Lithium decreases the effects of neuronal calcium sensor protein 1 in pedunculopontine neurons
Human postmortem studies reported increased expression of neuronal calcium sensor protein 1 (NCS-1) in the brains of some bipolar disorder patients, and reduced or aberrant gamma band activity is present in the same disorder. Bipolar disorder is characterized by sleep dysregulation, suggesting a rol...
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todo:paper_2051817X_v4_n6_p1_DOnofrio2023-10-03T16:38:53Z Lithium decreases the effects of neuronal calcium sensor protein 1 in pedunculopontine neurons D'Onofrio, S. Urbano, F.J. Messias, E. Garcia-Rill, E. Bipolar disorder Gamma band activity Sleep/wake calcium channel N type frequenin calcium sensor proteins lithium derivative neuroleptic agent neuronal calcium sensor neuropeptide recombinant protein voltage-dependent calcium channel (P-Q type) animal calcium signaling channel gating dose response drug effects female gamma rhythm in vitro study kinetics male membrane potential metabolism nerve cell newborn pedunculopontine tegmental nucleus Sprague Dawley rat Animals Animals, Newborn Antipsychotic Agents Calcium Channels, N-Type Calcium Signaling Dose-Response Relationship, Drug Female Gamma Rhythm In Vitro Techniques Ion Channel Gating Kinetics Lithium Compounds Male Membrane Potentials Neuronal Calcium-Sensor Proteins Neurons Neuropeptides Pedunculopontine Tegmental Nucleus Rats, Sprague-Dawley Recombinant Proteins Human postmortem studies reported increased expression of neuronal calcium sensor protein 1 (NCS-1) in the brains of some bipolar disorder patients, and reduced or aberrant gamma band activity is present in the same disorder. Bipolar disorder is characterized by sleep dysregulation, suggesting a role for the reticular activating system (RAS). Lithium (Li+) has been shown to effectively treat the mood disturbances in bipolar disorder patients and was proposed to act by inhibiting the interaction between NCS-1 and inositol 1,4,5-triphosphate receptor protein (InsP3R). NCS-1 is known to enhance the activity of InsP3R, and of Ca2+-mediated gamma oscillatory activity in the pedunculopontine nucleus (PPN), part of the RAS. This study aimed to determine the nature of some of the intracellular mechanisms of Li+ on rat PPN cells and to identify the interaction between Li+ and NCS-1. Since Li+ has been shown to act by inhibiting the enhancing effects of NCS-1, we tested the hypothesis that Li+ would reduced the effects of overexpression of NCS-1 and prevent the downregulation of gamma band activity. Li+ decreased gamma oscillation frequency and amplitude by downregulating Ca2+ channel activity, whereas NCS-1 reduced the effect of Li+ on Ca2+ currents. These effects were mediated by a G-protein overinhibition of Ca2+ currents. These results suggest that Li+ affected intracellular pathways involving the activation of voltage-gated Ca2+ channels mediated by an intracellular mechanism involving voltage-dependent activation of G proteins, thereby normalizing gamma band oscillations mediated by P/Q-type calcium channels modulated by NCS-1. © 2016 Published by the American Physiological Society and The Physiological Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_2051817X_v4_n6_p1_DOnofrio |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bipolar disorder Gamma band activity Sleep/wake calcium channel N type frequenin calcium sensor proteins lithium derivative neuroleptic agent neuronal calcium sensor neuropeptide recombinant protein voltage-dependent calcium channel (P-Q type) animal calcium signaling channel gating dose response drug effects female gamma rhythm in vitro study kinetics male membrane potential metabolism nerve cell newborn pedunculopontine tegmental nucleus Sprague Dawley rat Animals Animals, Newborn Antipsychotic Agents Calcium Channels, N-Type Calcium Signaling Dose-Response Relationship, Drug Female Gamma Rhythm In Vitro Techniques Ion Channel Gating Kinetics Lithium Compounds Male Membrane Potentials Neuronal Calcium-Sensor Proteins Neurons Neuropeptides Pedunculopontine Tegmental Nucleus Rats, Sprague-Dawley Recombinant Proteins |
| spellingShingle |
