Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography
Recombinant human growth hormone (rhGH) is used for the treatment of several pathologies, most of them related to growth. Although different expression systems can be used for its production, the milk from transgenic cows is one of the most interesting due to the high rhGH level achieved (5 g/L). We...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_87567938_v34_n4_p999_Saavedra |
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todo:paper_87567938_v34_n4_p999_Saavedra2023-10-03T16:42:36Z Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography Saavedra, S.L. Martínez Ceron, M.C. Giudicessi, S.L. Forno, G. Bosco, M.B. Marani, M.M. Erra-Balsells, R. Albericio, F. Cascone, O. Camperi, S.A. biologics downstream processing peptide ligands recombinant biopharmaceuticals solid-phase peptide synthesis transgenic cow milk Affinity chromatography Chelation Purification Synthesis (chemical) biologics Biopharmaceuticals Cow milk Downstream-processing Peptide ligand Solid phase peptide synthesis Peptides Recombinant human growth hormone (rhGH) is used for the treatment of several pathologies, most of them related to growth. Although different expression systems can be used for its production, the milk from transgenic cows is one of the most interesting due to the high rhGH level achieved (5 g/L). We have designed and synthesized short peptides (9 or 10 amino acid long) using Fmoc chemistry and studied their ability to purify rhGH from milk once immobilized on an agarose support. Using spiked milk with the hormone as a sample, rhGH was purified with 88% yield and 92% purity in a single step with a fold purification of 4.5. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:999–1005, 2018. © 2018 American Institute of Chemical Engineers JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_87567938_v34_n4_p999_Saavedra |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
biologics downstream processing peptide ligands recombinant biopharmaceuticals solid-phase peptide synthesis transgenic cow milk Affinity chromatography Chelation Purification Synthesis (chemical) biologics Biopharmaceuticals Cow milk Downstream-processing Peptide ligand Solid phase peptide synthesis Peptides |
| spellingShingle |
biologics downstream processing peptide ligands recombinant biopharmaceuticals solid-phase peptide synthesis transgenic cow milk Affinity chromatography Chelation Purification Synthesis (chemical) biologics Biopharmaceuticals Cow milk Downstream-processing Peptide ligand Solid phase peptide synthesis Peptides Saavedra, S.L. Martínez Ceron, M.C. Giudicessi, S.L. Forno, G. Bosco, M.B. Marani, M.M. Erra-Balsells, R. Albericio, F. Cascone, O. Camperi, S.A. Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography |
| topic_facet |
biologics downstream processing peptide ligands recombinant biopharmaceuticals solid-phase peptide synthesis transgenic cow milk Affinity chromatography Chelation Purification Synthesis (chemical) biologics Biopharmaceuticals Cow milk Downstream-processing Peptide ligand Solid phase peptide synthesis Peptides |
| description |
Recombinant human growth hormone (rhGH) is used for the treatment of several pathologies, most of them related to growth. Although different expression systems can be used for its production, the milk from transgenic cows is one of the most interesting due to the high rhGH level achieved (5 g/L). We have designed and synthesized short peptides (9 or 10 amino acid long) using Fmoc chemistry and studied their ability to purify rhGH from milk once immobilized on an agarose support. Using spiked milk with the hormone as a sample, rhGH was purified with 88% yield and 92% purity in a single step with a fold purification of 4.5. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:999–1005, 2018. © 2018 American Institute of Chemical Engineers |
| format |
JOUR |
| author |
Saavedra, S.L. Martínez Ceron, M.C. Giudicessi, S.L. Forno, G. Bosco, M.B. Marani, M.M. Erra-Balsells, R. Albericio, F. Cascone, O. Camperi, S.A. |
| author_facet |
Saavedra, S.L. Martínez Ceron, M.C. Giudicessi, S.L. Forno, G. Bosco, M.B. Marani, M.M. Erra-Balsells, R. Albericio, F. Cascone, O. Camperi, S.A. |
| author_sort |
Saavedra, S.L. |
| title |
Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography |
| title_short |
Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography |
| title_full |
Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography |
| title_fullStr |
Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography |
| title_full_unstemmed |
Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography |
| title_sort |
single step recombinant human growth hormone (rhgh) purification from milk by peptide affinity chromatography |
| url |
http://hdl.handle.net/20.500.12110/paper_87567938_v34_n4_p999_Saavedra |
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