Determination of IL-1 and IL-6 levels in human embryo culture-conditioned media
Problem: The aim of this study was to evaluate the presence of interleukin-1 (IL-1) and IL-6 in 24-hour human embryo culture-conditioned media (HECCM) and to find any embryo-related factor(s) to predict pregnancy during IVF procedure. Method: IL-1β and IL-6 levels were measured in 36 samples of HECC...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_87558920_v37_n2_p191_Baranao |
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todo:paper_87558920_v37_n2_p191_Baranao2023-10-03T16:42:31Z Determination of IL-1 and IL-6 levels in human embryo culture-conditioned media Barañao, R.I. Piazza, A. Rumi, L.S. De Fried, E.P. Human embryo cultured conditioned media (HECCM) IL-6 Interleukin-1 (IL-1) Viable pregnancies interleukin 1beta interleukin 6 adult article clinical article controlled study culture medium embryo embryo culture enzyme linked immunosorbent assay female fertilization in vitro human human cell nidation pregnancy priority journal Problem: The aim of this study was to evaluate the presence of interleukin-1 (IL-1) and IL-6 in 24-hour human embryo culture-conditioned media (HECCM) and to find any embryo-related factor(s) to predict pregnancy during IVF procedure. Method: IL-1β and IL-6 levels were measured in 36 samples of HECCM and 17 cell culture media that had not been exposed to embryos, which were used as controls. Both cytokine levels were measured by the ELISA technique, using commercially available kits. Results and Conclusions: We found an average IL-1β level ± SEM of 82 ± 4 pg/ml (n = 11) in HECCM from viable pregnancies and a significantly lower value, 14 ± 2 pg/ml (n = 25, P < 0.001), in HECCM from embryos that did not lead to viable pregnancies. In control media the average IL-1β level was 11 ± 1 pg/ml (n = 17), (P < 0.001 vs. HECCM from viable pregnancies). In contrast IL-6 levels were undetectable in all samples analyzed. Judging by our results we suggest that measurement of IL-1 level in HECCM could be a useful parameter for predicting implantation in techniques of assisted reproduction. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_87558920_v37_n2_p191_Baranao |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Human embryo cultured conditioned media (HECCM) IL-6 Interleukin-1 (IL-1) Viable pregnancies interleukin 1beta interleukin 6 adult article clinical article controlled study culture medium embryo embryo culture enzyme linked immunosorbent assay female fertilization in vitro human human cell nidation pregnancy priority journal |
| spellingShingle |
Human embryo cultured conditioned media (HECCM) IL-6 Interleukin-1 (IL-1) Viable pregnancies interleukin 1beta interleukin 6 adult article clinical article controlled study culture medium embryo embryo culture enzyme linked immunosorbent assay female fertilization in vitro human human cell nidation pregnancy priority journal Barañao, R.I. Piazza, A. Rumi, L.S. De Fried, E.P. Determination of IL-1 and IL-6 levels in human embryo culture-conditioned media |
| topic_facet |
Human embryo cultured conditioned media (HECCM) IL-6 Interleukin-1 (IL-1) Viable pregnancies interleukin 1beta interleukin 6 adult article clinical article controlled study culture medium embryo embryo culture enzyme linked immunosorbent assay female fertilization in vitro human human cell nidation pregnancy priority journal |
| description |
Problem: The aim of this study was to evaluate the presence of interleukin-1 (IL-1) and IL-6 in 24-hour human embryo culture-conditioned media (HECCM) and to find any embryo-related factor(s) to predict pregnancy during IVF procedure. Method: IL-1β and IL-6 levels were measured in 36 samples of HECCM and 17 cell culture media that had not been exposed to embryos, which were used as controls. Both cytokine levels were measured by the ELISA technique, using commercially available kits. Results and Conclusions: We found an average IL-1β level ± SEM of 82 ± 4 pg/ml (n = 11) in HECCM from viable pregnancies and a significantly lower value, 14 ± 2 pg/ml (n = 25, P < 0.001), in HECCM from embryos that did not lead to viable pregnancies. In control media the average IL-1β level was 11 ± 1 pg/ml (n = 17), (P < 0.001 vs. HECCM from viable pregnancies). In contrast IL-6 levels were undetectable in all samples analyzed. Judging by our results we suggest that measurement of IL-1 level in HECCM could be a useful parameter for predicting implantation in techniques of assisted reproduction. |
| format |
JOUR |
| author |
Barañao, R.I. Piazza, A. Rumi, L.S. De Fried, E.P. |
| author_facet |
Barañao, R.I. Piazza, A. Rumi, L.S. De Fried, E.P. |
| author_sort |
Barañao, R.I. |
| title |
Determination of IL-1 and IL-6 levels in human embryo culture-conditioned media |
| title_short |
Determination of IL-1 and IL-6 levels in human embryo culture-conditioned media |
| title_full |
Determination of IL-1 and IL-6 levels in human embryo culture-conditioned media |
| title_fullStr |
Determination of IL-1 and IL-6 levels in human embryo culture-conditioned media |
| title_full_unstemmed |
Determination of IL-1 and IL-6 levels in human embryo culture-conditioned media |
| title_sort |
determination of il-1 and il-6 levels in human embryo culture-conditioned media |
| url |
http://hdl.handle.net/20.500.12110/paper_87558920_v37_n2_p191_Baranao |
| work_keys_str_mv |
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