity, the former offers enhanced chemical stability towards biodegradation as well as higher physical stability (low swelling). However, inone-pot encapsulations, the direct contact of cells with precursors during the sol-gel synthesis and the constraints imposed by the inorganic matrix during operating conditions may influence the biological response. In order to prevent this, an alternative two-step procedure was proposed. Living cells are pre-encapsulated in biocompatible carriers based on biopolymers such as alginates that confer protection during the inorganic and more cytotoxic synthesis. By means of these carriers, whole cultures of microorganisms remain confined in small liquid volumes generated inside the inorganic host, providing near conventional liquid culture conditions. Moreover, this approach allows the encapsulation of multicellular organisms and the co-encapsulation of multiple isolated cultures within a single common monolithic host, creating an artificial ecosystem in a diminished scale isolated inside a nanoporous matrix that would allow ecotoxicity studies to be carried out in portable devices for on-line and in situ pollution level assessment. © 2015 by Nova Science Publishers, Inc. Fil:Perullini, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Spedalieri, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. CHAP info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_97816346_v_n_p57_Perullini |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biosensors Ecotoxicity Sol-gel Two-step procedure Whole-culture encapsulation |
| spellingShingle |
Biosensors Ecotoxicity Sol-gel Two-step procedure Whole-culture encapsulation Perullini, M. Spedalieri, C. Jobbdgv, M. Bilmes, S.A. Inorganic hydrogels for whole-culture encapsulation |
| topic_facet |
Biosensors Ecotoxicity Sol-gel Two-step procedure Whole-culture encapsulation |
| description |
Sol-gel encapsulation of living cells within inorganic hydrogels, mainly silica, is a promising technology for the design of biosensors. These host-guest functional materials maintain specific biologic functions of their guest while the properties of the host can be tuned to fulfill the requirements of particular applications. Inorganic immobilization hosts exhibit several advantages over their (bio)polymer-based counterparts. While both hosts provide tailored porosity, the former offers enhanced chemical stability towards biodegradation as well as higher physical stability (low swelling). However, inone-pot encapsulations, the direct contact of cells with precursors during the sol-gel synthesis and the constraints imposed by the inorganic matrix during operating conditions may influence the biological response. In order to prevent this, an alternative two-step procedure was proposed. Living cells are pre-encapsulated in biocompatible carriers based on biopolymers such as alginates that confer protection during the inorganic and more cytotoxic synthesis. By means of these carriers, whole cultures of microorganisms remain confined in small liquid volumes generated inside the inorganic host, providing near conventional liquid culture conditions. Moreover, this approach allows the encapsulation of multicellular organisms and the co-encapsulation of multiple isolated cultures within a single common monolithic host, creating an artificial ecosystem in a diminished scale isolated inside a nanoporous matrix that would allow ecotoxicity studies to be carried out in portable devices for on-line and in situ pollution level assessment. © 2015 by Nova Science Publishers, Inc. |
| format |
CHAP |
| author |
Perullini, M. Spedalieri, C. Jobbdgv, M. Bilmes, S.A. |
| author_facet |
Perullini, M. Spedalieri, C. Jobbdgv, M. Bilmes, S.A. |
| author_sort |
Perullini, M. |
| title |
Inorganic hydrogels for whole-culture encapsulation |
| title_short |
Inorganic hydrogels for whole-culture encapsulation |
| title_full |
Inorganic hydrogels for whole-culture encapsulation |
| title_fullStr |
Inorganic hydrogels for whole-culture encapsulation |
| title_full_unstemmed |
Inorganic hydrogels for whole-culture encapsulation |
| title_sort |
inorganic hydrogels for whole-culture encapsulation |
| url |
http://hdl.handle.net/20.500.12110/paper_97816346_v_n_p57_Perullini |
| work_keys_str_mv |
AT perullinim inorganichydrogelsforwholecultureencapsulation AT spedalieric inorganichydrogelsforwholecultureencapsulation AT jobbdgvm inorganichydrogelsforwholecultureencapsulation AT bilmessa inorganichydrogelsforwholecultureencapsulation |
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1807324677479071744 |