Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis
Species of the Drosophila melanogaster species subgroup, including the species D. simulans, D. mauritiana, D. yakuba, and D. santomea, have long served as model systems for studying evolution. However, studies in these species have been limited by a paucity of genetic and transgenic reagents. Here,...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_21601836_v7_n4_p1339_Stern |
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todo:paper_21601836_v7_n4_p1339_Stern2023-10-03T16:39:30Z Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis Stern, D.L. Crocker, J. Ding, Y. Frankel, N. Kappes, G. Kim, E. Kuzmickas, R. Lemire, A. Mast, J.D. Picard, S. Drosophila Evolution Genetics Speciation Transgenics φC31 integrase reagent yellow fluorescent protein enhanced green fluorescent protein green fluorescent protein Article controlled study Drosophila Drosophila mauritiana Drosophila santomea Drosophila simulans Drosophila virilis Drosophila yakuba gene expression genetic strain insect genetics nonhuman reporter gene transgene transposon allele animal Drosophila Drosophila simulans eye gene expression regulation genetics genomics metabolism species difference transgenic animal Alleles Animals Animals, Genetically Modified DNA Transposable Elements Drosophila Drosophila simulans Eye Gene Expression Regulation Genomics Green Fluorescent Proteins Mutagenesis, Insertional Species Specificity Transgenes Species of the Drosophila melanogaster species subgroup, including the species D. simulans, D. mauritiana, D. yakuba, and D. santomea, have long served as model systems for studying evolution. However, studies in these species have been limited by a paucity of genetic and transgenic reagents. Here, we describe a collection of transgenic and genetic strains generated to facilitate genetic studies within and between these species. We have generated many strains of each species containing mapped piggyBac transposons including an enhanced yellow fluorescent protein (EYFP) gene expressed in the eyes and a φC31 attP site-specific integration site. We have tested a subset of these lines for integration efficiency and reporter gene expression levels. We have also generated a smaller collection of other lines expressing other genetically encoded fluorescent molecules in the eyes and a number of other transgenic reagents that will be useful for functional studies in these species. In addition, we have mapped the insertion locations of 58 transposable elements in D. virilis that will be useful for genetic mapping studies. © 2017 Stern et al. Fil:Frankel, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_21601836_v7_n4_p1339_Stern |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Drosophila Evolution Genetics Speciation Transgenics φC31 integrase reagent yellow fluorescent protein enhanced green fluorescent protein green fluorescent protein Article controlled study Drosophila Drosophila mauritiana Drosophila santomea Drosophila simulans Drosophila virilis Drosophila yakuba gene expression genetic strain insect genetics nonhuman reporter gene transgene transposon allele animal Drosophila Drosophila simulans eye gene expression regulation genetics genomics metabolism species difference transgenic animal Alleles Animals Animals, Genetically Modified DNA Transposable Elements Drosophila Drosophila simulans Eye Gene Expression Regulation Genomics Green Fluorescent Proteins Mutagenesis, Insertional Species Specificity Transgenes |
| spellingShingle |
Drosophila Evolution Genetics Speciation Transgenics φC31 integrase reagent yellow fluorescent protein enhanced green fluorescent protein green fluorescent protein Article controlled study Drosophila Drosophila mauritiana Drosophila santomea Drosophila simulans Drosophila virilis Drosophila yakuba gene expression genetic strain insect genetics nonhuman reporter gene transgene transposon allele animal Drosophila Drosophila simulans eye gene expression regulation genetics genomics metabolism species difference transgenic animal Alleles Animals Animals, Genetically Modified DNA Transposable Elements Drosophila Drosophila simulans Eye Gene Expression Regulation Genomics Green Fluorescent Proteins Mutagenesis, Insertional Species Specificity Transgenes Stern, D.L. Crocker, J. Ding, Y. Frankel, N. Kappes, G. Kim, E. Kuzmickas, R. Lemire, A. Mast, J.D. Picard, S. Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis |
| topic_facet |
Drosophila Evolution Genetics Speciation Transgenics φC31 integrase reagent yellow fluorescent protein enhanced green fluorescent protein green fluorescent protein Article controlled study Drosophila Drosophila mauritiana Drosophila santomea Drosophila simulans Drosophila virilis Drosophila yakuba gene expression genetic strain insect genetics nonhuman reporter gene transgene transposon allele animal Drosophila Drosophila simulans eye gene expression regulation genetics genomics metabolism species difference transgenic animal Alleles Animals Animals, Genetically Modified DNA Transposable Elements Drosophila Drosophila simulans Eye Gene Expression Regulation Genomics Green Fluorescent Proteins Mutagenesis, Insertional Species Specificity Transgenes |
| description |
Species of the Drosophila melanogaster species subgroup, including the species D. simulans, D. mauritiana, D. yakuba, and D. santomea, have long served as model systems for studying evolution. However, studies in these species have been limited by a paucity of genetic and transgenic reagents. Here, we describe a collection of transgenic and genetic strains generated to facilitate genetic studies within and between these species. We have generated many strains of each species containing mapped piggyBac transposons including an enhanced yellow fluorescent protein (EYFP) gene expressed in the eyes and a φC31 attP site-specific integration site. We have tested a subset of these lines for integration efficiency and reporter gene expression levels. We have also generated a smaller collection of other lines expressing other genetically encoded fluorescent molecules in the eyes and a number of other transgenic reagents that will be useful for functional studies in these species. In addition, we have mapped the insertion locations of 58 transposable elements in D. virilis that will be useful for genetic mapping studies. © 2017 Stern et al. |
| format |
JOUR |
| author |
Stern, D.L. Crocker, J. Ding, Y. Frankel, N. Kappes, G. Kim, E. Kuzmickas, R. Lemire, A. Mast, J.D. Picard, S. |
| author_facet |
Stern, D.L. Crocker, J. Ding, Y. Frankel, N. Kappes, G. Kim, E. Kuzmickas, R. Lemire, A. Mast, J.D. Picard, S. |
| author_sort |
Stern, D.L. |
| title |
Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis |
| title_short |
Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis |
| title_full |
Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis |
| title_fullStr |
Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis |
| title_full_unstemmed |
Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis |
| title_sort |
genetic and transgenic reagents for drosophila simulans, d. mauritiana, d. yakuba, d. santomea, and d. virilis |
| url |
http://hdl.handle.net/20.500.12110/paper_21601836_v7_n4_p1339_Stern |
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