In situ pressure probe sampling of single cell solution from living plants for metabolite analyses with UV-MALDI MS

The joint application of a cell pressure probe (PP) and a UV-matrix-assisted laser desorption/ionization time of flight (UV-MALDI-TOF) mass spectrometer was used for picoliter sampling and shotgun metabolite profiling of in situ living single cells of plants. Plant cell wall and plasma membrane prop...

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Autores principales: Erra-Balsells, R., Gholipour, Y., Nonami, H., Gelman L., Hunter A., Korsunsky A.M., Ao S.I., Hukins D.WL.
Formato: CONF
Materias:
Metabolomics
Nanoparticles
Plant cell
Shotgun metabolite profiling
UV-MALDI-TOF
Biomolecules
Cell membranes
Desorption
Mass spectrometry
Metabolites
Plant cell culture
Plants (botany)
Potential flow
Probes
MALDI-TOF
MALDI-TOF mass spectrometry
Matrix-assisted laser desorption/ionization time of flights
Membrane properties
Metabolite analysis
Metabolite profiling
Plant cells
Cytology
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_20780958_v2197_n_p572_ErraBalsells
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_20780958_v2197_n_p572_ErraBalsells
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spelling todo:paper_20780958_v2197_n_p572_ErraBalsells2023-10-03T16:39:18Z In situ pressure probe sampling of single cell solution from living plants for metabolite analyses with UV-MALDI MS Erra-Balsells, R. Gholipour, Y. Nonami, H. Gelman L. Hunter A. Korsunsky A.M. Ao S.I. Hukins D.WL. Metabolomics Nanoparticles Plant cell Shotgun metabolite profiling UV-MALDI-TOF Biomolecules Cell membranes Desorption Mass spectrometry Metabolites Nanoparticles Plant cell culture Plants (botany) Potential flow Probes MALDI-TOF MALDI-TOF mass spectrometry Matrix-assisted laser desorption/ionization time of flights Membrane properties Metabolite analysis Metabolite profiling Metabolomics Plant cells Cytology The joint application of a cell pressure probe (PP) and a UV-matrix-assisted laser desorption/ionization time of flight (UV-MALDI-TOF) mass spectrometer was used for picoliter sampling and shotgun metabolite profiling of in situ living single cells of plants. Plant cell wall and plasma membrane properties and water relations of in situ living single cells can be analyzed before sampling the cell sap. With different organic compounds and nanoparticles as UV-MALDI matrix, metabolites from neutral carbohydrates to amino acids and secondary other metabolites can be detected through UV-MALDI-TOF mass spectrometry analyses of picoliter single-cell samples. © 2012 Newswood Limited. All rights reserved. CONF info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_20780958_v2197_n_p572_ErraBalsells
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Metabolomics
Nanoparticles
Plant cell
Shotgun metabolite profiling
UV-MALDI-TOF
Biomolecules
Cell membranes
Desorption
Mass spectrometry
Metabolites
Nanoparticles
Plant cell culture
Plants (botany)
Potential flow
Probes
MALDI-TOF
MALDI-TOF mass spectrometry
Matrix-assisted laser desorption/ionization time of flights
Membrane properties
Metabolite analysis
Metabolite profiling
Metabolomics
Plant cells
Cytology
spellingShingle Metabolomics
Nanoparticles
Plant cell
Shotgun metabolite profiling
UV-MALDI-TOF
Biomolecules
Cell membranes
Desorption
Mass spectrometry
Metabolites
Nanoparticles
Plant cell culture
Plants (botany)
Potential flow
Probes
MALDI-TOF
MALDI-TOF mass spectrometry
Matrix-assisted laser desorption/ionization time of flights
Membrane properties
Metabolite analysis
Metabolite profiling
Metabolomics
Plant cells
Cytology
Erra-Balsells, R.
Gholipour, Y.
Nonami, H.
Gelman L.
Hunter A.
Korsunsky A.M.
Ao S.I.
Hukins D.WL.
In situ pressure probe sampling of single cell solution from living plants for metabolite analyses with UV-MALDI MS
topic_facet Metabolomics
Nanoparticles
Plant cell
Shotgun metabolite profiling
UV-MALDI-TOF
Biomolecules
Cell membranes
Desorption
Mass spectrometry
Metabolites
Nanoparticles
Plant cell culture
Plants (botany)
Potential flow
Probes
MALDI-TOF
MALDI-TOF mass spectrometry
Matrix-assisted laser desorption/ionization time of flights
Membrane properties
Metabolite analysis
Metabolite profiling
Metabolomics
Plant cells
Cytology
description The joint application of a cell pressure probe (PP) and a UV-matrix-assisted laser desorption/ionization time of flight (UV-MALDI-TOF) mass spectrometer was used for picoliter sampling and shotgun metabolite profiling of in situ living single cells of plants. Plant cell wall and plasma membrane properties and water relations of in situ living single cells can be analyzed before sampling the cell sap. With different organic compounds and nanoparticles as UV-MALDI matrix, metabolites from neutral carbohydrates to amino acids and secondary other metabolites can be detected through UV-MALDI-TOF mass spectrometry analyses of picoliter single-cell samples. © 2012 Newswood Limited. All rights reserved.
format CONF
author Erra-Balsells, R.
Gholipour, Y.
Nonami, H.
Gelman L.
Hunter A.
Korsunsky A.M.
Ao S.I.
Hukins D.WL.
author_facet Erra-Balsells, R.
Gholipour, Y.
Nonami, H.
Gelman L.
Hunter A.
Korsunsky A.M.
Ao S.I.
Hukins D.WL.
author_sort Erra-Balsells, R.
title In situ pressure probe sampling of single cell solution from living plants for metabolite analyses with UV-MALDI MS
title_short In situ pressure probe sampling of single cell solution from living plants for metabolite analyses with UV-MALDI MS
title_full In situ pressure probe sampling of single cell solution from living plants for metabolite analyses with UV-MALDI MS
title_fullStr In situ pressure probe sampling of single cell solution from living plants for metabolite analyses with UV-MALDI MS
title_full_unstemmed In situ pressure probe sampling of single cell solution from living plants for metabolite analyses with UV-MALDI MS
title_sort in situ pressure probe sampling of single cell solution from living plants for metabolite analyses with uv-maldi ms
url http://hdl.handle.net/20.500.12110/paper_20780958_v2197_n_p572_ErraBalsells
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