Class II histone deacetylases require P/Q-type Ca 2+ channels and CaMKII to maintain gamma oscillations in the pedunculopontine nucleus

Epigenetic mechanisms (i.e., histone post-translational modification and DNA methylation) play a role in regulation of gene expression. The pedunculopontine nucleus (PPN), part of the reticular activating system, manifests intrinsic gamma oscillations generated by voltage-dependent, high threshold N...

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Autores principales: Urbano, F.J., Bisagno, V., Mahaffey, S., Lee, S.-H., Garcia-Rill, E.
Formato: JOUR
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_20452322_v8_n1_p_Urbano
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_20452322_v8_n1_p_Urbano2023-10-03T16:38:29Z Class II histone deacetylases require P/Q-type Ca 2+ channels and CaMKII to maintain gamma oscillations in the pedunculopontine nucleus Urbano, F.J. Bisagno, V. Mahaffey, S. Lee, S.-H. Garcia-Rill, E. Epigenetic mechanisms (i.e., histone post-translational modification and DNA methylation) play a role in regulation of gene expression. The pedunculopontine nucleus (PPN), part of the reticular activating system, manifests intrinsic gamma oscillations generated by voltage-dependent, high threshold N- and P/Q-type Ca 2+ channels. We studied whether PPN intrinsic gamma oscillations are affected by inhibition of histone deacetylation. We showed that, a) acute in vitro exposure to the histone deacetylation Class I and II inhibitor trichostatin A (TSA, 1 μM) eliminated oscillations in the gamma range, but not lower frequencies, b) pre-incubation with TSA (1 μM, 90–120 min) also decreased gamma oscillations, c) Ca 2+ currents (I Ca ) were reduced by TSA, especially on cells with P/Q-type channels, d) a HDAC Class I inhibitor MS275 (500 nM), and a Class IIb inhibitor Tubastatin A (150–500 nM), failed to affect gamma oscillations, e) MC1568, a HDAC Class IIa inhibitor (1 μM), blocked gamma oscillations, and f) the effects of both TSA and MC1568 were blunted by blockade of CaMKII with KN-93 (1 μM). These results suggest a cell type specific effect on gamma oscillations when histone deacetylation is blocked, suggesting that gamma oscillations through P/Q-type channels modulated by CaMKII may be linked to processes related to gene transcription. © 2018, The Author(s). JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_20452322_v8_n1_p_Urbano
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description Epigenetic mechanisms (i.e., histone post-translational modification and DNA methylation) play a role in regulation of gene expression. The pedunculopontine nucleus (PPN), part of the reticular activating system, manifests intrinsic gamma oscillations generated by voltage-dependent, high threshold N- and P/Q-type Ca 2+ channels. We studied whether PPN intrinsic gamma oscillations are affected by inhibition of histone deacetylation. We showed that, a) acute in vitro exposure to the histone deacetylation Class I and II inhibitor trichostatin A (TSA, 1 μM) eliminated oscillations in the gamma range, but not lower frequencies, b) pre-incubation with TSA (1 μM, 90–120 min) also decreased gamma oscillations, c) Ca 2+ currents (I Ca ) were reduced by TSA, especially on cells with P/Q-type channels, d) a HDAC Class I inhibitor MS275 (500 nM), and a Class IIb inhibitor Tubastatin A (150–500 nM), failed to affect gamma oscillations, e) MC1568, a HDAC Class IIa inhibitor (1 μM), blocked gamma oscillations, and f) the effects of both TSA and MC1568 were blunted by blockade of CaMKII with KN-93 (1 μM). These results suggest a cell type specific effect on gamma oscillations when histone deacetylation is blocked, suggesting that gamma oscillations through P/Q-type channels modulated by CaMKII may be linked to processes related to gene transcription. © 2018, The Author(s).
format JOUR
author Urbano, F.J.
Bisagno, V.
Mahaffey, S.
Lee, S.-H.
Garcia-Rill, E.
spellingShingle Urbano, F.J.
Bisagno, V.
Mahaffey, S.
Lee, S.-H.
Garcia-Rill, E.
Class II histone deacetylases require P/Q-type Ca 2+ channels and CaMKII to maintain gamma oscillations in the pedunculopontine nucleus
author_facet Urbano, F.J.
Bisagno, V.
Mahaffey, S.
Lee, S.-H.
Garcia-Rill, E.
author_sort Urbano, F.J.
title Class II histone deacetylases require P/Q-type Ca 2+ channels and CaMKII to maintain gamma oscillations in the pedunculopontine nucleus
title_short Class II histone deacetylases require P/Q-type Ca 2+ channels and CaMKII to maintain gamma oscillations in the pedunculopontine nucleus
title_full Class II histone deacetylases require P/Q-type Ca 2+ channels and CaMKII to maintain gamma oscillations in the pedunculopontine nucleus
title_fullStr Class II histone deacetylases require P/Q-type Ca 2+ channels and CaMKII to maintain gamma oscillations in the pedunculopontine nucleus
title_full_unstemmed Class II histone deacetylases require P/Q-type Ca 2+ channels and CaMKII to maintain gamma oscillations in the pedunculopontine nucleus
title_sort class ii histone deacetylases require p/q-type ca 2+ channels and camkii to maintain gamma oscillations in the pedunculopontine nucleus
url http://hdl.handle.net/20.500.12110/paper_20452322_v8_n1_p_Urbano
work_keys_str_mv AT urbanofj classiihistonedeacetylasesrequirepqtypeca2channelsandcamkiitomaintaingammaoscillationsinthepedunculopontinenucleus
AT bisagnov classiihistonedeacetylasesrequirepqtypeca2channelsandcamkiitomaintaingammaoscillationsinthepedunculopontinenucleus
AT mahaffeys classiihistonedeacetylasesrequirepqtypeca2channelsandcamkiitomaintaingammaoscillationsinthepedunculopontinenucleus
AT leesh classiihistonedeacetylasesrequirepqtypeca2channelsandcamkiitomaintaingammaoscillationsinthepedunculopontinenucleus
AT garciarille classiihistonedeacetylasesrequirepqtypeca2channelsandcamkiitomaintaingammaoscillationsinthepedunculopontinenucleus
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