Improved ROS measurement in root hair cells

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Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress respon...

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Autores principales: Juárez, S.P.D., Mangano, S., Estevez, J.M.
Formato: CHAP
Materias:
2',7-Dihidro dicloro fluorescein
Root hair
ROS
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_97814939_v_n_p67_Juarez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_97814939_v_n_p67_Juarez
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spelling todo:paper_97814939_v_n_p67_Juarez2023-10-03T16:43:45Z Improved ROS measurement in root hair cells Juárez, S.P.D. Mangano, S. Estevez, J.M. 2',7-Dihidro dicloro fluorescein Root hair ROS Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress responses, and abscisic acid signaling in guard cells. In Arabidopsis, root hairs ROS also play a key role in root hair growth and they control the activity of calcium channels required for polar growth (Takeda et al. Science 319:1241-1244, 2008). The production of reactive oxygen species is under a specific molecular control in order to avoid detrimental side effects. Here we describe a protocol to detect ROS by oxidation of a derivative of fluorescein: 2',7-dihidro dicloro fluorescein (H2DCFDA). © Springer Science+Business Media New York 2015. All rights are reserved. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. CHAP info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_97814939_v_n_p67_Juarez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 2',7-Dihidro dicloro fluorescein
Root hair
ROS
spellingShingle 2',7-Dihidro dicloro fluorescein
Root hair
ROS
Juárez, S.P.D.
Mangano, S.
Estevez, J.M.
Improved ROS measurement in root hair cells
topic_facet 2',7-Dihidro dicloro fluorescein
Root hair
ROS
description Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress responses, and abscisic acid signaling in guard cells. In Arabidopsis, root hairs ROS also play a key role in root hair growth and they control the activity of calcium channels required for polar growth (Takeda et al. Science 319:1241-1244, 2008). The production of reactive oxygen species is under a specific molecular control in order to avoid detrimental side effects. Here we describe a protocol to detect ROS by oxidation of a derivative of fluorescein: 2',7-dihidro dicloro fluorescein (H2DCFDA). © Springer Science+Business Media New York 2015. All rights are reserved.
format CHAP
author Juárez, S.P.D.
Mangano, S.
Estevez, J.M.
author_facet Juárez, S.P.D.
Mangano, S.
Estevez, J.M.
author_sort Juárez, S.P.D.
title Improved ROS measurement in root hair cells
title_short Improved ROS measurement in root hair cells
title_full Improved ROS measurement in root hair cells
title_fullStr Improved ROS measurement in root hair cells
title_full_unstemmed Improved ROS measurement in root hair cells
title_sort improved ros measurement in root hair cells
url http://hdl.handle.net/20.500.12110/paper_97814939_v_n_p67_Juarez
work_keys_str_mv AT juarezspd improvedrosmeasurementinroothaircells
AT manganos improvedrosmeasurementinroothaircells
AT estevezjm improvedrosmeasurementinroothaircells
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