Carbohydrates of the Surface of the Normal Human Spermatozoon

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ABSTRACT: Autoproteolysis of human spermatozoa produces oligopeptides with oligosaccharide chains of the N‐glycosidic‐linked type that are released from the “surface exposed” parts of glycoproteins. The products eluted in the previous washing of the spermatozoa have the same composition and solubili...

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Autores principales: MAZZINI, M.N., CERACI, P., DE CEREZO, J.M.S., CEREZO, A.S.
Formato: JOUR
Materias:
autoproteolysis
human spermatozoa
N‐Glycosidic‐linked oligosaccharides
glycoprotein
receptor
biological model
human
human cell
male genital system
priority journal
protein degradation
spermatozoon
Carbohydrates
Cell Membrane
Human
Male
Membrane Proteins
Oligopeptides
Oligosaccharides
Spermatozoa
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_87558920_v11_n4_p107_MAZZINI
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_87558920_v11_n4_p107_MAZZINI
record_format dspace
spelling todo:paper_87558920_v11_n4_p107_MAZZINI2023-10-03T16:42:30Z Carbohydrates of the Surface of the Normal Human Spermatozoon MAZZINI, M.N. CERACI, P. DE CEREZO, J.M.S. CEREZO, A.S. autoproteolysis human spermatozoa N‐Glycosidic‐linked oligosaccharides glycoprotein receptor biological model human human cell male genital system priority journal protein degradation spermatozoon Carbohydrates Cell Membrane Human Male Membrane Proteins Oligopeptides Oligosaccharides Spermatozoa Support, Non-U.S. Gov't ABSTRACT: Autoproteolysis of human spermatozoa produces oligopeptides with oligosaccharide chains of the N‐glycosidic‐linked type that are released from the “surface exposed” parts of glycoproteins. The products eluted in the previous washing of the spermatozoa have the same composition and solubility characteristics as the oligopeptides from the digestion. This suggests that autoproteolysis is a constant process that normally occurs on the spermatozoa membrane. The cytochemical characterization and localization of the N‐glycosidic‐linked oligosaccharide receptors on the human spermatozoa membrane after digestion, in the presence or absence of seminal plasma, indicates that only part of the oligosaccharides are cleaved. Their distribution on the different zones of the spermatozoon changed as the probability of detecting these receptors in the intermediate segment increased after proteolysis; this indicates that in this zone the receptors are cryptic ones that become exposed by the action of the proteolytic enzymes. In the presence of seminal plasma most receptors on the acrosome are eliminated. 1986 Munksgaard Fil:MAZZINI, M.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:CEREZO, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_87558920_v11_n4_p107_MAZZINI
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic autoproteolysis
human spermatozoa
N‐Glycosidic‐linked oligosaccharides
glycoprotein
receptor
biological model
human
human cell
male genital system
priority journal
protein degradation
spermatozoon
Carbohydrates
Cell Membrane
Human
Male
Membrane Proteins
Oligopeptides
Oligosaccharides
Spermatozoa
Support, Non-U.S. Gov't
spellingShingle autoproteolysis
human spermatozoa
N‐Glycosidic‐linked oligosaccharides
glycoprotein
receptor
biological model
human
human cell
male genital system
priority journal
protein degradation
spermatozoon
Carbohydrates
Cell Membrane
Human
Male
Membrane Proteins
Oligopeptides
Oligosaccharides
Spermatozoa
Support, Non-U.S. Gov't
MAZZINI, M.N.
CERACI, P.
DE CEREZO, J.M.S.
CEREZO, A.S.
Carbohydrates of the Surface of the Normal Human Spermatozoon
topic_facet autoproteolysis
human spermatozoa
N‐Glycosidic‐linked oligosaccharides
glycoprotein
receptor
biological model
human
human cell
male genital system
priority journal
protein degradation
spermatozoon
Carbohydrates
Cell Membrane
Human
Male
Membrane Proteins
Oligopeptides
Oligosaccharides
Spermatozoa
Support, Non-U.S. Gov't
description ABSTRACT: Autoproteolysis of human spermatozoa produces oligopeptides with oligosaccharide chains of the N‐glycosidic‐linked type that are released from the “surface exposed” parts of glycoproteins. The products eluted in the previous washing of the spermatozoa have the same composition and solubility characteristics as the oligopeptides from the digestion. This suggests that autoproteolysis is a constant process that normally occurs on the spermatozoa membrane. The cytochemical characterization and localization of the N‐glycosidic‐linked oligosaccharide receptors on the human spermatozoa membrane after digestion, in the presence or absence of seminal plasma, indicates that only part of the oligosaccharides are cleaved. Their distribution on the different zones of the spermatozoon changed as the probability of detecting these receptors in the intermediate segment increased after proteolysis; this indicates that in this zone the receptors are cryptic ones that become exposed by the action of the proteolytic enzymes. In the presence of seminal plasma most receptors on the acrosome are eliminated. 1986 Munksgaard
format JOUR
author MAZZINI, M.N.
CERACI, P.
DE CEREZO, J.M.S.
CEREZO, A.S.
author_facet MAZZINI, M.N.
CERACI, P.
DE CEREZO, J.M.S.
CEREZO, A.S.
author_sort MAZZINI, M.N.
title Carbohydrates of the Surface of the Normal Human Spermatozoon
title_short Carbohydrates of the Surface of the Normal Human Spermatozoon
title_full Carbohydrates of the Surface of the Normal Human Spermatozoon
title_fullStr Carbohydrates of the Surface of the Normal Human Spermatozoon
title_full_unstemmed Carbohydrates of the Surface of the Normal Human Spermatozoon
title_sort carbohydrates of the surface of the normal human spermatozoon
url http://hdl.handle.net/20.500.12110/paper_87558920_v11_n4_p107_MAZZINI
work_keys_str_mv AT mazzinimn carbohydratesofthesurfaceofthenormalhumanspermatozoon
AT ceracip carbohydratesofthesurfaceofthenormalhumanspermatozoon
AT decerezojms carbohydratesofthesurfaceofthenormalhumanspermatozoon
AT cerezoas carbohydratesofthesurfaceofthenormalhumanspermatozoon
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