Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures

ABSTRACT: Similar localizations of the Con A and wheat germ lectin receptors were obtained by using fluorescent lectins, in nonfixed spermatozoa and in spermatozoa fixed with formaldehyde and methanol, showing that in samples with the same previous treatment, worked out by the same operator, in whic...

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Autores principales: NARDONE, P., CEREZO, A.S., DE CEREZO, J.M.S.
Formato: JOUR
Materias:
cytochemical localization
human spermatozoa
oligomannosidic oligosaccharides
lectin
oligosaccharide
cytochemistry
cytology
human
male genital system
mannose oligosaccharide
oligosaccharide receptor
priority journal
spermatozoon
Comparative Study
Concanavalin A
Fixatives
Fluoresceins
Formaldehyde
Histocytochemistry
Human
Lectins
Male
Methanol
Receptors, Concanavalin A
Receptors, Mitogen
Spermatozoa
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_87558920_v9_n4_p124_NARDONE
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_87558920_v9_n4_p124_NARDONE
record_format dspace
spelling todo:paper_87558920_v9_n4_p124_NARDONE2023-10-03T16:42:31Z Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures NARDONE, P. CEREZO, A.S. DE CEREZO, J.M.S. cytochemical localization human spermatozoa oligomannosidic oligosaccharides lectin oligosaccharide cytochemistry cytology human male genital system mannose oligosaccharide oligosaccharide receptor priority journal spermatozoon Comparative Study Concanavalin A Fixatives Fluoresceins Formaldehyde Histocytochemistry Human Lectins Male Methanol Receptors, Concanavalin A Receptors, Mitogen Spermatozoa Support, Non-U.S. Gov't ABSTRACT: Similar localizations of the Con A and wheat germ lectin receptors were obtained by using fluorescent lectins, in nonfixed spermatozoa and in spermatozoa fixed with formaldehyde and methanol, showing that in samples with the same previous treatment, worked out by the same operator, in which enough determinations have been performed to eliminate individual variations, the different procedures of fixation produced similar results. The localizations obtained with fluorescent lectins confirm previous results, produced with the peroxidase technique, indicating that the lectins interact with oligomannosidic oligosaccharide receptors situated mainly in the equatorial segment of the acrosome and postnu‐clear cap. They also indicate the presence of similar receptors that were not detected previously on the neck and intermediate segment. The larger size of the lectin‐peroxidase‐diaminebenzidine reagent compared to that of the fluorescent lectins suggests that the new receptors are semicriptic and were not detected by steric effects in the first case, but were able to interact with lower volume, fluorescent probes. It is suggested that these oligomannosidic chains could be recognition signals for the elimination of incompetent sperm during their passage through the female reproductive track. Also these oligosaccharides and its possible metabolic variations could be involved in the interaction between the acrosome‐reacted spermatozoa with the zone pellucida. 1985 Munksgaard Fil:CEREZO, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_87558920_v9_n4_p124_NARDONE
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic cytochemical localization
human spermatozoa
oligomannosidic oligosaccharides
lectin
oligosaccharide
cytochemistry
cytology
human
male genital system
mannose oligosaccharide
oligosaccharide receptor
priority journal
spermatozoon
Comparative Study
Concanavalin A
Fixatives
Fluoresceins
Formaldehyde
Histocytochemistry
Human
Lectins
Male
Methanol
Receptors, Concanavalin A
Receptors, Mitogen
Spermatozoa
Support, Non-U.S. Gov't
spellingShingle cytochemical localization
human spermatozoa
oligomannosidic oligosaccharides
lectin
oligosaccharide
cytochemistry
cytology
human
male genital system
mannose oligosaccharide
oligosaccharide receptor
priority journal
spermatozoon
Comparative Study
Concanavalin A
Fixatives
Fluoresceins
Formaldehyde
Histocytochemistry
Human
Lectins
Male
Methanol
Receptors, Concanavalin A
Receptors, Mitogen
Spermatozoa
Support, Non-U.S. Gov't
NARDONE, P.
CEREZO, A.S.
DE CEREZO, J.M.S.
Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures
topic_facet cytochemical localization
human spermatozoa
oligomannosidic oligosaccharides
lectin
oligosaccharide
cytochemistry
cytology
human
male genital system
mannose oligosaccharide
oligosaccharide receptor
priority journal
spermatozoon
Comparative Study
Concanavalin A
Fixatives
Fluoresceins
Formaldehyde
Histocytochemistry
Human
Lectins
Male
Methanol
Receptors, Concanavalin A
Receptors, Mitogen
Spermatozoa
Support, Non-U.S. Gov't
description ABSTRACT: Similar localizations of the Con A and wheat germ lectin receptors were obtained by using fluorescent lectins, in nonfixed spermatozoa and in spermatozoa fixed with formaldehyde and methanol, showing that in samples with the same previous treatment, worked out by the same operator, in which enough determinations have been performed to eliminate individual variations, the different procedures of fixation produced similar results. The localizations obtained with fluorescent lectins confirm previous results, produced with the peroxidase technique, indicating that the lectins interact with oligomannosidic oligosaccharide receptors situated mainly in the equatorial segment of the acrosome and postnu‐clear cap. They also indicate the presence of similar receptors that were not detected previously on the neck and intermediate segment. The larger size of the lectin‐peroxidase‐diaminebenzidine reagent compared to that of the fluorescent lectins suggests that the new receptors are semicriptic and were not detected by steric effects in the first case, but were able to interact with lower volume, fluorescent probes. It is suggested that these oligomannosidic chains could be recognition signals for the elimination of incompetent sperm during their passage through the female reproductive track. Also these oligosaccharides and its possible metabolic variations could be involved in the interaction between the acrosome‐reacted spermatozoa with the zone pellucida. 1985 Munksgaard
format JOUR
author NARDONE, P.
CEREZO, A.S.
DE CEREZO, J.M.S.
author_facet NARDONE, P.
CEREZO, A.S.
DE CEREZO, J.M.S.
author_sort NARDONE, P.
title Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures
title_short Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures
title_full Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures
title_fullStr Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures
title_full_unstemmed Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures
title_sort cytochemical characterization and localization of oligomannosidic oligosaccharide receptors on the normal human spermatozoa using fluorescent lectins: comparison of different fixation procedures
url http://hdl.handle.net/20.500.12110/paper_87558920_v9_n4_p124_NARDONE
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AT cerezoas cytochemicalcharacterizationandlocalizationofoligomannosidicoligosaccharidereceptorsonthenormalhumanspermatozoausingfluorescentlectinscomparisonofdifferentfixationprocedures
AT decerezojms cytochemicalcharacterizationandlocalizationofoligomannosidicoligosaccharidereceptorsonthenormalhumanspermatozoausingfluorescentlectinscomparisonofdifferentfixationprocedures
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