Small photostable photoswitchable quantum dots as nanotools for live cell imaging

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We recently reported a novel surface architecture for the generation of biocompatible and stable photoswitchable quantum dots (psQDs). The system is based on Förster Resonance Energy Transfer (FRET) from a QD donor to diheteroarylethenes photochromic (PC) acceptors (pcFRET). The PC molecules are lin...

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Autores principales: Diaz, S.A., Toscani, A.M., Arndt-Jovin, D.J., Jovin, T.M., Jares-Erijman, E.A.
Formato: CONF
Materias:
EGFR
FRET
Imaging
Photochromic
Quantum dots
Amphiphilic polymers
Carboxyl groups
Epidermal growth factor receptors
Live cell
Live-cell imaging
Nanotools
Novel surfaces
Over-expression
Photo-switchable
Photostable
Quantum Dot
Resonance energy transfer
Time-resolved fluorescence
Energy transfer
Fluidics
Imaging techniques
Medical imaging
Medical nanotechnology
Microcomputers
Nanotechnology
Photochromism
Semiconductor quantum dots
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_97814398_v3_n_p205_Diaz
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_97814398_v3_n_p205_Diaz
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spelling todo:paper_97814398_v3_n_p205_Diaz2023-10-03T16:43:13Z Small photostable photoswitchable quantum dots as nanotools for live cell imaging Diaz, S.A. Toscani, A.M. Arndt-Jovin, D.J. Jovin, T.M. Jares-Erijman, E.A. EGFR FRET Imaging Photochromic Quantum dots Amphiphilic polymers Carboxyl groups EGFR Epidermal growth factor receptors FRET Live cell Live-cell imaging Nanotools Novel surfaces Over-expression Photo-switchable Photochromic Photostable Quantum Dot Resonance energy transfer Time-resolved fluorescence Energy transfer Fluidics Imaging techniques Medical imaging Medical nanotechnology Microcomputers Nanotechnology Photochromism Semiconductor quantum dots We recently reported a novel surface architecture for the generation of biocompatible and stable photoswitchable quantum dots (psQDs). The system is based on Förster Resonance Energy Transfer (FRET) from a QD donor to diheteroarylethenes photochromic (PC) acceptors (pcFRET). The PC molecules are linked to an amphiphilic polymer that self-assembles, creating an interior lipophilic space, in which the hydrophobic PCs are included, and external facing carboxyl groups. psQDs retain the desirable properties of the original QDs; furthermore the brightness can be tailored by light. The modulation of emission monitored by steady-state and time-resolved fluorescence is 35-40%. The epidermal growth factor receptor (EGFR) is of far-reaching biomedical importance due to its overexpression and/or mutation in various types of cancer. Here, we demonstrate imaging of live cells exposed to psQDs targeted to the EGFR. Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. CONF info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_97814398_v3_n_p205_Diaz
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic EGFR
FRET
Imaging
Photochromic
Quantum dots
Amphiphilic polymers
Carboxyl groups
EGFR
Epidermal growth factor receptors
FRET
Live cell
Live-cell imaging
Nanotools
Novel surfaces
Over-expression
Photo-switchable
Photochromic
Photostable
Quantum Dot
Resonance energy transfer
Time-resolved fluorescence
Energy transfer
Fluidics
Imaging techniques
Medical imaging
Medical nanotechnology
Microcomputers
Nanotechnology
Photochromism
Semiconductor quantum dots
spellingShingle EGFR
FRET
Imaging
Photochromic
Quantum dots
Amphiphilic polymers
Carboxyl groups
EGFR
Epidermal growth factor receptors
FRET
Live cell
Live-cell imaging
Nanotools
Novel surfaces
Over-expression
Photo-switchable
Photochromic
Photostable
Quantum Dot
Resonance energy transfer
Time-resolved fluorescence
Energy transfer
Fluidics
Imaging techniques
Medical imaging
Medical nanotechnology
Microcomputers
Nanotechnology
Photochromism
Semiconductor quantum dots
Diaz, S.A.
Toscani, A.M.
Arndt-Jovin, D.J.
Jovin, T.M.
Jares-Erijman, E.A.
Small photostable photoswitchable quantum dots as nanotools for live cell imaging
topic_facet EGFR
FRET
Imaging
Photochromic
Quantum dots
Amphiphilic polymers
Carboxyl groups
EGFR
Epidermal growth factor receptors
FRET
Live cell
Live-cell imaging
Nanotools
Novel surfaces
Over-expression
Photo-switchable
Photochromic
Photostable
Quantum Dot
Resonance energy transfer
Time-resolved fluorescence
Energy transfer
Fluidics
Imaging techniques
Medical imaging
Medical nanotechnology
Microcomputers
Nanotechnology
Photochromism
Semiconductor quantum dots
description We recently reported a novel surface architecture for the generation of biocompatible and stable photoswitchable quantum dots (psQDs). The system is based on Förster Resonance Energy Transfer (FRET) from a QD donor to diheteroarylethenes photochromic (PC) acceptors (pcFRET). The PC molecules are linked to an amphiphilic polymer that self-assembles, creating an interior lipophilic space, in which the hydrophobic PCs are included, and external facing carboxyl groups. psQDs retain the desirable properties of the original QDs; furthermore the brightness can be tailored by light. The modulation of emission monitored by steady-state and time-resolved fluorescence is 35-40%. The epidermal growth factor receptor (EGFR) is of far-reaching biomedical importance due to its overexpression and/or mutation in various types of cancer. Here, we demonstrate imaging of live cells exposed to psQDs targeted to the EGFR.
format CONF
author Diaz, S.A.
Toscani, A.M.
Arndt-Jovin, D.J.
Jovin, T.M.
Jares-Erijman, E.A.
author_facet Diaz, S.A.
Toscani, A.M.
Arndt-Jovin, D.J.
Jovin, T.M.
Jares-Erijman, E.A.
author_sort Diaz, S.A.
title Small photostable photoswitchable quantum dots as nanotools for live cell imaging
title_short Small photostable photoswitchable quantum dots as nanotools for live cell imaging
title_full Small photostable photoswitchable quantum dots as nanotools for live cell imaging
title_fullStr Small photostable photoswitchable quantum dots as nanotools for live cell imaging
title_full_unstemmed Small photostable photoswitchable quantum dots as nanotools for live cell imaging
title_sort small photostable photoswitchable quantum dots as nanotools for live cell imaging
url http://hdl.handle.net/20.500.12110/paper_97814398_v3_n_p205_Diaz
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AT jovintm smallphotostablephotoswitchablequantumdotsasnanotoolsforlivecellimaging
AT jareserijmanea smallphotostablephotoswitchablequantumdotsasnanotoolsforlivecellimaging
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