Population genetics structure of glyphosate-resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance

Single sequence repeats (SSR) developed for Sorghum bicolor were used to characterize the genetic distance of 46 different Sorghum halepense (Johnsongrass) accessions from Argentina some of which have evolved toward glyphosate resistance. Since Johnsongrass is an allotetraploid and only one subgenom...

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Autores principales: Fernández, L., de Haro, L.A., Distefano, A.J., Martínez, M.C., Lía, V., Papa, J.C., Olea, I., Tosto, D., Hopp, H.E.
Formato: JOUR
Materias:
EPSPS
Glyphosate
Herbicide resistance
Microsatellites
Sorghum halepense
Weed epidemiology
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_20457758_v3_n10_p3388_Fernandez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_20457758_v3_n10_p3388_Fernandez2023-10-03T16:38:32Z Population genetics structure of glyphosate-resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance Fernández, L. de Haro, L.A. Distefano, A.J. Martínez, M.C. Lía, V. Papa, J.C. Olea, I. Tosto, D. Hopp, H.E. EPSPS Glyphosate Herbicide resistance Microsatellites Sorghum halepense Weed epidemiology Single sequence repeats (SSR) developed for Sorghum bicolor were used to characterize the genetic distance of 46 different Sorghum halepense (Johnsongrass) accessions from Argentina some of which have evolved toward glyphosate resistance. Since Johnsongrass is an allotetraploid and only one subgenome is homologous to cultivated sorghum, some SSR loci amplified up to two alleles while others (presumably more conserved loci) amplified up to four alleles. Twelve SSR providing information of 24 loci representative of Johnsongrass genome were selected for genetic distance characterization. All of them were highly polymorphic, which was evidenced by the number of different alleles found in the samples studied, in some of them up to 20. UPGMA and Mantel analysis showed that Johnsongrass glyphosate-resistant accessions that belong to different geographic regions do not share similar genetic backgrounds. In contrast, they show closer similarity to their neighboring susceptible counterparts. Discriminant Analysis of Principal Components using the clusters identified by K-means support the lack of a clear pattern of association among samples and resistance status or province of origin. Consequently, these results do not support a single genetic origin of glyphosate resistance. Nucleotide sequencing of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) encoding gene from glyphosate-resistant and susceptible accessions collected from different geographic origins showed that none presented expected mutations in aminoacid positions 101 and 106 which are diagnostic of target-site resistance mechanism. © 2013 The Authors. Fil:de Haro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Distefano, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Martínez, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lía, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tosto, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_20457758_v3_n10_p3388_Fernandez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic EPSPS
Glyphosate
Herbicide resistance
Microsatellites
Sorghum halepense
Weed epidemiology
spellingShingle EPSPS
Glyphosate
Herbicide resistance
Microsatellites
Sorghum halepense
Weed epidemiology
Fernández, L.
de Haro, L.A.
Distefano, A.J.
Martínez, M.C.
Lía, V.
Papa, J.C.
Olea, I.
Tosto, D.
Hopp, H.E.
Population genetics structure of glyphosate-resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance
topic_facet EPSPS
Glyphosate
Herbicide resistance
Microsatellites
Sorghum halepense
Weed epidemiology
description Single sequence repeats (SSR) developed for Sorghum bicolor were used to characterize the genetic distance of 46 different Sorghum halepense (Johnsongrass) accessions from Argentina some of which have evolved toward glyphosate resistance. Since Johnsongrass is an allotetraploid and only one subgenome is homologous to cultivated sorghum, some SSR loci amplified up to two alleles while others (presumably more conserved loci) amplified up to four alleles. Twelve SSR providing information of 24 loci representative of Johnsongrass genome were selected for genetic distance characterization. All of them were highly polymorphic, which was evidenced by the number of different alleles found in the samples studied, in some of them up to 20. UPGMA and Mantel analysis showed that Johnsongrass glyphosate-resistant accessions that belong to different geographic regions do not share similar genetic backgrounds. In contrast, they show closer similarity to their neighboring susceptible counterparts. Discriminant Analysis of Principal Components using the clusters identified by K-means support the lack of a clear pattern of association among samples and resistance status or province of origin. Consequently, these results do not support a single genetic origin of glyphosate resistance. Nucleotide sequencing of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) encoding gene from glyphosate-resistant and susceptible accessions collected from different geographic origins showed that none presented expected mutations in aminoacid positions 101 and 106 which are diagnostic of target-site resistance mechanism. © 2013 The Authors.
format JOUR
author Fernández, L.
de Haro, L.A.
Distefano, A.J.
Martínez, M.C.
Lía, V.
Papa, J.C.
Olea, I.
Tosto, D.
Hopp, H.E.
author_facet Fernández, L.
de Haro, L.A.
Distefano, A.J.
Martínez, M.C.
Lía, V.
Papa, J.C.
Olea, I.
Tosto, D.
Hopp, H.E.
author_sort Fernández, L.
title Population genetics structure of glyphosate-resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance
title_short Population genetics structure of glyphosate-resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance
title_full Population genetics structure of glyphosate-resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance
title_fullStr Population genetics structure of glyphosate-resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance
title_full_unstemmed Population genetics structure of glyphosate-resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance
title_sort population genetics structure of glyphosate-resistant johnsongrass (sorghum halepense l. pers) does not support a single origin of the resistance
url http://hdl.handle.net/20.500.12110/paper_20457758_v3_n10_p3388_Fernandez
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