Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD
Extensins are plant cell wall glycoproteins that act as scaffolds for the deposition of the main wall carbohydrate polymers, which are interlocked into the supramolecular wall structure through intra- and inter-molecular iso-di-tyrosine crosslinks within the extensin backbone. In the conserved canon...
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todo:paper_20452322_v7_n_p_Moller2023-10-03T16:38:21Z Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD Møller, S.R. Yi, X. Velásquez, S.M. Gille, S. Hansen, P.L.M. Poulsen, C.P. Olsen, C.E. Rejzek, M. Parsons, H. Zhang, Y. Wandall, H.H. Clausen, H. Field, R.A. Pauly, M. Estevez, J.M. Harholt, J. Ulvskov, P. Petersen, B.L. Arabidopsis protein arabinose ARAf protein, Arabidopsis bacterial DNA glycoprotein glycosyltransferase glycosyltransferase T-DNA xylan 1,4 beta xylosidase anatomy and histology Arabidopsis cell wall enzymology gene knockout genetics glycosylation growth, development and aging metabolism molecular evolution mutation plant root Arabidopsis Arabidopsis Proteins Arabinose Cell Wall DNA, Bacterial Evolution, Molecular Gene Knockout Techniques Glycosylation Hexosyltransferases Mutation Plant Roots Xylosidases Extensins are plant cell wall glycoproteins that act as scaffolds for the deposition of the main wall carbohydrate polymers, which are interlocked into the supramolecular wall structure through intra- and inter-molecular iso-di-tyrosine crosslinks within the extensin backbone. In the conserved canonical extensin repeat, Ser-Hyp 4, serine and the consecutive C4-hydroxyprolines (Hyps) are substituted with an α-galactose and 1-5 β- or α-linked arabinofuranoses (Arafs), respectively. These modifications are required for correct extended structure and function of the extensin network. Here, we identified a single Arabidopsis thaliana gene, At3g57630, in clade E of the inverting Glycosyltransferase family GT47 as a candidate for the transfer of Araf to Hyp-arabinofuranotriose (Hyp-β1,4Araf-β1,2Araf-β1,2Araf) side chains in an α-linkage, to yield Hyp-Araf 4 which is exclusively found in extensins. T-DNA knock-out mutants of At3g57630 showed a truncated root hair phenotype, as seen for mutants of all hitherto characterized extensin glycosylation enzymes; both root hair and glycan phenotypes were restored upon reintroduction of At3g57630. At3g57630 was named Extensin Arabinose Deficient transferase, ExAD, accordingly. The occurrence of ExAD orthologs within the Viridiplantae along with its' product, Hyp-Araf 4, point to ExAD being an evolutionary hallmark of terrestrial plants and charophyte green algae. © The Author(s) 2017. Fil:Velásquez, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_20452322_v7_n_p_Moller |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arabidopsis protein arabinose ARAf protein, Arabidopsis bacterial DNA glycoprotein glycosyltransferase glycosyltransferase T-DNA xylan 1,4 beta xylosidase anatomy and histology Arabidopsis cell wall enzymology gene knockout genetics glycosylation growth, development and aging metabolism molecular evolution mutation plant root Arabidopsis Arabidopsis Proteins Arabinose Cell Wall DNA, Bacterial Evolution, Molecular Gene Knockout Techniques Glycosylation Hexosyltransferases Mutation Plant Roots Xylosidases |
| spellingShingle |
Arabidopsis protein arabinose ARAf protein, Arabidopsis bacterial DNA glycoprotein glycosyltransferase glycosyltransferase T-DNA xylan 1,4 beta xylosidase anatomy and histology Arabidopsis cell wall enzymology gene knockout genetics glycosylation growth, development and aging metabolism molecular evolution mutation plant root Arabidopsis Arabidopsis Proteins Arabinose Cell Wall DNA, Bacterial Evolution, Molecular Gene Knockout Techniques Glycosylation Hexosyltransferases Mutation Plant Roots Xylosidases Møller, S.R. Yi, X. Velásquez, S.M. Gille, S. Hansen, P.L.M. Poulsen, C.P. Olsen, C.E. Rejzek, M. Parsons, H. Zhang, Y. Wandall, H.H. Clausen, H. Field, R.A. Pauly, M. Estevez, J.M. Harholt, J. Ulvskov, P. Petersen, B.L. Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD |
| topic_facet |
Arabidopsis protein arabinose ARAf protein, Arabidopsis bacterial DNA glycoprotein glycosyltransferase glycosyltransferase T-DNA xylan 1,4 beta xylosidase anatomy and histology Arabidopsis cell wall enzymology gene knockout genetics glycosylation growth, development and aging metabolism molecular evolution mutation plant root Arabidopsis Arabidopsis Proteins Arabinose Cell Wall DNA, Bacterial Evolution, Molecular Gene Knockout Techniques Glycosylation Hexosyltransferases Mutation Plant Roots Xylosidases |
| description |
Extensins are plant cell wall glycoproteins that act as scaffolds for the deposition of the main wall carbohydrate polymers, which are interlocked into the supramolecular wall structure through intra- and inter-molecular iso-di-tyrosine crosslinks within the extensin backbone. In the conserved canonical extensin repeat, Ser-Hyp 4, serine and the consecutive C4-hydroxyprolines (Hyps) are substituted with an α-galactose and 1-5 β- or α-linked arabinofuranoses (Arafs), respectively. These modifications are required for correct extended structure and function of the extensin network. Here, we identified a single Arabidopsis thaliana gene, At3g57630, in clade E of the inverting Glycosyltransferase family GT47 as a candidate for the transfer of Araf to Hyp-arabinofuranotriose (Hyp-β1,4Araf-β1,2Araf-β1,2Araf) side chains in an α-linkage, to yield Hyp-Araf 4 which is exclusively found in extensins. T-DNA knock-out mutants of At3g57630 showed a truncated root hair phenotype, as seen for mutants of all hitherto characterized extensin glycosylation enzymes; both root hair and glycan phenotypes were restored upon reintroduction of At3g57630. At3g57630 was named Extensin Arabinose Deficient transferase, ExAD, accordingly. The occurrence of ExAD orthologs within the Viridiplantae along with its' product, Hyp-Araf 4, point to ExAD being an evolutionary hallmark of terrestrial plants and charophyte green algae. © The Author(s) 2017. |
| format |
JOUR |
| author |
Møller, S.R. Yi, X. Velásquez, S.M. Gille, S. Hansen, P.L.M. Poulsen, C.P. Olsen, C.E. Rejzek, M. Parsons, H. Zhang, Y. Wandall, H.H. Clausen, H. Field, R.A. Pauly, M. Estevez, J.M. Harholt, J. Ulvskov, P. Petersen, B.L. |
| author_facet |
Møller, S.R. Yi, X. Velásquez, S.M. Gille, S. Hansen, P.L.M. Poulsen, C.P. Olsen, C.E. Rejzek, M. Parsons, H. Zhang, Y. Wandall, H.H. Clausen, H. Field, R.A. Pauly, M. Estevez, J.M. Harholt, J. Ulvskov, P. Petersen, B.L. |
| author_sort |
Møller, S.R. |
| title |
Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD |
| title_short |
Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD |
| title_full |
Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD |
| title_fullStr |
Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD |
| title_full_unstemmed |
Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD |
| title_sort |
identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, exad |
| url |
http://hdl.handle.net/20.500.12110/paper_20452322_v7_n_p_Moller |
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1807321819287388160 |