Bipolar disorder Gamma band activity Sleep/wake calcium channel N type frequenin calcium sensor proteins lithium derivative neuroleptic agent neuronal calcium sensor neuropeptide recombinant protein voltage-dependent calcium channel (P-Q type) animal calcium signaling channel gating dose response drug effects female gamma rhythm in vitro study kinetics male membrane potential metabolism nerve cell newborn pedunculopontine tegmental nucleus Sprague Dawley rat Animals Animals, Newborn Antipsychotic Agents Calcium Channels, N-Type Calcium Signaling Dose-Response Relationship, Drug Female Gamma Rhythm In Vitro Techniques Ion Channel Gating Kinetics Lithium Compounds Male Membrane Potentials Neuronal Calcium-Sensor Proteins Neurons Neuropeptides Pedunculopontine Tegmental Nucleus Rats, Sprague-Dawley Recombinant Proteins D'Onofrio, S. Urbano, F.J. Messias, E. Garcia-Rill, E. Lithium decreases the effects of neuronal calcium sensor protein 1 in pedunculopontine neurons |
| topic_facet |
Bipolar disorder Gamma band activity Sleep/wake calcium channel N type frequenin calcium sensor proteins lithium derivative neuroleptic agent neuronal calcium sensor neuropeptide recombinant protein voltage-dependent calcium channel (P-Q type) animal calcium signaling channel gating dose response drug effects female gamma rhythm in vitro study kinetics male membrane potential metabolism nerve cell newborn pedunculopontine tegmental nucleus Sprague Dawley rat Animals Animals, Newborn Antipsychotic Agents Calcium Channels, N-Type Calcium Signaling Dose-Response Relationship, Drug Female Gamma Rhythm In Vitro Techniques Ion Channel Gating Kinetics Lithium Compounds Male Membrane Potentials Neuronal Calcium-Sensor Proteins Neurons Neuropeptides Pedunculopontine Tegmental Nucleus Rats, Sprague-Dawley Recombinant Proteins |
| description |
Human postmortem studies reported increased expression of neuronal calcium sensor protein 1 (NCS-1) in the brains of some bipolar disorder patients, and reduced or aberrant gamma band activity is present in the same disorder. Bipolar disorder is characterized by sleep dysregulation, suggesting a role for the reticular activating system (RAS). Lithium (Li+) has been shown to effectively treat the mood disturbances in bipolar disorder patients and was proposed to act by inhibiting the interaction between NCS-1 and inositol 1,4,5-triphosphate receptor protein (InsP3R). NCS-1 is known to enhance the activity of InsP3R, and of Ca2+-mediated gamma oscillatory activity in the pedunculopontine nucleus (PPN), part of the RAS. This study aimed to determine the nature of some of the intracellular mechanisms of Li+ on rat PPN cells and to identify the interaction between Li+ and NCS-1. Since Li+ has been shown to act by inhibiting the enhancing effects of NCS-1, we tested the hypothesis that Li+ would reduced the effects of overexpression of NCS-1 and prevent the downregulation of gamma band activity. Li+ decreased gamma oscillation frequency and amplitude by downregulating Ca2+ channel activity, whereas NCS-1 reduced the effect of Li+ on Ca2+ currents. These effects were mediated by a G-protein overinhibition of Ca2+ currents. These results suggest that Li+ affected intracellular pathways involving the activation of voltage-gated Ca2+ channels mediated by an intracellular mechanism involving voltage-dependent activation of G proteins, thereby normalizing gamma band oscillations mediated by P/Q-type calcium channels modulated by NCS-1. © 2016 Published by the American Physiological Society and The Physiological Society. |
| format |
JOUR |
| author |
D'Onofrio, S. Urbano, F.J. Messias, E. Garcia-Rill, E. |
| author_facet |
D'Onofrio, S. Urbano, F.J. Messias, E. Garcia-Rill, E. |
| author_sort |
D'Onofrio, S. |
| title |
Lithium decreases the effects of neuronal calcium sensor protein 1 in pedunculopontine neurons |
| title_short |
Lithium decreases the effects of neuronal calcium sensor protein 1 in pedunculopontine neurons |
| title_full |
Lithium decreases the effects of neuronal calcium sensor protein 1 in pedunculopontine neurons |
| title_fullStr |
Lithium decreases the effects of neuronal calcium sensor protein 1 in pedunculopontine neurons |
| title_full_unstemmed |
Lithium decreases the effects of neuronal calcium sensor protein 1 in pedunculopontine neurons |
| title_sort |
lithium decreases the effects of neuronal calcium sensor protein 1 in pedunculopontine neurons |
| url |
http://hdl.handle.net/20.500.12110/paper_2051817X_v4_n6_p1_DOnofrio |
| work_keys_str_mv |
AT donofrios lithiumdecreasestheeffectsofneuronalcalciumsensorprotein1inpedunculopontineneurons AT urbanofj lithiumdecreasestheeffectsofneuronalcalciumsensorprotein1inpedunculopontineneurons AT messiase lithiumdecreasestheeffectsofneuronalcalciumsensorprotein1inpedunculopontineneurons AT garciarille lithiumdecreasestheeffectsofneuronalcalciumsensorprotein1inpedunculopontineneurons |
| _version_ |
1807320348424667136 |