Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)

Fulvia fulva (syn. Cladosporium fulvum, Mycosphaerellaceae) is a dematiaceous fungus that causes tomato leaf mould. It is characterized by its biotrophic lifestyle and the synthesis of the bianthraquinone secondary metabolite, cladofulvin. The aim of the study was to characterize the dark pigment ph...

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Autores principales: Medina, R., Lucentini, C.G., Franco, M.E.E., Petroselli, G., Rosso, J.A., Erra-Balsells, R., Balatti, P.A., Saparrat, M.C.N.
Formato: JOUR
Materias:
Biochemistry
Genetics
Microbiology
Molecular biology
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_24058440_v4_n12_p_Medina
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_24058440_v4_n12_p_Medina2023-10-03T16:41:23Z Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum) Medina, R. Lucentini, C.G. Franco, M.E.E. Petroselli, G. Rosso, J.A. Erra-Balsells, R. Balatti, P.A. Saparrat, M.C.N. Biochemistry Genetics Microbiology Molecular biology Fulvia fulva (syn. Cladosporium fulvum, Mycosphaerellaceae) is a dematiaceous fungus that causes tomato leaf mould. It is characterized by its biotrophic lifestyle and the synthesis of the bianthraquinone secondary metabolite, cladofulvin. The aim of the study was to characterize the dark pigment photochemically synthesized by F. fulva and to elucidate its biochemical pathway. We isolated a black pigment from in vitro cultures of the fungus. We determined the pigment to be 1,8-dihydroxynaphthalene (DHN)-melanin based on its chemical and photochemical characteristics, as well as the presence of flaviolin, when fungal reductases were inhibited by tricyclazole. Furthermore, the pks1 gene involved in pigment synthesis has a KS domain already associated with DHN-melanin. Our findings support the relevance of studying melanization in F. fulva. © 2018 The Authors JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_24058440_v4_n12_p_Medina
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Biochemistry
Genetics
Microbiology
Molecular biology
spellingShingle Biochemistry
Genetics
Microbiology
Molecular biology
Medina, R.
Lucentini, C.G.
Franco, M.E.E.
Petroselli, G.
Rosso, J.A.
Erra-Balsells, R.
Balatti, P.A.
Saparrat, M.C.N.
Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
topic_facet Biochemistry
Genetics
Microbiology
Molecular biology
description Fulvia fulva (syn. Cladosporium fulvum, Mycosphaerellaceae) is a dematiaceous fungus that causes tomato leaf mould. It is characterized by its biotrophic lifestyle and the synthesis of the bianthraquinone secondary metabolite, cladofulvin. The aim of the study was to characterize the dark pigment photochemically synthesized by F. fulva and to elucidate its biochemical pathway. We isolated a black pigment from in vitro cultures of the fungus. We determined the pigment to be 1,8-dihydroxynaphthalene (DHN)-melanin based on its chemical and photochemical characteristics, as well as the presence of flaviolin, when fungal reductases were inhibited by tricyclazole. Furthermore, the pks1 gene involved in pigment synthesis has a KS domain already associated with DHN-melanin. Our findings support the relevance of studying melanization in F. fulva. © 2018 The Authors
format JOUR
author Medina, R.
Lucentini, C.G.
Franco, M.E.E.
Petroselli, G.
Rosso, J.A.
Erra-Balsells, R.
Balatti, P.A.
Saparrat, M.C.N.
author_facet Medina, R.
Lucentini, C.G.
Franco, M.E.E.
Petroselli, G.
Rosso, J.A.
Erra-Balsells, R.
Balatti, P.A.
Saparrat, M.C.N.
author_sort Medina, R.
title Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_short Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_full Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_fullStr Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_full_unstemmed Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_sort identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of fulvia fulva (syn. cladosporium fulvum)
url http://hdl.handle.net/20.500.12110/paper_24058440_v4_n12_p_Medina
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