Effect of antisperm antibodies present in human follicular fluid upon the acrosome reaction and sperm-zona pellucida interaction
Problem: To determine the ability of IgGs isolated from follicular fluids (hFFIgGs) to induce the acrosome reaction (AR) in human spermatozoa and to inhibit sperm-zona pellucida (ZP) interaction. Method of study: Incubation of capacitated spermatozoa with hFFIgGs (n = 40) and assessment of their eff...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_87558920_v50_n3_p209_MarinBriggiler |
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todo:paper_87558920_v50_n3_p209_MarinBriggiler2023-10-03T16:42:31Z Effect of antisperm antibodies present in human follicular fluid upon the acrosome reaction and sperm-zona pellucida interaction Marín-Briggiler, C.I. Vazquez-Levin, M.H. Gonzalez-Echevarría, F. Blaquier, J.A. Miranda, P.V. Tezón, J.G. Acrosome reaction Antisperm antibodies Follicular fluid Spermatozoa Zona pellucida immunoglobulin G sperm antibody acrosome reaction adult antibody isolation article cell interaction clinical article controlled study correlation analysis exocytosis female fertilization in vitro human human cell immunoassay infertility inhibition kinetics intracytoplasmic sperm injection male ovary follicle fluid priority journal scoring system sperm donor spermatozoon capacitation Acrosome Reaction Adult Female Follicular Fluid Humans Immunoglobulin G Male Spermatozoa Zona Pellucida Problem: To determine the ability of IgGs isolated from follicular fluids (hFFIgGs) to induce the acrosome reaction (AR) in human spermatozoa and to inhibit sperm-zona pellucida (ZP) interaction. Method of study: Incubation of capacitated spermatozoa with hFFIgGs (n = 40) and assessment of their effect on the AR or hemizona (HZ) assay in a condition that allows sperm-ZP interaction, avoiding acrosomal exocytosis. Results: hFFIgGs from different women varied in their ability of inducing the AR. Those hFFIgGs with the highest AR-inducing capacity evoked the exocytotic response in most of the different sperm donors tested [high Induction Frequency (IF)]. Some of these antibodies were also able of inhibiting sperm binding to ZP [low HZ Index (HZI)]. A significant correlation was found between the IF and the HZI for each hFFIgG. Conclusions: Human follicular fluid contains antibodies capable of inducing the AR and inhibiting sperm-ZP binding, suggesting that they could be directed towards ZP receptors. hFFIgGs would constitute a tool for the identification of sperm entities involved in fertilization. © Blackwell Munksgaard, 2003. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_87558920_v50_n3_p209_MarinBriggiler |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acrosome reaction Antisperm antibodies Follicular fluid Spermatozoa Zona pellucida immunoglobulin G sperm antibody acrosome reaction adult antibody isolation article cell interaction clinical article controlled study correlation analysis exocytosis female fertilization in vitro human human cell immunoassay infertility inhibition kinetics intracytoplasmic sperm injection male ovary follicle fluid priority journal scoring system sperm donor spermatozoon capacitation Acrosome Reaction Adult Female Follicular Fluid Humans Immunoglobulin G Male Spermatozoa Zona Pellucida |
| spellingShingle |
Acrosome reaction Antisperm antibodies Follicular fluid Spermatozoa Zona pellucida immunoglobulin G sperm antibody acrosome reaction adult antibody isolation article cell interaction clinical article controlled study correlation analysis exocytosis female fertilization in vitro human human cell immunoassay infertility inhibition kinetics intracytoplasmic sperm injection male ovary follicle fluid priority journal scoring system sperm donor spermatozoon capacitation Acrosome Reaction Adult Female Follicular Fluid Humans Immunoglobulin G Male Spermatozoa Zona Pellucida Marín-Briggiler, C.I. Vazquez-Levin, M.H. Gonzalez-Echevarría, F. Blaquier, J.A. Miranda, P.V. Tezón, J.G. Effect of antisperm antibodies present in human follicular fluid upon the acrosome reaction and sperm-zona pellucida interaction |
| topic_facet |
Acrosome reaction Antisperm antibodies Follicular fluid Spermatozoa Zona pellucida immunoglobulin G sperm antibody acrosome reaction adult antibody isolation article cell interaction clinical article controlled study correlation analysis exocytosis female fertilization in vitro human human cell immunoassay infertility inhibition kinetics intracytoplasmic sperm injection male ovary follicle fluid priority journal scoring system sperm donor spermatozoon capacitation Acrosome Reaction Adult Female Follicular Fluid Humans Immunoglobulin G Male Spermatozoa Zona Pellucida |
| description |
Problem: To determine the ability of IgGs isolated from follicular fluids (hFFIgGs) to induce the acrosome reaction (AR) in human spermatozoa and to inhibit sperm-zona pellucida (ZP) interaction. Method of study: Incubation of capacitated spermatozoa with hFFIgGs (n = 40) and assessment of their effect on the AR or hemizona (HZ) assay in a condition that allows sperm-ZP interaction, avoiding acrosomal exocytosis. Results: hFFIgGs from different women varied in their ability of inducing the AR. Those hFFIgGs with the highest AR-inducing capacity evoked the exocytotic response in most of the different sperm donors tested [high Induction Frequency (IF)]. Some of these antibodies were also able of inhibiting sperm binding to ZP [low HZ Index (HZI)]. A significant correlation was found between the IF and the HZI for each hFFIgG. Conclusions: Human follicular fluid contains antibodies capable of inducing the AR and inhibiting sperm-ZP binding, suggesting that they could be directed towards ZP receptors. hFFIgGs would constitute a tool for the identification of sperm entities involved in fertilization. © Blackwell Munksgaard, 2003. |
| format |
JOUR |
| author |
Marín-Briggiler, C.I. Vazquez-Levin, M.H. Gonzalez-Echevarría, F. Blaquier, J.A. Miranda, P.V. Tezón, J.G. |
| author_facet |
Marín-Briggiler, C.I. Vazquez-Levin, M.H. Gonzalez-Echevarría, F. Blaquier, J.A. Miranda, P.V. Tezón, J.G. |
| author_sort |
Marín-Briggiler, C.I. |
| title |
Effect of antisperm antibodies present in human follicular fluid upon the acrosome reaction and sperm-zona pellucida interaction |
| title_short |
Effect of antisperm antibodies present in human follicular fluid upon the acrosome reaction and sperm-zona pellucida interaction |
| title_full |
Effect of antisperm antibodies present in human follicular fluid upon the acrosome reaction and sperm-zona pellucida interaction |
| title_fullStr |
Effect of antisperm antibodies present in human follicular fluid upon the acrosome reaction and sperm-zona pellucida interaction |
| title_full_unstemmed |
Effect of antisperm antibodies present in human follicular fluid upon the acrosome reaction and sperm-zona pellucida interaction |
| title_sort |
effect of antisperm antibodies present in human follicular fluid upon the acrosome reaction and sperm-zona pellucida interaction |
| url |
http://hdl.handle.net/20.500.12110/paper_87558920_v50_n3_p209_MarinBriggiler |
| work_keys_str_mv |
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1782026907850637312 |