Susceptibility of placental mitochondria to oxidative stress
Background: Two different mitochondrial fractions (MFs) have been characterized in the human placenta: the “light” and “heavy” fractions (LMF and HMF). Although these organelles are the main source of reactive oxygen species, an imbalance between their production and the rate of detoxification repre...
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todo:paper_24721727_v110_n16_p1228_PapaGobbi2023-10-03T16:42:27Z Susceptibility of placental mitochondria to oxidative stress Papa Gobbi, R. Magnarelli, G. Rovedatti, M.G. mitochondria oxidative stress placenta reactive oxygen species catalase glutathione hydrogen peroxide malonaldehyde adult antioxidant activity Article cell isolation controlled study enzymatic assay enzyme activity female human human tissue lipid peroxidation mitochondrion normal human oxidation oxidative stress placenta protein carbonylation protein determination spectrophotometry Background: Two different mitochondrial fractions (MFs) have been characterized in the human placenta: the “light” and “heavy” fractions (LMF and HMF). Although these organelles are the main source of reactive oxygen species, an imbalance between their production and the rate of detoxification represents a serious threat to mitochondrial homeostasis and, in the case of the placenta, also to the fetus. The aim of this study was to evaluate the antioxidant capacity and susceptibility to oxidative stress in both types of MFs. Methods: Human MFs were isolated from healthy donors (n = 11) and either incubated or not with H2O2. Catalase (CAT) activity, and reduced glutathione (GSH), lipid peroxidation (LP), and protein carbonylation (PC) levels were determined. Results: H2O2 treatment increased LP and PC levels and decreased CAT activity. GSH levels were similar in control and treated MFs. Conclusion: H2O2 caused oxidative damage in both LMF and HMF and the antioxidant system measured in these two MFs responded similarly. To the best of our knowledge, this is the first partial description of the antioxidant defense in placental HMF and LMF performed in a cell-free assay. The small number of antioxidant system parameters measured did not allow detecting differences between HMF and LMF. © 2018 Wiley Periodicals, Inc. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_24721727_v110_n16_p1228_PapaGobbi |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
mitochondria oxidative stress placenta reactive oxygen species catalase glutathione hydrogen peroxide malonaldehyde adult antioxidant activity Article cell isolation controlled study enzymatic assay enzyme activity female human human tissue lipid peroxidation mitochondrion normal human oxidation oxidative stress placenta protein carbonylation protein determination spectrophotometry |
| spellingShingle |
mitochondria oxidative stress placenta reactive oxygen species catalase glutathione hydrogen peroxide malonaldehyde adult antioxidant activity Article cell isolation controlled study enzymatic assay enzyme activity female human human tissue lipid peroxidation mitochondrion normal human oxidation oxidative stress placenta protein carbonylation protein determination spectrophotometry Papa Gobbi, R. Magnarelli, G. Rovedatti, M.G. Susceptibility of placental mitochondria to oxidative stress |
| topic_facet |
mitochondria oxidative stress placenta reactive oxygen species catalase glutathione hydrogen peroxide malonaldehyde adult antioxidant activity Article cell isolation controlled study enzymatic assay enzyme activity female human human tissue lipid peroxidation mitochondrion normal human oxidation oxidative stress placenta protein carbonylation protein determination spectrophotometry |
| description |
Background: Two different mitochondrial fractions (MFs) have been characterized in the human placenta: the “light” and “heavy” fractions (LMF and HMF). Although these organelles are the main source of reactive oxygen species, an imbalance between their production and the rate of detoxification represents a serious threat to mitochondrial homeostasis and, in the case of the placenta, also to the fetus. The aim of this study was to evaluate the antioxidant capacity and susceptibility to oxidative stress in both types of MFs. Methods: Human MFs were isolated from healthy donors (n = 11) and either incubated or not with H2O2. Catalase (CAT) activity, and reduced glutathione (GSH), lipid peroxidation (LP), and protein carbonylation (PC) levels were determined. Results: H2O2 treatment increased LP and PC levels and decreased CAT activity. GSH levels were similar in control and treated MFs. Conclusion: H2O2 caused oxidative damage in both LMF and HMF and the antioxidant system measured in these two MFs responded similarly. To the best of our knowledge, this is the first partial description of the antioxidant defense in placental HMF and LMF performed in a cell-free assay. The small number of antioxidant system parameters measured did not allow detecting differences between HMF and LMF. © 2018 Wiley Periodicals, Inc. |
| format |
JOUR |
| author |
Papa Gobbi, R. Magnarelli, G. Rovedatti, M.G. |
| author_facet |
Papa Gobbi, R. Magnarelli, G. Rovedatti, M.G. |
| author_sort |
Papa Gobbi, R. |
| title |
Susceptibility of placental mitochondria to oxidative stress |
| title_short |
Susceptibility of placental mitochondria to oxidative stress |
| title_full |
Susceptibility of placental mitochondria to oxidative stress |
| title_fullStr |
Susceptibility of placental mitochondria to oxidative stress |
| title_full_unstemmed |
Susceptibility of placental mitochondria to oxidative stress |
| title_sort |
susceptibility of placental mitochondria to oxidative stress |
| url |
http://hdl.handle.net/20.500.12110/paper_24721727_v110_n16_p1228_PapaGobbi |
| work_keys_str_mv |
AT papagobbir susceptibilityofplacentalmitochondriatooxidativestress AT magnarellig susceptibilityofplacentalmitochondriatooxidativestress AT rovedattimg susceptibilityofplacentalmitochondriatooxidativestress |
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1807314386560221184 |