Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina

Neobalantidium coli (Pomajbíková et al., 2013) is a cosmopolitan ciliate which colonizes the intestine of humans and animals. Pigs are the most important host and reservoir for this parasite, although others mammals have been described. Humans can acquire the disease through the ingestion of water a...

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Autores principales:	López Arias, L., Guillemi, E., Bordoni, N., Farber, M., Garbossa, G.
Formato:	JOUR
Materias:	18S rRNA Human balantidiosis Neobalantidium coli PCR Swine balantidiosis Zoonotic transmission RNA 18S animal experiment animal health Argentina Article balantidiasis controlled study cyst disease transmission DNA extraction feces health care planning microscopy mortality nonhuman parasite pig polymerase chain reaction zoonosis
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_24059390_v10_n_p114_LopezArias
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_24059390_v10_n_p114_LopezArias
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spelling	todo:paper_24059390_v10_n_p114_LopezArias2023-10-03T16:41:24Z Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina López Arias, L. Guillemi, E. Bordoni, N. Farber, M. Garbossa, G. 18S rRNA Human balantidiosis Neobalantidium coli PCR Swine balantidiosis Zoonotic transmission RNA 18S animal experiment animal health Argentina Article balantidiasis controlled study cyst disease transmission DNA extraction feces health care planning microscopy mortality Neobalantidium coli nonhuman parasite pig polymerase chain reaction zoonosis Neobalantidium coli (Pomajbíková et al., 2013) is a cosmopolitan ciliate which colonizes the intestine of humans and animals. Pigs are the most important host and reservoir for this parasite, although others mammals have been described. Humans can acquire the disease through the ingestion of water and food contaminated with cysts and even from person to person contact. Farmers and slaughterhouse workers from rural areas of developing countries have an increased incidence of balantidiosis. In Argentina, despite swine production on family farms covers 70% of domestic consumption requirements; there is a lack of veterinary animal health planning which result in high rate of animal mortality, as well as environmental risk due to inefficient facilities and mismanagement of manure and effluents. At present there are no epidemiological data on balantidiosis in Argentina, except for isolated reports. Therefore, the aims of this study were to establish the frequency of N. coli in pigs raised under different conditions and to explore the zoonotic potential. In order to confirm the identity of Neobalantidium coli like-cysts founded in the feces, a set of N. coli specific primers based on 18S rRNA gene sequences was designed. The molecular identification of N. coli was performed in 88.9% (16 out of 18) of swine stool samples in which cysts had been visualized. The fecal samples obtained from pigs raised on more open farmland showed a lower percentage of N. coli than those obtained from animals raised in swine pens. On the other hand, molecular identification of N. coli was also performed in human feces. Pairwise comparison of sequences obtained from pigs and human fecal samples from the NW Region of Argentina showed a high percentage of similarity, indicating a possible zoonotic transmission. © 2017 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_24059390_v10_n_p114_LopezArias
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	18S rRNA Human balantidiosis Neobalantidium coli PCR Swine balantidiosis Zoonotic transmission RNA 18S animal experiment animal health Argentina Article balantidiasis controlled study cyst disease transmission DNA extraction feces health care planning microscopy mortality Neobalantidium coli nonhuman parasite pig polymerase chain reaction zoonosis
spellingShingle	18S rRNA Human balantidiosis Neobalantidium coli PCR Swine balantidiosis Zoonotic transmission RNA 18S animal experiment animal health Argentina Article balantidiasis controlled study cyst disease transmission DNA extraction feces health care planning microscopy mortality Neobalantidium coli nonhuman parasite pig polymerase chain reaction zoonosis López Arias, L. Guillemi, E. Bordoni, N. Farber, M. Garbossa, G. Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina
topic_facet	18S rRNA Human balantidiosis Neobalantidium coli PCR Swine balantidiosis Zoonotic transmission RNA 18S animal experiment animal health Argentina Article balantidiasis controlled study cyst disease transmission DNA extraction feces health care planning microscopy mortality Neobalantidium coli nonhuman parasite pig polymerase chain reaction zoonosis
description	Neobalantidium coli (Pomajbíková et al., 2013) is a cosmopolitan ciliate which colonizes the intestine of humans and animals. Pigs are the most important host and reservoir for this parasite, although others mammals have been described. Humans can acquire the disease through the ingestion of water and food contaminated with cysts and even from person to person contact. Farmers and slaughterhouse workers from rural areas of developing countries have an increased incidence of balantidiosis. In Argentina, despite swine production on family farms covers 70% of domestic consumption requirements; there is a lack of veterinary animal health planning which result in high rate of animal mortality, as well as environmental risk due to inefficient facilities and mismanagement of manure and effluents. At present there are no epidemiological data on balantidiosis in Argentina, except for isolated reports. Therefore, the aims of this study were to establish the frequency of N. coli in pigs raised under different conditions and to explore the zoonotic potential. In order to confirm the identity of Neobalantidium coli like-cysts founded in the feces, a set of N. coli specific primers based on 18S rRNA gene sequences was designed. The molecular identification of N. coli was performed in 88.9% (16 out of 18) of swine stool samples in which cysts had been visualized. The fecal samples obtained from pigs raised on more open farmland showed a lower percentage of N. coli than those obtained from animals raised in swine pens. On the other hand, molecular identification of N. coli was also performed in human feces. Pairwise comparison of sequences obtained from pigs and human fecal samples from the NW Region of Argentina showed a high percentage of similarity, indicating a possible zoonotic transmission. © 2017 Elsevier B.V.
format	JOUR
author	López Arias, L. Guillemi, E. Bordoni, N. Farber, M. Garbossa, G.
author_facet	López Arias, L. Guillemi, E. Bordoni, N. Farber, M. Garbossa, G.
author_sort	López Arias, L.
title	Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina
title_short	Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina
title_full	Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina
title_fullStr	Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina
title_full_unstemmed	Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina
title_sort	development of a pcr assay for identification of neobalantidium coli (pomajbíková et al., 2013) in argentina
url	http://hdl.handle.net/20.500.12110/paper_24059390_v10_n_p114_LopezArias
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Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina

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