In situ pressure probe sampling of single cell solution from living plants for metabolite analyses with UV-MALDI MS

The joint application of a cell pressure probe (PP) and a UV-matrix-assisted laser desorption/ionization time of flight (UV-MALDI-TOF) mass spectrometer was used for picoliter sampling and shotgun metabolite profiling of in situ living single cells of plants. Plant cell wall and plasma membrane prop...

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Detalles Bibliográficos
Autores principales: Erra-Balsells, R., Gholipour, Y., Nonami, H., Gelman L., Hunter A., Korsunsky A.M., Ao S.I., Hukins D.WL.
Formato: CONF
Materias:
Metabolomics
Nanoparticles
Plant cell
Shotgun metabolite profiling
UV-MALDI-TOF
Biomolecules
Cell membranes
Desorption
Mass spectrometry
Metabolites
Plant cell culture
Plants (botany)
Potential flow
Probes
MALDI-TOF
MALDI-TOF mass spectrometry
Matrix-assisted laser desorption/ionization time of flights
Membrane properties
Metabolite analysis
Metabolite profiling
Plant cells
Cytology
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_20780958_v2197_n_p572_ErraBalsells
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:The joint application of a cell pressure probe (PP) and a UV-matrix-assisted laser desorption/ionization time of flight (UV-MALDI-TOF) mass spectrometer was used for picoliter sampling and shotgun metabolite profiling of in situ living single cells of plants. Plant cell wall and plasma membrane properties and water relations of in situ living single cells can be analyzed before sampling the cell sap. With different organic compounds and nanoparticles as UV-MALDI matrix, metabolites from neutral carbohydrates to amino acids and secondary other metabolites can be detected through UV-MALDI-TOF mass spectrometry analyses of picoliter single-cell samples. © 2012 Newswood Limited. All rights reserved.

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