A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells

We report the synthesis of a near-infrared (NIR) fluorescent pH probe with a remarkable Stokes shift (∼135 nm) based on a tricarbocyanine (Cy-PIP). The fluorescent molecule was anchored to SiO2 nanoparticles (Cy-PIP@SiO2) and is capable of monitoring pH changes within the physiological range (pH 6-8...

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Detalles Bibliográficos
Autores principales: Toum Terrones, Y., Coluccio Leskow, F., Bordoni, A.V., Acebedo, S.L., Spagnuolo, C.C., Wolosiuk, A.
Formato: JOUR
Materias:
Infrared devices
Nanoparticles
Silica
Endocytic pathways
Fluorescence confocal microscopy
Fluorescent molecules
Fluorescent pH probes
Near-infrared fluorescence
pH fluctuations
Physiological range
SiO2 Nanoparticles
Fluorescence
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_20507518_v5_n22_p4031_ToumTerrones
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_20507518_v5_n22_p4031_ToumTerrones
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spelling todo:paper_20507518_v5_n22_p4031_ToumTerrones2023-10-03T16:38:49Z A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells Toum Terrones, Y. Coluccio Leskow, F. Bordoni, A.V. Acebedo, S.L. Spagnuolo, C.C. Wolosiuk, A. Infrared devices Nanoparticles Silica Endocytic pathways Fluorescence confocal microscopy Fluorescent molecules Fluorescent pH probes Near-infrared fluorescence pH fluctuations Physiological range SiO2 Nanoparticles Fluorescence We report the synthesis of a near-infrared (NIR) fluorescent pH probe with a remarkable Stokes shift (∼135 nm) based on a tricarbocyanine (Cy-PIP). The fluorescent molecule was anchored to SiO2 nanoparticles (Cy-PIP@SiO2) and is capable of monitoring pH changes within the physiological range (pH 6-8). The Cy-PIP@SiO2 nanoparticles were successfully internalized by HeLa cells as shown by fluorescence confocal microscopy, while flow cytometry revealed pH fluctuations during the endocytic pathway. © 2017 The Royal Society of Chemistry. Fil:Coluccio Leskow, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bordoni, A.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Acebedo, S.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Spagnuolo, C.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_20507518_v5_n22_p4031_ToumTerrones
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Infrared devices
Nanoparticles
Silica
Endocytic pathways
Fluorescence confocal microscopy
Fluorescent molecules
Fluorescent pH probes
Near-infrared fluorescence
pH fluctuations
Physiological range
SiO2 Nanoparticles
Fluorescence
spellingShingle Infrared devices
Nanoparticles
Silica
Endocytic pathways
Fluorescence confocal microscopy
Fluorescent molecules
Fluorescent pH probes
Near-infrared fluorescence
pH fluctuations
Physiological range
SiO2 Nanoparticles
Fluorescence
Toum Terrones, Y.
Coluccio Leskow, F.
Bordoni, A.V.
Acebedo, S.L.
Spagnuolo, C.C.
Wolosiuk, A.
A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells
topic_facet Infrared devices
Nanoparticles
Silica
Endocytic pathways
Fluorescence confocal microscopy
Fluorescent molecules
Fluorescent pH probes
Near-infrared fluorescence
pH fluctuations
Physiological range
SiO2 Nanoparticles
Fluorescence
description We report the synthesis of a near-infrared (NIR) fluorescent pH probe with a remarkable Stokes shift (∼135 nm) based on a tricarbocyanine (Cy-PIP). The fluorescent molecule was anchored to SiO2 nanoparticles (Cy-PIP@SiO2) and is capable of monitoring pH changes within the physiological range (pH 6-8). The Cy-PIP@SiO2 nanoparticles were successfully internalized by HeLa cells as shown by fluorescence confocal microscopy, while flow cytometry revealed pH fluctuations during the endocytic pathway. © 2017 The Royal Society of Chemistry.
format JOUR
author Toum Terrones, Y.
Coluccio Leskow, F.
Bordoni, A.V.
Acebedo, S.L.
Spagnuolo, C.C.
Wolosiuk, A.
author_facet Toum Terrones, Y.
Coluccio Leskow, F.
Bordoni, A.V.
Acebedo, S.L.
Spagnuolo, C.C.
Wolosiuk, A.
author_sort Toum Terrones, Y.
title A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells
title_short A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells
title_full A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells
title_fullStr A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells
title_full_unstemmed A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells
title_sort silica supported tricarbocyanine based ph nanosensor with a large stokes shift and a near infrared fluorescence response: performance in vitro and in live cells
url http://hdl.handle.net/20.500.12110/paper_20507518_v5_n22_p4031_ToumTerrones
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