Cleavage of Peptides from Amphibian Skin Revealed by Combining Analysis of Gland Secretion and in Situ MALDI Imaging Mass Spectrometry

Peptides from skin secretions of amphibians are considered important components of their immune system and also play a relevant role in their defense mechanism against predators. Herein, by using mass spectrometry (MS), we characterize the sequence of 13 peptides from the gland secretion of the hyli...

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Autores principales: Brunetti, A.E., Marani, M.M., Soldi, R.A., Mendonça, J.N., Faivovich, J., Cabrera, G.M., Lopes, N.P.
Formato: JOUR
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_24701343_v3_n5_p5426_Brunetti
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_24701343_v3_n5_p5426_Brunetti2023-10-03T16:42:25Z Cleavage of Peptides from Amphibian Skin Revealed by Combining Analysis of Gland Secretion and in Situ MALDI Imaging Mass Spectrometry Brunetti, A.E. Marani, M.M. Soldi, R.A. Mendonça, J.N. Faivovich, J. Cabrera, G.M. Lopes, N.P. Peptides from skin secretions of amphibians are considered important components of their immune system and also play a relevant role in their defense mechanism against predators. Herein, by using mass spectrometry (MS), we characterize the sequence of 13 peptides from the gland secretion of the hylid tree frog, Boana punctata. Using in situ matrix-assisted laser desorption ionization imaging MS of a transverse section of the skin tissue, we show that some peptides are stored as longer molecules that are cleaved after being secreted, whereas others do not undergo any modification. Sequence comparison with peptides from other Boana species and analysis of the three-dimensional theoretical structure indicate that this cleavage depends on both the presence of a specific sequence motif and the secondary structure. The fact that peptides undergo a rapid cleavage upon secretion suggests that stored and secreted peptides may have distinct roles for anuran survival, including defense against pathogens and predators. Copyright © 2018 American Chemical Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_24701343_v3_n5_p5426_Brunetti
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description Peptides from skin secretions of amphibians are considered important components of their immune system and also play a relevant role in their defense mechanism against predators. Herein, by using mass spectrometry (MS), we characterize the sequence of 13 peptides from the gland secretion of the hylid tree frog, Boana punctata. Using in situ matrix-assisted laser desorption ionization imaging MS of a transverse section of the skin tissue, we show that some peptides are stored as longer molecules that are cleaved after being secreted, whereas others do not undergo any modification. Sequence comparison with peptides from other Boana species and analysis of the three-dimensional theoretical structure indicate that this cleavage depends on both the presence of a specific sequence motif and the secondary structure. The fact that peptides undergo a rapid cleavage upon secretion suggests that stored and secreted peptides may have distinct roles for anuran survival, including defense against pathogens and predators. Copyright © 2018 American Chemical Society.
format JOUR
author Brunetti, A.E.
Marani, M.M.
Soldi, R.A.
Mendonça, J.N.
Faivovich, J.
Cabrera, G.M.
Lopes, N.P.
spellingShingle Brunetti, A.E.
Marani, M.M.
Soldi, R.A.
Mendonça, J.N.
Faivovich, J.
Cabrera, G.M.
Lopes, N.P.
Cleavage of Peptides from Amphibian Skin Revealed by Combining Analysis of Gland Secretion and in Situ MALDI Imaging Mass Spectrometry
author_facet Brunetti, A.E.
Marani, M.M.
Soldi, R.A.
Mendonça, J.N.
Faivovich, J.
Cabrera, G.M.
Lopes, N.P.
author_sort Brunetti, A.E.
title Cleavage of Peptides from Amphibian Skin Revealed by Combining Analysis of Gland Secretion and in Situ MALDI Imaging Mass Spectrometry
title_short Cleavage of Peptides from Amphibian Skin Revealed by Combining Analysis of Gland Secretion and in Situ MALDI Imaging Mass Spectrometry
title_full Cleavage of Peptides from Amphibian Skin Revealed by Combining Analysis of Gland Secretion and in Situ MALDI Imaging Mass Spectrometry
title_fullStr Cleavage of Peptides from Amphibian Skin Revealed by Combining Analysis of Gland Secretion and in Situ MALDI Imaging Mass Spectrometry
title_full_unstemmed Cleavage of Peptides from Amphibian Skin Revealed by Combining Analysis of Gland Secretion and in Situ MALDI Imaging Mass Spectrometry
title_sort cleavage of peptides from amphibian skin revealed by combining analysis of gland secretion and in situ maldi imaging mass spectrometry
url http://hdl.handle.net/20.500.12110/paper_24701343_v3_n5_p5426_Brunetti
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